Acta Univ. Agric. Silvic. Mendelianae Brun. 2011, 59(1), 129-136 | DOI: 10.11118/actaun201159010129

Comparison of antibiotic resistance between ecological and conventional breeding in Enterobacteriaceae genera isolated from milk and milk products

Lukáš Hleba1, Miroslava Kačániová1, Simona Kunová2, Maciej Kluz3, Martin Mellen4
1 Katedra mikrobiológie, Slovenská poľnohospodárska univerzita v Nitre, 949 76 Nitra, Slovenská republika
2 Katedra hygieny a bezpečnosti potravín, Slovenská poľnohospodárska univerzita v Nitre, 949 76 Nitra, Slovenská republika
3 Katedra biotechnologii i mikrobiologii, Wydzial biologiczno-rolniczy, Ul. Cwiklinskiej 2, 35-601 Rzeszow, Poland
4 Ministerstvo pôdohospodárstva SR, Dobrovičova 12, 812 66 Bratislava, Slovenská republika

The aim of this study was to determine and compare antibiotic resistance of Enterobacteriaceae genera isolated from milk and milk products like cheese, bryndza, srvátka and parenica from ecological and conventional breeding from different regions of Slovakia. Distance between breedings was about 20 km. In the both breeding were not used antibiotics. The Enterobacteriaceae isolates were tested for susceptibility to three antibiotics ampicillin (AMP), nalixid acid (NA 30) and chloramphenicol (C 30). In our study, we determined that the highest resistance of Enterobacteriaceae strains was to ampicillin (100 %) in conventional breeding. The higher resistance in conventional breeding is probably due to greater anthropogenic influences. In ecological breeding we determined 84.61% resistance to ampicillin. The highest susceptibility was to chloramphenicol and nalixid acid (100 %) in conventional breeding. In the ecological breeding resistance to chloramphenicol was 15.38 % and resistance to nalixid acid 7.69 % from all tested isolates. Conversely, higher resistance to chloramphenicol and nalixid acid in the ecological breeding may be due to the persistence of resistant genes in environment. The lowest resistance to chloramphenicol and nalixid acid in conventional breeding may be due to the prohibition of the use of antibiotics as growth promoters. From this genera, we identified Klebsiella oxytoca, Serratia odorifera bv.1, Serratia odorifera bv. 2, Citrobacter braakii and Escherichia coli from conventional breeding which were resistant to ampicillin and isolated from cheese, bryndza and parenica. In the ecological breeding we identified this strains: Raoultella ornithinolytica resistant to ampicillin, Serratia rubidaea, which was resistant to all of used antibiotics and this strains were isolated from milk samples and Klebsiella pneumoniae, Escherichia coli, Klebsiella oxytoca which were resistant to ampicillin and were isolated from cheese samples. The results show that the bacteria can transfer resistance genes to others bacteria, for example to pathogens too. Also that resistant bacteria and their resistant genes survive in the environment and transfer to others animals and products thereof.

Keywords: antibiotic resistance, Enterobacteriaceae, milk and milk products, ecological and conventional breeding
Grants and funding:

This work has been supported by grant of VEGA 1/0372/09 and KEGA 430-014SPU-4/2010.

Received: August 20, 2010; Published: July 10, 2014  Show citation

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Hleba, L., Kačániová, M., Kunová, S., Kluz, M., & Mellen, M. (2011). Comparison of antibiotic resistance between ecological and conventional breeding in Enterobacteriaceae genera isolated from milk and milk products. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 59(1), 129-136. doi: 10.11118/actaun201159010129
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