Acta Univ. Agric. Silvic. Mendelianae Brun. 2013, 61(1), 227-232 | DOI: 10.11118/actaun201361010227

Crude protein, fibre and phytic acid in vitro digestibility of selected legume and buckwheat samples

Petra Vojtíšková, Stanislav Kráčmar
Department of Food Analysis and Chemistry, Tomas Bata University in Zlín, nám. T. G. Masaryka 5555, 760 01 Zlín, Czech Republic

The aim of this study was to determine crude protein, fibre and phytic acid in vitro digestibility of selected legumes and buckwheat products. All analyses except the phytic acid contents were performed in the line with the Commission Regulation (EC) No. 152/2009. A modified version of Holt's Method was used for phytic acid (phytate) determination. None of all samples contained more than 11% of moisture. Soybeans are rich in crude protein; they contain nearly 40% of this compound. The content of crude protein in buckwheat flours was about 14%. The highest amount of phytate was found in common beans and soybeans-about 2 g/100 g of dry matter. On the other hand, the lowest phytate content was observed in buckwheat pasta (< 1 g/100 g). The quantification of phytate in F. esculentum groats was 1.9 g per 100 g of dry matter. In vitro digestibility was determined using an incubator Daisy and pepsin enzymes and the combination of pepsin and pancreatin. The highest coefficient of crude protein digestibility was discovered to be in peels and wholemeal flour. The greatest fibre digestibility coefficients were obtained for peels, which contain about 65% of fibre in their dry matter. When pepsin was used, a higher phytic acid digestibility coefficient for G. max, Ph. vulgaris, peels, flour, groats and broken groats was observed; while when the combination of pepsin and pancreatin was used, higher phytic acid digestibility coefficients for peas, lentil and wholemeal flour were observed.

Keywords: legumes, Fagopyrum esculentum Moench, buckwheat products, phytic acid, digestibility
Grants and funding:

This work was kindly supported by a project of the Ministry of Education, Youth and Sports of the Czech Republic (grant No. MSM 70883 52101).

Received: October 26, 2012; Published: April 8, 2013  Show citation

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Vojtíšková, P., & Kráčmar, S. (2013). Crude protein, fibre and phytic acid in vitro digestibility of selected legume and buckwheat samples. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 61(1), 227-232. doi: 10.11118/actaun201361010227
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    References

    1. BLATTNÁ, J., DOSTÁLOVÁ, J., PERLÍN, C., TLÁSKAL, P., 2005: Výživa na začátku 21. století aneb o výživě aktuálně a se zárukou. Praha: Výživaservis, s. r. o., 79 p. ISBN 80-239-6202-7.
    2. BONAFACCIA, G., FABJAN, N., 2003: Nutritional comparison of tartary buckwheat with common buckwheat and minor cereals. Zbiornik Biotehniške Fakultet Univerze v Ljubljani, Vol. 81, No. 2, p. 349-355.
    3. BONAFACCIA, G., MAROCCHINI, M., KREFT, I., 2003: Composition and technological properties of the flour and bran from common buckwheat. Food Chemistry, Vol. 80, p. 9-15. ISSN 0308-8146. DOI: 10.1016/S0308-8146(02)00228-5 Go to original source...
    4. CAMPOS-VEGA, R., LOARCA-PIÑA, G., OOMAH, B. D., 2010: Minor components of pulses and their potential impact on human health. Food Research International, Vol. 43, p. 461-482. ISSN 0963-9969. DOI: 10.1016/j.foodres.2009.09.004 Go to original source...
    5. COMMISSION REGULATION (EC) No. 152/2009 of January 17, 2009 laying down the methods of sampling and analysis for the official control of feed. Official Journal of the European Union. February 26, p. L 54/12-L 54/19. ISSN 1725-2555.
    6. DALGETTY, D. D., BAIK, B. K., 2003: Isolation and characterization of cotyledon fibres from peas, lentils and chickpeas. Cereal Chemistry, Vol. 80, No. 3, p. 310-315. ISSN 0009-0352. DOI: 10.1094/CCHEM.2003.80.3.310 Go to original source...
    7. DAVIES, N. T., REID, H., 1979: An evaluation of the phytate, zinc, copper, iron and manganese contents of, and Zn availability from, soya-based textured-vegetable-protein meat-substitutes or meat-extenders. British Journal of Nutrition, Vol. 41, p. 579-589. ISSN 0007-1145. DOI: 10.1079/BJN19790073 Go to original source...
    8. DE ALMEIDA COSTA, G. E., DA SILVA QUEIROZ-MONICI, K., PISSINI MACHADO REIS, S. M., DE OLIVIERA, A. C., 2006: Chemical composition, dietary fibre and resistant starch contents of raw and cooked pea, common bean, chickpea and legumes. Food Chemistry, Vol. 94, p. 327-330. ISSN 0308-8146. DOI: 10.1016/j.foodchem.2004.11.020 Go to original source...
    9. DUODU, K. G., TAYLOR, J. R. N., BELTON, P. S., HAMAKER, B. R., 2003: Factors affecting sorghum protein digestibility. Journal of Cereal Science, Vol. 38, p. 117-131. ISSN 1095-9963. DOI: 10.1016/S0733-5210(03)00016-X Go to original source...
    10. FREDLUND, K., ISAKSSON, M., ROSSANDER-HULTHÉN, L., ALMGREN, A., SANDBERG, A. S., 2006: Absorption of zinc and retention of calcium: Dose-dependent inhibition by phytate. Journal of Trace Elements in Medical Biology, Vol. 20, p. 49-57. ISSN 0946-672X. DOI: 10.1016/j.jtemb.2006.01.003 Go to original source...
    11. FRIAS, J., DOBLADO, R., ANTEZANA, J. R., VIDAL-VALVERDE, C., 2003: Inositol phosphate degradation by the action of phytase enzyme in legume seeds. Food Chemistry, Vol. 81, p. 233-239. ISSN 0308-8146. DOI: 10.1016/S0308-8146(02)00417-X Go to original source...
    12. HALBRECQ, B., ROMEDENNE, P., LEDENT, J. F., 2005: Evolution of flowering ripening and seed set in buckwheat (Fagopyrum esculentum Moench): quantitative analysis. European Journal of Agronomy, Vol. 23, p. 209-224. ISSN 1161-0301. DOI: 10.1016/j.eja.2004.11.006 Go to original source...
    13. HÍDVÉGI, M. and LÁSZTITY, R., 2002: Phytic Acid Content of Cereals and Legumes and Interaction with Proteins. Periodica Polytechnica Ser. Chemical Engineering, Vol. 46, No. 1-2, p. 59-62. ISSN 1587-3765.
    14. HOLT, R., 1955: Determination of phytate phosphorus. Journal of the Science of Food and Agriculture, Vol. 6, p. 136. ISSN 0022-5142. DOI: 10.1002/jsfa.2740060303 Go to original source...
    15. IKEDA, S., YAMASHITA, Y., 2004: Buckwheat as a dietary source of zinc, copper and manganese. Fagopyrum, Vol. 14, p. 29-34.
    16. IQBAL, A., KHALIL, I. A., ATEEQ, N., KHAN, M. S., 2006: Nutritional quality of important food legumes. Food Chemistry, Vol. 97, p. 331-335. ISSN 0308-8146. DOI: 10.1016/j.foodchem.2005.05.011 Go to original source...
    17. KHATTAB, R. Y., ARNTFIELD, S. D., NYACHOTI, C. M., 2009: Nutritional quality of legume seeds as affected by some physical treatment, Part 1: Protein quality evaluation. Food Science and Technology, Vol. 42, p. 1107-1112. ISSN 0023-6438. Go to original source...
    18. KRÁČMAR, S. a kol., 1981: Výživa hospodářských zvířat. Návody do cvičení. Vydání první. Brno: Vysoká škola zemědělská, 121 p.
    19. LOTT, J. N. A., OCKENDEN, I., RABOY, V., BATTEN, G. D., 2000: Phytic acid and phosphorus in crop seeds and fruits: A global estimate. Seed Science Research, Vol. 10, No. 1, p. 11-33. ISSN 1475-2735. Go to original source...
    20. MIŠURCOVÁ, L., 2008: Nové nutriční aspekty a využití mořských a sladkovodních řas ve výživě člověka. Disertační práce. Univerzita Tomáše Bati ve Zlíně, Zlín, 120 p. MOLINS, R.A., 1991: Phosphates in Food. Boca Raton, Boston: CRC Press, 261 p. ISBN 084934588X.
    21. PAJTÁŠ, M., BÍRO, D., HORNIAKOVÁ, E., BEŇUŠKA, N., ŠIMKO, M., JURÁČEK, M., 2009: Výživa a kŕmenie zvierat: terminologický náučný slovník. Nitra: Slovenská poľnohospodárska univerzita v Nitre, 150 p. ISBN 978-80-552-0185-6.
    22. QIAO, Y., LIN, X., ODLE, J., WHITTAKER, A., VAN KEMPEN, T. A. T. G., 2004: Refining in vitro digestibility assays: Fractionation of digestible and indigestible peptides. Journal of Animal Science, Vol. 82, p. 1669-1677. ISSN 1525-3163. DOI: 10.2527/2004.8261669x Go to original source...
    23. REDDY, N. R., PIERSON, M. D., SATHE, S. K., SALUNKHE, D. K., 1989: Phytates in Cereals and Legumes. Boca Raton, Florida: CRC Press, 160 p. ISBN 0849361087.
    24. SANDBERG, A. S., 2002: Bioavailability of minerals in legumes. British Journal of Nutrition, Vol. 88, No. 3, p. S281-S285. ISSN 1475-2662. DOI: 10.1079/BJN/2002718 Go to original source...
    25. SARWAR-GILANI, S., SEPEHR, E., 2003: Protein Digestibility and Quality in Products Containing Antinutritional Factors Are Adversely Affected by Old Age in Rats. Journal of Nutrition, Vol. 133, No. 1, p. 220-225. ISSN 1541-6100. DOI: 10.1093/jn/133.1.220 Go to original source...
    26. SKRABANJA, V., KREFT, I., GOLOB, T., MODIC, M., IKEDA, S., IKEDA, K., KREFT, S., BONAFACCIA, G., KNAPP, M., KOSMELJ, K., 2004: Nutrient Content in Buckwheat Milling Fractions. Cereal Chemistry, Vol. 81, No. 2, p. 172-176. ISSN 0009-0352. DOI: 10.1094/CCHEM.2004.81.2.172 Go to original source...
    27. SNEDECOR, G. W., COCHRAN, W. G., 1967: Statistical Methods, 6th ed. Iowa: Iowa State University Press, 579p.
    28. TOSH, S. M., YADA, S., 2010: Dietary fibres in pulse seeds and fractions: Characterization, functional attributes and applications. Food Research International, Vol. 43, p. 450-460. ISSN 0963-9969. DOI: 10.1016/j.foodres.2009.09.005 Go to original source...
    29. VOJTÍŠKOVÁ, P., KRÁČMAR, S., HOZA, I., 2010: Content of phytic acid in selected sorts of legumes. Acta Universitatis agriculturae et silviculturae Mendelianae Brunensis, LVIII, No. 1, p. 217-222. ISSN 1211-8516. DOI: 10.11118/actaun201058010217 Go to original source...
    30. WIJNGAARD, H. H., ARENDT, E. K., 2006: Buckwheat. Cereal Chemistry, Vol. 83, No. 4, p. 391-401. ISSN 0009-0352. DOI: 10.1094/CC-83-0391 Go to original source...
    31. ZEMAN, L. a kol., 1995: Katalog krmiv (tabulky výživné hodnoty krmiv). Vydání první. Pohořelice: VÚVZ, 465 p. ISBN 80-901598-3-4.
    32. ZHOU, J. R., ERDMAN, J. W., 1995: Phytic acid in health and disease. Critical Review in Food Science and Nutrition, Vol. 35, p. 459-508. ISSN 1549-7852. DOI: 10.1080/10408399509527712 Go to original source...

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