Acta Univ. Agric. Silvic. Mendelianae Brun. 2013, 61(6), 1693-1701 | DOI: 10.11118/actaun201361061693

Assessment of antioxidant activity and total polyphenolic compounds of peach varieties infected with the Plum pox virus

Jana Horsáková1, Jiří Sochor2, Boris Krška1
1 Department of Fruit Growing, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech Republic
2 Department of Chemistry and Biochemistry, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic

Just like in other stone fruits, also in peach trees, the Plum pox virus is commonly known to be the cause of lower yields, worse quality and smaller size of fruits and it also affects the contained substances. The fruits of peach trees infected with the Plum pox virus (PPV) were subjected to various analyses to determine the content of antioxidant activity and overall polyphenolic compounds. The evaluation took place from 2011 to 2012. To conduct this experiment, two cultivars that had been infected by PPV naturally were selected - 'Royal Glory' and 'Symphony'. Antioxidant activity was established using five principally different methods (DPPH, ABTS, FRAP, DMPD and Free Radicals). The content of total polyphenolic compounds was established using the Folin-Ciocalteu method. The results of these analyses are expressed as the equivalent of gallic acid (GAE) in mg.kg-1. Furthermore, in the period from April to October the intensity of PPV symptoms in blossoms, leaves and fruits was also assessed.
It was discovered that as a result of the PPV infection, the content of antioxidant activity as well as of total polyphenols had increased. Average reading of antioxidant activity in the PPV infected fruits had increased by 13.2 % (DPPH), 26.7 % (FRAP), 27.6 % (ABTS), 28.1 % (DMPD), 39.2 % (Free Radicals) and the content of polyphenolic compounds had gone up by about 30.4 % in comparison with the control varieties.

Keywords: peaches, antioxidant activity, polyphenolic compounds, Plum pox virus

Received: June 12, 2013; Published: November 24, 2013  Show citation

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Horsáková, J., Sochor, J., & Krška, B. (2013). Assessment of antioxidant activity and total polyphenolic compounds of peach varieties infected with the Plum pox virus. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 61(6), 1693-1701. doi: 10.11118/actaun201361061693
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    References

    1. ASADA, K., 1999: The water-water cycle in chloroplasts: scavenging of active oxygen and dissipation of excess photons. Annu. Rev. Plant Physiol. Plant Mol. Biol., 50, 1: 601-639. ISSN 1040-2519. DOI: 10.1146/annurev.arplant.50.1.601 Go to original source...
    2. BENZIE, I. F. F., STRAIN, J. J., 1996: The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay. Analytical Biochemistry, 239, 1: 70-76. ISSN 1096-0309. DOI: 10.1006/abio.1996.0292 Go to original source...
    3. BRAT, P., GEORGÉ, S., BELLAMY, A., DU CHAFFAUT, L., SCALBERT, A., MENNEN, L., ARNAULT, N., AMIOT, M. A., 2006: Daily Polyphenol Intake in France from Fruit and Vegetables. J. Nutr., 136, 9: 2368-2373. ISSN 1541-6100. DOI: 10.1093/jn/136.9.2368 Go to original source...
    4. BRICIU, A. C., 2010: Study on the influence of Plum pox virus on the chemical composition of leaves and fruits from six varieties of the species Prunus domestica L. Ph.D thesis. Universitatea de Ştiinţe Agricole şi Medicină Veterinară Cluj-Napoca.
    5. BUCHANAN, B. B., GRUISSEM, W., JONES, R. L., 2000: Biochemistry and Molecular Biology of Plants. Rockville, Maryland: American Society of Plant Physiologist, 1189-1203. ISBN 0-943088-39-9.
    6. BYRNE, D. H., NORATTO, G., CISNEROS-ZEVALLOS, L., PORTER, W., VIZZOTTO, M., 2009: Health benefits of peach, nectarine and plums. Acta Hort. (ISHS), 841: 267-274. ISSN 0567-7572. DOI: 10.17660/ActaHortic.2009.841.32 Go to original source...
    7. BYRNE, D., VIZZOTTO. M., 2004: Antioxidant content of peach and plum genotypes. Hort. Sci., 39, 4: 798. ISSN 0018-5345. Go to original source...
    8. CEVALLOS-CASALS, B., BYRNE, D. H., OKIE, W. R., CISNEROS-ZEVALLOS, L., 2006: Selecting new peach and plum genotypes rich in phenolic compounds and enhanced functional properties. Food Chem., 96, 2: 273-280. ISSN 0308-8146. DOI: 10.1016/j.foodchem.2005.02.032 Go to original source...
    9. CHANG, S., TAN, CH., FRANKEL, E. N., BARRETT, D. M., 2000: Low-density lipoprotein antioxidant activity of phenolic compounds and polyphenol oxidase activity in selected clingstone peach cultivars. J. Agric. Food Chem., 48, 2: 147-151. ISSN 0308-8146. DOI: 10.1021/jf9904564 Go to original source...
    10. CHEN, Z., GALLIE, D. R., 2004: The ascorbic acid redox state controls guard cell signaling and stomatal movement. Plant Cell, 16, 5: 1143-1162. ISSN 1532-298X. DOI: 10.1105/tpc.021584 Go to original source...
    11. FOGLIANO, V., VERDE, V., RANDAZZO, G., RITIENI, A., 1999: Method for measuring antioxidant activity and its application to monitoring the antioxidant capacity of wines. J. Agric. Food Chem., 47, 3: 1035-1040. ISSN 0308-8146. DOI: 10.1021/jf980496s Go to original source...
    12. GIL, M. I., TOMÁS-BARBERÁN, F. A., HESS-PIERCE, B., KADER, A. A., 2002: Antioxidant capacities, phenolics compounds, carotenoids, and vitamin C content of nectarine, peach, and plum cultivars from California. J. Agric. Food. Chem., 50, 17: 4976-4982. ISSN 0308-8146. DOI: 10.1021/jf020136b Go to original source...
    13. HELDT, H. W., 2005: Plant Biochemistry. Burlington: Elsevier Academic Press., 105-115. ISBN 0-12-088391-0.
    14. HERNÁNDEZ, A. J., DÍAZ-VIVANCOS, P., RUBIO, M., OLMOS, E., CLEMENTE, J. M., ROS-BARCELÓ, A., MARTÍNEZ-GΌMEZ, P., 2007: Plum pox virus (PPV) infection produces an imbalance on the antioxidative systems in Prunus species. Act. Phytopat. Entomol. Hungar., 42, 2: 209-221. ISSN 1588-2691. DOI: 10.1556/APhyt.42.2007.2.4 Go to original source...
    15. KALOGIROU, M., 2012: Antiviral and quality effects of chemical elicitors and Cucumber Mosaic Virus (CMV) infection on tomato plants and fruits. Ph.D thesis. Cranfield University.
    16. LAMB, C., DIXON, R. A., 1997: The oxidative burst in plant disease resistance. Annu. Rev. Plant Physiol. Plant Mol. Biol., 48, 1: 251-275. ISSN 1040-2519. DOI: 10.1146/annurev.arplant.48.1.251 Go to original source...
    17. MITTLER, R., 2002: Oxidative stress, antioxidants and stress tolerance. Trends in Plant Sci., 7, 9: 405-410. ISSN 1360-1385. DOI: 10.1016/S1360-1385(02)02312-9 Go to original source...
    18. PAREJO, L., CODINA, C., PETRAKIS, C., KEFALAS, P., 2000: Evaluation of scavenging activity assessed by Co(II)/EDTA-induced luminol chemiluminescence and DPPH center dot (2,2-diphenyl-1-picrylhydrazyl) free radical assay. J. Pharmac. Tox. Met., 44, 3: 507-512. ISSN 1056-8719. DOI: 10.1016/S1056-8719(01)00110-1 Go to original source...
    19. POLÁK, J., SALAVA, J. 2008: Metodika hodnocení rezistence meruněk k viru šarky švestky (Plum pox virus). Praha: Výzkumný ústav rostlinné výroby, 22 s. ISBN 978-80-87011-82-9.
    20. RE, R., PELLEGRINI, N., PROTEGGENTE, A., PANNALA, A., YANG, M., RICE-EVANS, C., 1999: Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad. Biol. Med., 26, 9: 1231-1237. ISSN 0891-5849. DOI: 10.1016/S0891-5849(98)00315-3 Go to original source...
    21. ROP, O., JURIKOVA, T., MLCEK, J., KRAMAROVA, D., SENGEE, Z., 2009: Antioxidant activity and selected nutritional values of plums (Prunus domestica L.) typical of the White Carpathian Mountains. Sci. Hort., 122, 4: 545-549. ISSN 0304-4238. DOI: 10.1016/j.scienta.2009.06.036 Go to original source...
    22. ROP, O., MLCEK, J., JURIKOVA, T., VALSIKOVA, M., 2012: Bioactive content and antioxidant capacity of Cape gooseberry fruit. Cent. Eur. J. Biol., 7, 4: 672-679. ISSN 1644-3632. DOI: 10.2478/s11535-012-0063-y Go to original source...
    23. SHALATA, A., MITTOVA, V., VOLOKITA, M. GUY, M., TAL, M., 2001: Response of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii to salt-dependent oxidative stress: The root antioxidative system. Physiol. Plant, 112, 4: 487-494. ISSN 1399-3054. DOI: 10.1034/j.1399-3054.2001.1120405.x Go to original source...
    24. SOCHOR, J., BABULA, P., KRŠKA, B., HORNA, A., PROVAZNÍK, I., 2010a: Evaluation of Output Signals from CoulArray Detector for Determination of Antioxidant Capacity of Apricots Samples. In: Analysis of Biomedical Signals and Images. Analysis of Biomedical Signals and Images. Brno: VUT v Brně, 209-214. ISBN 978-80-214-4106-4.
    25. SOCHOR, J., ZITKA, O., SKUTKOVA, H., PAVLIK, D., BABULA, P., KRSKA, B., HORNA, A., ADAM, V., PROVAZNIK, I., KIZEK, R., 2010b: Content of Phenolic Compounds and Antioxidant Capacity in Fruits of Apricot Genotypes. Molecules, 15, 9: 6285-6305. ISSN 1420-3049. DOI: 10.3390/molecules15096285 Go to original source...
    26. TOMAS-BARBERAN, F. A., GIL, M. I., CREMIN, P., WATERHOUSE, A. L., HESS-PIERCE, B., KADER, A. A. 2001: HPLC-DAD-ESIMS analysis of phenolic compounds in nectarines, peaches and plums. J. Agric. Food. Chem, 49, 10: 4748-4760. ISSN 0308-8146. DOI: 10.1021/jf0104681 Go to original source...
    27. VIZZOTTO, M. 2005: Inhibition of invasive breast cancer cells by selected peach and plum antioxidants. Ph.D thesis. Texas A&M University, College Station.
    28. VIZZOTTO, M., CISNEROS, L., OKIE, W. R., RAMMING, D. W., BYRNE, D. H., 2007: Antioxidant activity and the content of phenolics and anthocyanins in peach and plum. J. Amer. Soc. Hort. Sci., 132, 3: 334-340. ISSN 0003-1062. DOI: 10.21273/JASHS.132.3.334 Go to original source...
    29. VRÁNOVÁ, E., INZÉ, D., VAN BREUSEGEM, F., 2002: Signal transduction during oxidative stress. J. Exp. Bot., 53, 372: 1227-1236. ISSN 1460-2431. DOI: 10.1093/jexbot/53.372.1227 Go to original source...
    30. WINSTON G. W., 1990: Stress Responses in Plants: Adaptation and Acclimation Mechanisms. New York: Wiley-Liss, pp. 407, ISBN 0-471-56810-4.

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