Acta Univ. Agric. Silvic. Mendelianae Brun. 2014, 62(5), 919-927 | DOI: 10.11118/actaun201462050919

Development in Indirect Infra-red Determination of Milk Acetone

Oto Hanuš1, Petr Roubal1, Jan Říha1, Marcela Vyletělová Klimešová1, Eva Samková2, Radoslava Jedelská1, Jaroslav Kopecký1
1 Dairy Research Institute, Ltd., Prague, Ke Dvoru 12a, 160 00 Prague 6-Vokovice, Czech Republic
2 Faculty of Agriculture, University of South Bohemia České Budějovice, Branišovská 1, 370 05 České Budějovice, Czech Republic

Milk acetone (AC) is an indicator of energy metabolism of cows and ketosis occurrence. AC result inerpretation is essential for prevention and treatment in dairy cow herds. There is necessary an effective method with reliable results. The goal was to evaluate the mid infrared method MIR-FT in terms of calibration for AC. Microdiffusion photometric (485 nm) method with salicylaldehyde as reference (RE) and MIR-FT (Lactoscope FT-IR, Delta (D); MilkoScan FT 6000 (F); Bentley (Bentley Instruments (B)) as indirect method were used. Selected (from high yielding dairy cows in early lactation) individual milk samples (MSs; n = 89) were used for MIR-FT calibration development and evaluation. Log AC correlation (r) between RE and indirect MIR-FT (D) was low (0.22, P < 0.05). The same parameter between RE and MIR-FT (F) was closer (0.589, P < 0.001; 0.632, P < 0.001 for n = 64. The artificial AC addition to milk samples had no visible effect on AC recovery by MIR-FT instruments. The AC values increased from 4.91 and 5.23 to 45.22 mg.l-1 by RE. There is no possibility to prepare the AC reference samples using artificial addition for MIR-FT calibration. In dependence on possible AC evaporation (a risk of AC result reduction) during storage conditions a knowlege about AC stability in sample is important. The similar AC results were obtained after milk sampling and after 48 hours of storage under cold conditions. This is new information for analytical work.

Keywords: raw cow milk, acetone, ketosis, photometry, infrared spectroscopy, calibration
Grants and funding:

This paper was supported by projects RO1414, NAZV QH81105 and MSM 6007665806. Authors thank to Mr. Pavel Kopunecz, David Ledvina, Zdeněk Motyčka and Jan Zlatníček from Czech-Moravia Breeders Corporation for their kind support and technical cooperation.

Published: December 2, 2014  Show citation

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Hanuš, O., Roubal, P., Říha, J., Vyletělová Klimešová, M., Samková, E., Jedelská, R., & Kopecký, J. (2014). Development in Indirect Infra-red Determination of Milk Acetone. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 62(5), 919-927. doi: 10.11118/actaun201462050919
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