Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2007 (vol. 55), issue 2

Identifikace a charakterizace bakterií rodu Enterococcus určených pro výrobu funkčních potravin

Identification and typization of bacteria of the genus Enterococcus supposed to be used for the production of functional foods

Radka Burdychová

Acta Univ. Agric. Silvic. Mendelianae Brun. 2007, 55(2), 9-14 | DOI: 10.11118/actaun200755020009  

In this study, the species identification of 12 probiotic strains of the genus Enterococcus from Culture Collection of Dairy Microorganisms Lactoflora (CCDM, Milcom, Tábor, Czech Republic) were done using PCR described by DUTKA-MALEN et al. (1995). All strains were classified to be of the genus Enterococcus and species E. faecium. These strains are supposed to be used as probiotics for the production of functional foods. According to the fact that E. faecium was described to have decarboxylase activity responsible for biogenic amine production in fermented products, the presence of genes coding for microbial tyrosine and...

Mikrobiologická detekce probiotických mikroorganismů ve fermentovaných mléčných výrobcích

Microbiological detection of probiotic microorganisms in fermented milk products

Radka Burdychová

Acta Univ. Agric. Silvic. Mendelianae Brun. 2007, 55(2), 15-20 | DOI: 10.11118/actaun200755020015  

A number of health benefits have been claimed for probiotic bacteria such as Lactobacillus acidophilus, Bifidobacterium spp. and Lactobacillus rhamnosus. Because of the potential health benefits, these organisms are increasingly incorporated into dairy foods. However, to reach health benefits, the concentration of probiotics have to be 106 CFU/g of a product. For assessing of required probiotic bacteria quantity, it is important to have a working method for selective enumeration of these probiotic bacteria.Five bacteriological media were evaluated to assess their suitability to selectively enumerate Streptococcus...

Hodnocení strojů pro defoliaci vinic s ohledem na kvalitu práce

The classification of defoliators in relation to damage and quality of bunches

Patrik Burg

Acta Univ. Agric. Silvic. Mendelianae Brun. 2007, 55(2), 21-26 | DOI: 10.11118/actaun200755020021  

Defoliation of vineyard find between growers always wider exercise. The ground are ecological aspects and for ever growing title to qualitative character of production. Thrifty ablation of leaves from winegrapes zone, fulfilment in correct term highly effects on health of clusters and quantity of their contentual matters.The aim of this work was monitoring of technic-economical parameters by 2 sets with defoliators. Furthemore was evaluated quality of their work in relation to quantity of involved bunches.

Vliv vybraných faktorů na růstovou schopnost jehňat kříženců s využitím plemene Suffolk v otcovské pozici

Effect of chosen factors on growth of lambs crossbreeds with using Suffolk in sire position

Igor Dobeš, Jan Kuchtík, Radim Petr, Radek Filipčík

Acta Univ. Agric. Silvic. Mendelianae Brun. 2007, 55(2), 27-32 | DOI: 10.11118/actaun200755020027  

The aim of the study was evaluated the effect of chosen factors (sex, litter size, year of the study) on the growth of lambs crossbreeds with using Suffolk (Sf) in the sire position. In the mother position there were used following genotypes: Charollais (Ch), Sf 50 Ch, Improved Wallachian (IW) and IW 50 Sf. The evaluation was carried out during two successive years (2004-2005) on the farm Růžďka and involved altogether 141 lambs. The growth of lambs was evaluated in the following crossbreeds: Ch 50 Sf (n = 38), Sf 75 Ch (n = 40), IW 50 Sf (n = 29) and Sf 75 IW (n = 34). All lambs were born indoor in the period from January until the end of first decade...

Monitoring výskytu drobných hlodavců a související zdravotní rizika v odchovně lovné pernaté zvěře

Surveillance of small rodents and related health risks in a game bird farm

Jana Horáková, Hana Banďouchová, Jiří Pikula

Acta Univ. Agric. Silvic. Mendelianae Brun. 2007, 55(2), 33-42 | DOI: 10.11118/actaun200755020033  

Using live trapping devices we performed surveillance of small vertebrates in the game bird farm in Jinačovice in 2005 to 2006. The study was aimed at determining the species composition and numbers within individual technologies of rearing as well as finding possible reservoirs or sources of infectious agents. Five study areas were examined in 2005 and other three located within buildings were added in 2006. A line of 10 traps approximately 2 metres apart was employed on each study area. Small mammals trapped were sampled for parasitology and serology.Until the present, a total of 92 common species were captured - i.e., yellow-necked fieldmouse...

Tvorba syntetické mapy zranitelnosti podzemní vody na příkladu povodí potoka Žilůvky

The construction of synthetic maps of groundwater vulnerability on the example of catchment area of Žilůvky stream

Kristýna Kubová, Tomáš Mikita

Acta Univ. Agric. Silvic. Mendelianae Brun. 2007, 55(2), 43-52 | DOI: 10.11118/actaun200755020043  

The term "vulnerability of groundwater to contamination" was introduced by French hydrogeologist J. Margat in the late 1960s. The idea of describing the degree of vulnerability of groundwater to contaminants as a function of hydrogeological conditions by means of maps was conceived to show that the protection provided by the natural environment varies at different locations. Groundwater vulnerability maps belong to category of special-purpose environmental maps and introduce one of the possible tool to solve groundwater protection. The synthetic map of relevant catchment area of Žilůvky stream was composed in the program ArcGIS as a intersection between...

Hodnotenie dynamiky rastu klonov Lonicera kamtschatica

The evaluation of growth dynamics of Lonicera kamtschatica clones

Ján Matuškovič, Tünde Pokorná-Juríková

Acta Univ. Agric. Silvic. Mendelianae Brun. 2007, 55(2), 53-58 | DOI: 10.11118/actaun200755020053  

Artickle deals with the evaluation of growth dynamics of selected set of clones Lonicera kamtschatica in the conditions of Nitra. We measured the growth of the shrubs twice a year (in spring and autumn) during 2003-2005. Within all clones 5 shrubs were evaluated. On the basis of the obtained results we can claim the highest increase of height in case of LKL 21 followed by clones LKL 16 and LKL 5. The lowest growth increase was typical for LKL 58 and LKL 66.In term of statistical evaluation the year can be considered as a statistically significant factor forming a growth intensity of clones during 2003-2005. The effect of year on growing...

Vliv počtu somatických buněk na SLOŽENÍ A VLASTNOSTI ovčího mléka A NA JAKOST SÝŘENINY

Effect of somatic cell count on ewe's milk composition, its properties and the quality of rennet curd

Lenka Novotná, Jan Kuchtík, Igor Dobeš, Květoslava Šustová, Pavlína Zajícová

Acta Univ. Agric. Silvic. Mendelianae Brun. 2007, 55(2), 59-64 | DOI: 10.11118/actaun200755020059  

The evaluation of the effect of somatic cells count on ewe's milk composition, its properties and on the quality of rennet curd was carried using milk samples obtained from a total 10 ewes F112 crossbreeds of Lacaune (L), East Friesian (EF) and Improved Wallachian (IW), reared on a ecological sheep farm in Valašská Bystřice. All ewes were on the 1st lactation, in good nutritive condition and clinically healthy. The sampling was carried on 47th, 81st, 123rd, 152nd and 189th day of lactation. The analysis involved a total of 50 milk samples, which were categorized into 5 different...

Monitorování změn integrity chromatinu v populaci motilních bovinních spermií kapacitovaných v podmínkách in vitro

Monitoring of chromatin integrity changes in the population of motile bovine sperm capacitated in vitro

Zuzana Rečková, Marie Machatková, Roman Rybář, Ladislav Máchal

Acta Univ. Agric. Silvic. Mendelianae Brun. 2007, 55(2), 65-70 | DOI: 10.11118/actaun200755020065  

The objective of our study was to standardize a method for chromatin integrity assessment in a separated population of bovine sperm and monitor the changes occurring during sperm capacitation stimulated with heparin. Frozen sperm of 11 young bulls of the Czech pied breed with a defined fertility in both in vitro system (from 12.9% to 25.8% embryos) and in insemination (from 60.2% to 66.4% pregnancy) was used in our experiments.Bovine spermatozoa were isolated by Percoll gradient centrifugation from frozen-thawed semen using Tyrode's medium (SP-Talp) and resuspended in a fertilization medium (IVF-Talp). The spermatozoa were incubated at laboratory...

Vliv porušení chladírenského řetězce na mikrobiologickou kvalitu pasterovaného mléka

Breaking of cooling chain influencing microbial quality of pasteurized milk

Pavla Sládková, Květoslava Šustová, Radka Burdychová

Acta Univ. Agric. Silvic. Mendelianae Brun. 2007, 55(2), 71-76 | DOI: 10.11118/actaun200755020071  

The aim of this thesis was to evaluate the changes of the microbiological quality of milk during storage. Fresh pasteurized milk was used for the laboratory experimentations. Cooling chain of the samples was broken for 8 and for 12 hours at different temperatures (22-30 °C). In these samples, total count of psychrotrophic microorganisms was assessed.Technological indiscipline, i. e. higher storage temperature (24 °C and more), was associated with higher increase of total count of microorganisms and psychrotrophic microorganisms. Microbiological results proved that the samples stored under standard conditions were of very good quality. However,...

Vodní bezobratlí mikulovských rybníků

Aquatic invertebrate of mikulovské rybníky ponds

Ivo Sukop

Acta Univ. Agric. Silvic. Mendelianae Brun. 2007, 55(2), 77-84 | DOI: 10.11118/actaun200755020077  

The qualitative composition of aquatic invertebrates and zooplankton abundance of the Mikulovské rybníky ponds is presented in the study. Size and species composition of zooplankton in studied ponds was influenced by high fish stocks in 1992-1994. In many cases, small taxa (Rotatoria, copepod Acanthocyclops robustus and cladoceran Bosmina longirostris) formed the zooplankton assemblage. Large zooplankton species (e.g. Daphnia magna) dominated only occasionaly and exclusively in ponds with low fish stock.

Zoobentos Lednických rybníků

Zoobenthos of the Lednické rybníky ponds

Ivo Sukop

Acta Univ. Agric. Silvic. Mendelianae Brun. 2007, 55(2), 85-94 | DOI: 10.11118/actaun200755020085  

Qualitative and quantitative composition of macrozoobenthos was studied on the lednické rybníky ponds. Altogether 299 taxa of benthic macroinvertebrates were determined. The values of macrozoobenthos density and biomass ranged between 5-13 021 ind.m-2 and 0.02-79.6 g.m-2 respectively.

Výskyt Asijské rzi Puccinia bornmuelleri Magnus v České republice

Occurence of Puccinia bornmuelleri Magnus in the Czech Republic

Jiří Müller, Ivana Šafránková

Acta Univ. Agric. Silvic. Mendelianae Brun. 2007, 55(2), 95-98 | DOI: 10.11118/actaun200755020095  

Rust on Levisticum officinale was found on the foothill of "Velká Baba" Hill north-west of Brno-Řečkovice on May 2006. It was identifity as Puccinia bornmuelleri Magnus. On the leaves there were a lot of uredinia and one group of spermogonia. The telia was found in September 2006. Probably, the rust occurs on more locations in Brno. The rust was found in Rumania a few years ago. These are the first two findings in Europe. The rust comes from Iran and Afghanistan. Spermogonia have been found together with uredia therefore P. bornmuelleri is brachypuccinia.

Potenciál tvrdosti zrna ječmene (Hordeum vulgare L.) jako selekčního znaku v procesu šlechtění

Potential of grain hardness of barley (Hordeum vulgare L.) as a selection trait in breeding programme

Karel Vejražka, Vratislav Psota, Jaroslava Ehrenbergerová, Natálie Březinová Belcredi, Radim Cerkal

Acta Univ. Agric. Silvic. Mendelianae Brun. 2007, 55(2), 99-104 | DOI: 10.11118/actaun200755020099  

The study presents the results of research on the use of barley caryopses hardness for prediction of the malting quality. Grain hardness was determined by three methods: Particle size index (PSI), hardness according to Brabender (BRA) and grain milling energy (GME). The correlations among methods for determining the kernel hardness were estimated as well among selected technological traits of barley and malt and kernel hardness. Significant correlations were found among PSI values and Final attenuation of laboratory wort from malt (0.73*) a Glycidic extract (0.70*). Values of kernel hardness measured by BRA significantly correlated with Final attenuation...

VALIDACE METOD PRO STANOVENÍ OBSAHU VOLNÉ VODY V DRŮBEŽÍM MASE

Validation of methods for determination of free water content in poultry meat

Jarmila Žítková, Jana Simeonovová

Acta Univ. Agric. Silvic. Mendelianae Brun. 2007, 55(2), 105-118 | DOI: 10.11118/actaun200755020105  

Methods for determination of free water content in poultry meat are described in Commission Regulation EEC No 1538/91 as amended and in ČSN 57 3100. Two of them (method A and D) have been validated in conditions of a Czech poultry processing plant. The capacity of slaughtering was 6000 pieces per hour and carcasses were chilled by air with spraying. All determinations were carried out in the plant's lab and in the lab of the Institute of Food Technology. Method A was used to detect the amount of water lost from frozen chicken during thawing in controlled conditions. Twenty carcasses from six weight groups (900 g-1400 g) were tested. The average values...

Překročení kritických úrovní ozonu na stanici monitoringu čistoty ovzduší v areálu BZA MZLU v Brně v letech 2001 až 2006

Tronsgression of critical ozone level at monitoring station for air eleanness in BZA MZLU for period 2001-2006

Jan Svoboda

Acta Univ. Agric. Silvic. Mendelianae Brun. 2007, 55(2), 123-130 | DOI: 10.11118/actaun200755020123  

Cumulative effects of agricultural crops exposition, forests and other ecosystems for ozone concentration above the threshold is characterized by exposition index AOT. According to experst for EHK, the recent level is 40 ppb. Very dry summer in 2006 and 2003 created good conditions for photochemical reaction as well as ozone transport in Central Europe. Such years can be taken as an example in connection with expected concentration of ground ozone (as a result of global warming). Amount of ozone in troposphere is measured in Botanic garden and MZLU arboretum areas since 2001 by analyzator 41M-LCD. Ozone course correlates with course of air temperature...