Acta Univ. Agric. Silvic. Mendelianae Brun. 2010, 58(1), 217-222 | DOI: 10.11118/actaun201058010217

Obsah kyseliny fytové ve vybraných druzích luštěnin

Petra Vojtíšková, Stanislav Kráčmar, Ignác Hoza
Univerzita Tomáše Bati ve Zlíně, Fakulta technologická, Ústav biochemie a analýzy potravin, nám. T. G. Masaryka 275, 762 72 Zlín, Česká republika

V práci byl sledován obsah kyseliny fytové (fytátu) ve vybraných druzích luštěnin, sójových bobů (Glycine max), žlutého loupaného hrachu (Pisum sativum) a čočky (Lens esculenta). Zkoumané vzorky byly homogenizovány. Vlhkost vzorků byla stanovena podle Nařízení komise (ES) č. 152/2009 uvedeného v Úředním věstníku Evropské Unie. Vlhkost sójových bobů byla 8,42 %, žlutého loupaného hrachu 11,19 % a čočky 10,07 %. Obsah kyseliny fytové byl stanoven modifikovanou metodou podle Holta jako množství fytátu. Standardní křivka byla proměřena pomocí 0,2mM standardního roztoku fytátu sodného v rozmezí koncentrací 40 až 200 nmol. Obsah fytátu v sójových bobech (G. max) kolísal mezi 1,28 až 1,86 % v sušině a mezi 1,17 až 1,70 g ve 100 g vzorku, ve žlutém loupaném hrachu (P. sativum) v rozmezí od 0,49 do 0,86 % v sušině a od 0,43 do 0,77 g ve 100 g vzorku a u čočky (L. esculenta) v rozmezí 0,45-1,39 % v sušině a 0,40-1,25 g ve 100 g vzorku.

kyselina fytová, fytát, Glycine max, Pisum sativum, Lens esculenta

Content of phytic acid in selected sorts of legumes

The aim of this study was to determine the content of phytic acid (phytate) in soybeans (Glycine max), yellow shelled peas (Pisum sativum) and lentil (Lens esculenta). Dry seeds were grounded to a fine powder. The moisture of samples was determined according to the Official Journal of the European Union (2009). The moisture of the samples was 8.42% in soybeans, 11.19% in yellow shelled peas and 10.07% in lentil. The content of phytic acid was determined by the modified Holt's method (1955) using the spectrophotometer. Standard curve was measured using the Na phytate standard solution (0.2 mM). The phytate content in G. max varied from 1.28 to 1.86% in dry matter and from 1.17 to 1.70 g per 100 g of the sample. The content of phytate in P. sativum ranged from 0.49 to 0.86% and from 0.43 to 0.77 g per 100 g of the sample. The phytate content in L. esculenta varied from 0.45 to 1.39% in dry matter and from 0.40 to 1.25 g per 100 g of the sample. These obtained values could be influenced by many factors, e.g. climatic conditions, location, variety, etc.

Keywords: phytic acid, phytate, Glycine max, Pisum sativum, Lens esculenta
Grants and funding:

This work was kindly supported by a project of the Ministry of Education, Youth and Sports of the Czech Republic (grant No. MSM 70883 52101).

Received: October 7, 2009; Published: October 5, 2014  Show citation

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Vojtíšková, P., Kráčmar, S., & Hoza, I. (2010). Content of phytic acid in selected sorts of legumes. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 58(1), 217-222. doi: 10.11118/actaun201058010217
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