Acta Univ. Agric. Silvic. Mendelianae Brun. 2011, 59(3), 51-54 | DOI: 10.11118/actaun201159030051

Extraction of acid phosphomonoesterase from soil: testing of various extractants

Ladislav Holík1, Peter Dundek1, Valerie Vranová1, Klement Rejšek1, Dalibor Janouš2, Pavel Formánek1
1 Ústav geologie a pedologie, Mendelova univerzita v Brně, Zemědělská 3, 613 00 Brno, Česká republika
2 Ústav systémové biologie a ekologie, Poříčí 3b, 603 00 Brno, Česká republika

The aim of this work was to to investigate the suitability of various types of extractants for extraction of acid phosphomonoesterase from soil using various types of extractants. Succinate-borate buffer of pH 4.8 or 0.1 M K2SO4 were the most efficient to extract this enzyme compared to 0.1 M glutamic acid or 0.005 M salicylic acid. Extraction using 0.1 M glutamic acid gave significantly (P

Keywords: acid phosphomonoesterase, extracts, glutamic acid, K2SO4, salicylic acid, soil
Grants and funding:

The study was supported by the grant MSM6215648902 / Forest and Wood: the support of functionally integrated forest management and use of wood as a renewable raw material phase 4/2/3, part II "The management strategy of nature conservation areas", and the project "Activity of acid phosphomonoesterase in soil of mountain ecosystems: elaboration of the methods and their application" (Complementary activities of the Mendel University in Brno).

Received: January 13, 2011; Published: June 14, 2014  Show citation

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Holík, L., Dundek, P., Vranová, V., Rejšek, K., Janouš, D., & Formánek, P. (2011). Extraction of acid phosphomonoesterase from soil: testing of various extractants. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 59(3), 51-54. doi: 10.11118/actaun201159030051
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