Acta Univ. Agric. Silvic. Mendelianae Brun. 2011, 59(3), 29-34 | DOI: 10.11118/actaun201159030029

The effect of amino acid enantiomers on activity of selected enzymes in soil

Peter Dundek, Ladislav Holík, Valerie Vranová, Klement Rejšek, Pavel Formánek
Ústav geologie a pedologie, Mendelova univerzita v Brně, Zemědělská 3, 613 00 Brno, Česká republika

This work was aimed to test the effect of selected amino acid enantiomers on activity of casein-protease and acid phosphomonoesterase in soil. Casein-protease was selected due to its key role in nitrogen mineralization and acid phosphomonoesterase due to its importance in soil organic P mineralization. The results showed that 5 mg of L- and D-glutamic acid added to fresh soil from Ah horizon of a moderately mown mountain meadow significantly (P < 0.05) decreased casein-protease activity, whereas alanine enantiomers slightly increased activity of this enzyme. Testing the effect of cystine on activity of acid phosphomonoesterase in soil showed slight increase of this activity after application of 3.2 mg L- or D-cystine to fresh soil (equivalent to 8 mg to dry soil).

Keywords: glutamic acid, alanine, cystine, enantiomers, soil, protease, phosphomonoesterase
Grants and funding:

The study was supported by the grant MSM6215648902 / Forest and Wood: the support of functionally integrated forest management and use of wood as a renewable raw material phase 4/2/2, part II "The management strategy of nature conservation areas", and by project "Extension of current knowledge on bioavailable amino acids in soil and their utilization by soil microorganisms and plant roots"(IGA FFWT MENDELU No. 47/2010-2012).

Received: January 13, 2011; Published: June 14, 2014  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Dundek, P., Holík, L., Vranová, V., Rejšek, K., & Formánek, P. (2011). The effect of amino acid enantiomers on activity of selected enzymes in soil. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis59(3), 29-34. doi: 10.11118/actaun201159030029
Download citation

References

  1. AMELUNG, W., BRODOWSKI, S., 2002: In vitro quantification of hydrolysis-induced racemization of amino acid enantiomers in environmental samples using deuterium labeling and electron-impact ionization mass spectrometry. Analytical Chemistry 74, 13, 3239-3246. ISSN 0003-2700. DOI: 10.1021/ac011022m Go to original source...
  2. AMELUNG, W., ZHANG, X., 2001: Determination of amino acid enantiomers in soils. Soil Biology and Biochemistry 33, 4-5: 553-562. ISSN 0038-0717. DOI: 10.1016/S0038-0717(00)00195-4 Go to original source...
  3. AMELUNG, W., ZHANG, X., FLACH, K. W., 2006: Amino acids in grassland soils: Climatic effect on concentrations and chirality. Geoderma 130, 3-4: 207-217. ISSN 0016-7061. DOI: 10.1016/j.geoderma.2005.01.017 Go to original source...
  4. ASMAR, F., EILAND, F., NIELSEN, N. E., 1994: Effect of extracellular-enzyme activities on solubilization rate of soil organic nitrogen. Biology and Fertility of Soil 17, 1: 32-38. ISSN 0178-2762. DOI: 10.1007/BF00418669 Go to original source...
  5. BRÜCKNER, H., WASTHAUSER, T., 2003: Chromatographic determination of L- and D- amino acids in plants. Amino Acids 24, 2: 43-55. ISSN 1948-5948. DOI: 10.1007/s00726-002-0401-x Go to original source...
  6. CARREIRA, J. A., GARCA-RUIZ, R., LIÉTOR, J., HARRISON, A. F., 2000: Changes in soil phosphatase activity and P transformation rates induced by application of N- and S-containing acid-mist to a forest canopy. Soil Biology and Biochemistry 32, 13: 1857-1865. ISSN 0038-0717. DOI: 10.1016/S0038-0717(00)00159-0 Go to original source...
  7. FORMÁNEK, P., KLEJDUS, B., VRANOVÁ, V., 2005: Bio-available amino acids extraction from soil by demineralized water and 0.5 M ammonium acetate. Amino Acids 28, 4: 427-429. ISSN 0939-4451. DOI: 10.1007/s00726-005-0190-0 Go to original source...
  8. FORMÁNEK, P., REJŠEK, K., VRANOVÁ, V., MAREK, M. V., 2008a: Bio-available amino acids and mineral nitrogen forms in soil of moderately mown and abandoned mountain meadows. Amino Acids 34, 2: 301-306. ISSN 0939-4451. DOI: 10.1007/s00726-006-0468-x Go to original source...
  9. FORMÁNEK, P., REJŠEK, K., VRANOVÁ, V., KLEJDUS, B., 2008b: Selected Diamino Acids in Soils of Differently Managed Mountain Meadow and Forest Ecosystems: Assessment of their Role in the Ecosystem Nutrition. In: SCHÄFER, H. A., WOHLBIER, L. M.: Diamino Amino Acids. 1. vyd. New York: Nova Science Publishers, 182-218. ISBN 978-1-60456-892-9.
  10. GLASER, B., AMELUNG, W., 2002: Determination of 13C natural abundance of amino acid enantiomers in soil: methodological considerations and first results. Rappid Communications in Mass Spectrometry 16, 9: 891-898. ISSN 0951-4198. DOI: 10.1002/rcm.650 Go to original source...
  11. GLENN, A. R., 1976: Production of extracellular proteins by bacteria. Annual Review of Microbiology 30: 41-62. ISSN 0066-4227. DOI: 10.1146/annurev.mi.30.100176.000353 Go to original source...
  12. HOPKINS, D. W., FERGUSON, K. E., 1994: Substrate induced respiration in soil amended with different amino acid isomers. Applied Soil Ecology 1, 1: 75-81. ISSN 0929-1393. DOI: 10.1016/0929-1393(94)90025-6 Go to original source...
  13. HOPKINS, D. W., ISABELLA, B. L., SCOTT, S. E., 1994: Relationship between microbial biomass and substrate induced respiration in soils amended with D- and L-isomers of amino acids. Soil Biology and Biochemistry 26, 12: 1623-1627. ISSN 0038-0717. DOI: 10.1016/0038-0717(94)90314-X Go to original source...
  14. HOPKINS, D. W., O'DOWD, R. W., SHIELD, R. S., 1997: Comparison of D- and L-amino acid metabolism in soils with differing microbial biomass and activity. Soil Biology and Biochemistry 29, 1: 23-29. ISSN 0038-0717. DOI: 10.1016/S0038-0717(96)00266-0 Go to original source...
  15. LANDI, L., RENELLA, G., MORENO, J. L., FALCHINI, F. L., NANNIPIERI, P., 2000: Influence of cadmium on the metabolic quotient, L-D-glutamic acid respiration ratio and enzyme activity: microbial biomass ratio under laboratory conditions. Biology and Fertility of Soils 32, 1: 8-16. ISSN 0178-2762. DOI: 10.1007/s003740000205 Go to original source...
  16. MARTENS, D. A., FRANKENBERGER W. T. JR., 1993: Metabolism of tryptophan in soil. Soil Biology and Biochemistry 25, 12: 1679-1687. ISSN 0038-0717. DOI: 10.1016/0038-0717(93)90170-G Go to original source...
  17. NOWAK, J., KAKLEWSKI, K., KLÓDKA, D., 2006: Influence of various concentrations of selenic acid (IV) on the activity of soil enzymes. Science of the Total Environment 291, 1-3: 105-110. ISSN 0048-9697. DOI: 10.1016/S0048-9697(01)01072-5 Go to original source...
  18. O'DOWD, R. W., HOPKINS, D. W., 1998: Mineralization of carbon from D- and L-amino acids and D-glucose in two contrasting soils. Soil Biology and Biochemistry 30, 14: 2009-2016. ISSN 0038-0717. DOI: 10.1016/S0038-0717(98)00075-3 Go to original source...
  19. O'DOWD, R. W., PARSONS, R., HOPKINS, D. W., 1997: Soil respiration induced by the D- and L-isomers of a range of amino acids. Soil Biology and Biochemistry 29, 11: 1665-1671. ISSN 0038-0717. DOI: 10.1016/S0038-0717(97)00080-1 Go to original source...
  20. O'DOWD, R. W., BARRACLOUGH, D., HOPKINS, D. W., 1999: Nitrogen and carbon mineralization in soil amended with D- and L- leucine. Soil Biology and Biochemistry 31, 11: 1573-1578. ISSN 0038-0717. DOI: 10.1016/S0038-0717(99)00083-8 Go to original source...
  21. REJŠEK, K., 1991: Acid phosphomonoesters activity of ectomycorrhizal roots in Norway spruce pure stands exposed to pollution. Soil Biology and Biochemistry 23, 7: 667-671. ISSN 0038-0717. DOI: 10.1016/0038-0717(91)90081-T Go to original source...
  22. REJŠEK, K., FORMÁNEK, P., PAVELKA, M., 2008: Estimation of protease activity in soils at low temperatures by casein amendment and with substitution of buffer by demineralized water. Amino Acids 35, 2: 411-417. ISSN 0939-4451. DOI: 10.1007/s00726-007-0601-5 Go to original source...
  23. REJŠEK, K., FORMÁNEK, P., VRANOVÁ, V., 2010: The Soil Amino Acids: Quality, Distribution and Site Ecology. 1. vyd. New York: Nova Science Publishers, Inc. 222 s. ISBN 978-1-61668-335-1.
  24. TRASAR-CEPEDA, C., LEIRÓS, M. C., GIL-SOTRES, F., 2000: Biochemical properties of acid soils under climax vegetation (Atlantic oakwood) in an area of the European temperate-humid zone (Galicia, NW Spain): Specific parameters. Soil Biology and Biochemistry 32, 6: 747-755. ISSN 0038-0717. DOI: 10.1016/S0038-0717(99)00196-0 Go to original source...
  25. VÁGNEROVÁ, K., MACURA, J., 1974: Relationship between plant roots, proteolytic organisms and activity of protease. Folia Microbiologica 19, 6: 525-535. ISSN 0015-5632. DOI: 10.1007/BF02872920 Go to original source...
  26. VOET, D., VOET, J. G., 1995: Biochemistry. John Wiley & Sons Ltd, New York. ISBN 0-471-19350-X.
  27. VRANOVÁ, V., FORMÁNEK, P., REJŠEK, K., PAVELKA, M., 2009: Impact of land-use change on proteolytic activity of mountain meadows. Soil and Water Research 3, 4: 122-125. ISSN 1801-5395. DOI: 10.17221/16/2009-SWR Go to original source...

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY NC ND 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.