Acta Univ. Agric. Silvic. Mendelianae Brun. 2013, 61(1), 169-186 | DOI: 10.11118/actaun201361010169

Milk urea analytical result reliability and its methodical possibilities in the Czech Republic

Jan Říha1, Oto Hanuš1, Marta Kleinová2, Pavel Kopunecz2, Petr Roubal1, Aleš Kolář3, Jaroslav Kopecký4
1 Dairy Research Institute, Prague, Ke Dvoru 12a, 160 00 Praha 6 - Vokovice, Czech Republic
2 Czech Moravia Breeders Association, Hradištko 123, 252 09 Hradištko, Czech Republic
3 Bentley Czech s. r. o., Počernická 96, 108 00 Praha 10, Czech Republic
4 Research Institute for Cattle Breeding Rapotín, Výzkumníků 267, 788 13 Vikýřovice, Czech Republic

Control of milk urea concentration (MUC) can be used in diagnosis of the energy-nitrogen metabolism of cows. There are more analytical methods for MUC estimation and there are also discussions about their result reliability. Aim of this work was to obtain information for MUC result reliability improvement. MUC and MUN (milk urea nitrogen) were investigated in 5 milk sample sets and in 7 calibration/comparison experiments. The positions of reference and indirect methods were changed in experiments. There were following analytical methods for MUC or MUN (in mg.100 ml-1): - photometric method (PH, as reference) based on paradimethylaminobenzaldehyde reaction; - method Ureakvant (UK, as reference) based on difference measurement of the electrical conductivity change during ureolysis; - method Chemspec (CH) based on photometrical measurement of ammonia concentration after ureolysis (as reference); - spectroscopic method in mid infrared range of spectrum (FT-MIR; indirect routine method). In all methodical combinations the correlation coefficients (r) varied from 0.8803 to 0.9943 (P < 0.001). In this way all relationships were relevant. The limits of accuracy and precision of FT-MIR are depend on reference method. Therefore, to pay attention to reference methods results is recommended. Both UK and PH could be calibrated to each other with similar parameters. The MUC reference methods (UK and PH) had better values (lower standard deviations) of accuracy (from 1.14 to 1.81 mg.100 ml-1) and comparable values of repeatability (from 0.65 to 1.83 mg.100 ml-1) as compared to FT-MIR MUC or MUN methods (from 1.39 to 5.6 and from 0.76 to 1.92 mg.100 ml-1) in performed experiments.

Keywords: cow, enzyme, spectrophotometry, infrared spectroscopy, conductivity, repeatability, reproducibility, precision, accuracy
Grants and funding:

This work was supported by projects INGO LA 09030 and NAZV KUS QJ1210301.

Received: September 26, 2012; Published: April 8, 2013  Show citation

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Říha, J., Hanuš, O., Kleinová, M., Kopunecz, P., Roubal, P., Kolář, A., & Kopecký, J. (2013). Milk urea analytical result reliability and its methodical possibilities in the Czech Republic. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 61(1), 169-186. doi: 10.11118/actaun201361010169
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