Acta Univ. Agric. Silvic. Mendelianae Brun. 2011, 59(3), 209-214 | DOI: 10.11118/actaun201159030209

Occurrence and antimicrobial sensitivity in staphylococci isolated from goat, sheep and cow's milk

Marcela Vyletělová1, Oto Hanuą2, Renáta Karpíąková3, Zora ©»ástková3
1 Výzkumný ústav pro chov skotu a Agrovýzkum Rapotín, s.r.o., Výzkumníků 267, 788 13 Vikýřovice, Česká republika
2 Výzkumný ústav pro chov skotu, Výzkumníků 267, 788 13 Vikýřovice, Česká republika
3 Veterinární a farmaceutická univerzita Brno, Palackého 1-3, 612 42 Brno, Česká republika

The aim of this study was to compare the sensitivity to selected antibiotics in staphylococci isolated from goat (n = 60), sheep (n = 60) and cow's milk (n = 120). The individual milk samples were inoculated onto Blood agar cultivated at 36 °C/24 h.The isolated species of staphylococci were identified using biochemical tests, namely STAPHYtest and identification program TNW pro 6.5. We examined the sensitivity of strains to the spectrum of antibiotics, as follows: vancomycin (VA), amoxicillin/clavulanic acid (AMC), penicillin (P), rifampicin (RD), oxacillin (OX), tetracycline (TE), erythromycin (E), chloramphenicol (C), clindamycin (DA), gentamicin (CN), ciprofloxacin (CIP), teicoplanin (TEC), cefoxitin (FOX) and novobiocin (NOV). Altogether, 97 staphylococcal isolates were obtained; 70 from cow's milk, 11 from goat's milk and 16 from sheep' milk. Staphylococcus aureus was the most frequent species in milk of all animal origin tested, was detected in 54 (45%) cow's milk, 10 (17%) goat's and 15 (25%) sheep' milk samples. S. lentus was identified only in goat's and sheep' milk whereas in cow's milk there were representation of staphylococcal species as follows: S. haemolyticus (n = 7), S. chromogenes (n = 2), S. warneri (n = 2), S. xylosus (n = 2), S. epidermidis (n = 2) and unclassified staphylococci (n = 1). The results of S. aureus sensitivity are similar for all tested antibiotics and for all monitored milk: No resistance to vancomycin, rifampicin, chloramphenicol and teicoplanin was recorded in obtained S. aureus isolates whereas the resistance to ciprofloxacin was found out most often.

Keywords: milk, Staphylococcus spp., antibiotics
Grants and funding:

This work was supported by Ministry of Agriculture project NAZV QH81111 and Ministry of Education projects MSM 2678846201, INGO LA 333, LA 10030 and LA 331.

Received: November 18, 2010; Published: June 14, 2014  Show citation

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Vyletělová, M., Hanuą, O., Karpíąková, R., & ©»ástková, Z. (2011). Occurrence and antimicrobial sensitivity in staphylococci isolated from goat, sheep and cow's milk. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 59(3), 209-214. doi: 10.11118/actaun201159030209
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