Acta Univ. Agric. Silvic. Mendelianae Brun. 2011, 59(6), 337-342 | DOI: 10.11118/actaun201159060337

Comparison of extractable soil carbon and dissolved organic carbon by their molecular characteristics

Martina Šestauberová, František Novák
Biologické centrum AV ČR, v. v. i., Ústav půdní biologie, Na Sádkách 7, 370 05 České Budějovice, Česká republika

The aim of this study was to compare the molecular characteristics of extractable soil carbon from biotopes of the Rašeliník watershed and dissolved organic carbon (DOC) from Rašeliník creek, by using the 0.1M pyrophosphate, 0.01M CaCl2 and Britton-Robinson buffer as extraction agents. The molecular weight Mr and weight-average molecular weight Mw, determined by the low pressure size exclusion chromatography, increased in the following sequence: humic substances (HS) in CaCl2 < aquatic HS < HS in sodium pyrophosphate ≤ HS in a buffer. Elution curves of all humic substances were characteristic by two peaks with predominant low-molecular fraction. Mr and Mw values of aquatic humic substances were 5.9 and 7.9 kDa, respectively, and proportion of this low-molecular fraction reached 97%. This corresponds to the fact, that the main fraction of HS in surface waters constitute fulvic acids. Using soil extraction in CaCl2 we obtained the fraction of organic carbon similar to the humic substances contain in DOC. Differences in quantity of humic substances extracted from soils among CaCl2 (mean 0.42 ± 0.39), Britton-Robinson buffer (34.9 ± 11.2) and sodium pyrophosphate (293.2 ± 113.4) were statistically significant. The A465/A665 ratio negatively correlated with molecular weight of humic substances.

Keywords: A465/A665 ratio, DOC, humic substances, molecular weight, size exclusion chromatography
Grants and funding:

This work was supported by the project No. 100018967 Cíl 3 - ČR - Sasko "Investigation of possibilities of organic pollutants minimization in sources of drinking water in Ore Mts".

Received: August 3, 2011; Published: March 16, 2014  Show citation

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Šestauberová, M., & Novák, F. (2011). Comparison of extractable soil carbon and dissolved organic carbon by their molecular characteristics. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 59(6), 337-342. doi: 10.11118/actaun201159060337
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