Acta Univ. Agric. Silvic. Mendelianae Brun. 2011, 59(6), 181-188 | DOI: 10.11118/actaun201159060181

Assessment of ranges plasma indices in rainbow trout (Oncorhynchus mykiss) reared under conditions of intensive aquaculture

Radovan Kopp1,2, Jan Mareš2, Štěpán Lang2, Tomáš Brabec2, Andrea Ziková3
1 Botanický ústav AV ČR (Centrum pro cyanobakterie a jejich toxiny), Lidická 25/27, 657 20 Brno, Česká republika
2 Ústav zoologie, rybářství, hydrobiology a včelařství, Mendelova univerzita v Brně, Zemědělská 1, 613 00 Brno, Česká republika
3 Department of Ecophysiology and Aquacultur, Müggelseedamm 310, 12587 Berlin, Germany

Plasma parameters in rainbow trout (Oncorhynchus mykiss) from three various trout farms in the Czech Republic were assessed using automated blood plasma analyser. Non-haemolysed serum from the heart of 48 healthy, randomly selected fish (standard length, mean ± SD = 247.3 ± 24.2 mm; body mass, mean ± SD = 262.18 ± 87.28 g) was analysed for the following plasma parameters: alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, creatine kinase, total protein, cholinesterase, amylase, glucose, lactate, albumin, urea, cholesterol, triglycerides, lipase, Ca, Mg, P, Fe, Na, K and Cl. All data were analysed statistically such as normality assessment by means of Kolmogorov-Smirnov test and adequate statistical testing using various parametric and non-parametric tests for each variable. With regard to data distribution, 19 indices out of 23 (aspartate aminotransferase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, total protein, amylase, glucose, lactate, albumin, urea, cholesterol, triglycerides, Ca, Mg, P, Fe, Na, K and Cl) were normally distributed. The indices were affected by handling time and, accordingly to the physical and chemical properties of water. Estimates obtained were compared with previously reported ranges. The blood automated analyser proved to be a valuable and reliable instrument for the estimation of plasma parameters determining normal ranges in rainbow trout.

Keywords: fish, biochemical variables, aquaculture
Grants and funding:

This study was supported by the Research plan No. MSM6215648905 "Biological and technological aspects of sustainability of controlled ecosystems and their adaptability to climate change", National Agency for Agricultural Research'' (grant No. QI91C001) and by the Internal Grant Agency of the Faculty of Agronomy MENDELU (IP12/2010).

Received: May 16, 2011; Published: March 16, 2014  Show citation

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Kopp, R., Mareš, J., Lang, Š., Brabec, T., & Ziková, A. (2011). Assessment of ranges plasma indices in rainbow trout (Oncorhynchus mykiss) reared under conditions of intensive aquaculture. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 59(6), 181-188. doi: 10.11118/actaun201159060181
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