Acta Univ. Agric. Silvic. Mendelianae Brun. 2011, 59(6), 153-160 | DOI: 10.11118/actaun201159060153

In vitro testing of defense reactions in zygotic and somatic embryos of Abies numidica

Jiří Hřib1, Václav Adamec2, Božena Vooková3
1 Ukrajinská 17, 625 00 Brno, Česká republika
2 Ústav statistiky a operačního výzkumu, Mendelova univerzita v Brně, Zemědělská 1, 613 00 Brno, Česká republika
3 Ústav genetiky a biotechnológií rastlín, Akademie vied SR, Akademická 2, P.O. BOX 39A, 950 07 Nitra, Slovenská republika

Defense of desiccated cotyledonary somatic embryos and mature zygotic embryos of Abies numidica was tested in vitro by dual cultures with tester, fungus Phaeolus schweinitzii. Both types of embryos expressed defense reactions manifested by inhibited growth of fungal tester towards the embryos. Mycelial growth was described by logistic sigmoid growth model with a single asymptote. Mutual comparisons of mycelial growth in presence of zygotic and somatic embryos showed significant differences in parameters of mycelium growth curves towards the embryos. Larger defense reactions were observed in zygotic embryos relative to somatic embryos and unlimited control cultivations without embryo. The possible role of auxin in the defense response of plant embryos is discussed.

Keywords: algerian fir, somatic and zygotic embryos, mycelial growth, Phaeolus schweinitzii, dual cultures

Received: April 19, 2011; Published: March 16, 2014  Show citation

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Hřib, J., Adamec, V., & Vooková, B. (2011). In vitro testing of defense reactions in zygotic and somatic embryos of Abies numidica. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 59(6), 153-160. doi: 10.11118/actaun201159060153
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