Acta Univ. Agric. Silvic. Mendelianae Brun. 2011, 59(6), 317-328 | DOI: 10.11118/actaun201159060317

Potential of chlorophyll fluorescence and VIS/NIR spectroscopy measurement use for the detection of nitrogen content and disease infection of apple leaves

Václava Spáčilová1, Ivana Šafránková2
1 Agrotest fyto, s. r. o., Havlíčkova 2787/121, 767 01 Kroměříž, Česká republika
2 Ústav pěstování, šlechtění rostlin a rostlinolékařství, Mendelova univerzita v Brně, Zemědělská 1, 613 00 Brno, Česká republika

A possibility of using spectral methods for determining a nutritional status and detecting pathogens in apple-tree cvs. 'Jonagold' and 'Idared' was verified in an orchard and pot experiments in 2007-2010. Treatments differed in the fertilizer or fungicide dose. Leaf samples were collected from the experimental variants to determine nitrogen content and to measure spectral reflectance (spectrophotometer Avantes USB 2000) and chlorophyll fluorescence imaging (FluorCam). Results of the measurements were correlated to leaf analyses for nitrogen content in dry matter. At the same time, a health status (the occurrence of fungal pathogens Venturia inaequalis and Podosphaera leucotricha) was assessed and changes of photochemical efficiency of PSII of infected leaves were evaluated. The parameters providing the best description of differences in the photosynthetic activity of leaves depending on treatments (parameter Fv/Fm and parameter GENTY, known as ΦPSII - effective quantum yield of PSII) were selected. The values of correlation coefficients of Fv/Fm and ΦPSII depending on fertilization treatments were as follows: Fv/Fm: r = -0.4735, p

Keywords: apple tree, spectral reflectance, chlorophyll fluorescence, Venturia inaequalis, Podosphaera leucotricha, nitrogen content
Grants and funding:

The paper was supported by project MSM2532885901.

Received: July 19, 2011; Published: March 16, 2014  Show citation

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Spáčilová, V., & Šafránková, I. (2011). Potential of chlorophyll fluorescence and VIS/NIR spectroscopy measurement use for the detection of nitrogen content and disease infection of apple leaves. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 59(6), 317-328. doi: 10.11118/actaun201159060317
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