Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(5), 243-250 | DOI: 10.11118/actaun200957050243

Dědičnost rezistence meruněk k viru šarky švestky u potomstev odrůdy 'Harlayne'

Petra Pilařová, Boris Krška
Ústav ovocnictví, Mendelova zemědělská a lesnická univerzita v Brně, Valtická 337, 691 44 Lednice, Česká republika

Cílem této práce bylo určit počet genů rozhodujících o dědičnosti rezistence meruněk k šarce švestek, které by mohly být využity v dalších šlechtitelských plánech. Za tímto účelem byla vybrána čtyři potomstva, u nichž byla odrůda 'Harlayne' použita jako zdroj rezistence k tomuto viru. Jedinci těchto potomstev byli inokulováni kmeny PPV-M a PPV-D.
Pozorování symptomů na listech bylo prováděno minimálně pětkrát za vegetační sezonu. Pro potvrzení přítomnosti nebo absence viru šarky švestky v rostlinách byl použit DAS-ELISA test, který byl prováděn dvakrát během vegetační sezony. Přítomnost infekce viru šarky švestky byla hodnocena po dobu tří nebo pěti vegetačních sezon. Na základě získaných výsledků během pozorování a testování byli jedinci rozděleni na rezistentní a citlivé. Za citlivé byli považování ti, na jejichž listech se během doby hodnocení ukázaly příznaky šarky a/nebo výsledky ELISA testu byly pozitivní.
První mírné příznaky šarky se objevily již v roce inokulace, ale síla příznaků byla mnohem větší po první dormanci. Počet citlivých jedinců přibýval každý rok a to i pátým rokem po inokulaci. Závislost přítomnosti symptomů a pozitivního výsledku ELISA testu byla vysoká.
Po třech nebo pěti letech hodnocení byl vypočítán poměr mezi rezistentními a citlivými jedinci v jednotlivých populacích. V populacích byl poměr rezistentní: citlivý po třech letech hodnocení následující 'Harlayne' × 'Strepet' 39:61, 'Strepet' × 'Harlayne' 53:47, 'Orangered' × 'Harlayne' 47:53, v případě populace 'Harlayne' × 'Vestar' po pěti letech 23:77 pro kmen PPV-M a 29:71 pro kmen PPV-D. Tyto poměry byly porovnány pomocí χ2-testu s teoretickými. Ze získaných výsledků čtyř potomstev nelze jednoznačně určit, zda rezistenci k šarce švestky kontrolují dva (p = 0.8 a p = 0.69, nejvyšší hodnoty p u potomstva 'Harlayne' × 'Strepet' a 'Harlayne' × 'Vestar', PPV-D) nebo tři (p = 0.91 a p = 0.43, nejvyšší hodnoty p u potomstva 'Harlayne' × 'Vestar', PPV-D a 'Orangered' × 'Harlayne') dominantní geny.

PPV, šarka švestky, Prunus armeniaca L., štěpný poměr, šlechtění

Inheritance of resistance to Plum pox virus in the progeny of the apricot cv. 'Harlayne'

Natural resistance to Plum pox virus (PPV), the agent of sharka disease, is one of the most important traits of interest to stone fruit breeders, although few sources of resistance have been identified. One of the few apricot cultivars which does show resistance, 'Harlayne', was chosen for a study of the genetics of PPV resistance. It was crossed with three different cultivars, two susceptible ('Vestar' and 'Strepet') and one immune ('Orangered'). Four different lines (since there was one reciprocal combination) were established and the F1 crosses were subsequently inoculated with the PPV-M and PPV-D strains by grafting infected buds. A woody indicator Prunus persica 'GF 305' was then also top-grafted onto the plants of three of these F1 populations. The observations of leaf symptoms and accompanying ELISA tests were performed over three, or in one case five, growing seasons and then hybrids were classified accordingly, as either resistant or susceptible. The resistant : susceptible ratios were calculated and compared with expected theoretical ratios using the χ2-test. The ratios of resistant to susceptible plants in the progeny derived from the four apricot crosses are compatible with the hypothesis of three dominant genes being responsible for PPV resistance, with 'Harlayne' being heterozygous for all three genes. However, the possibility that resistance is controlled by just two dominant genes can not be ruled out just yet.

Keywords: PPV, sharka disease, Prunus armeniaca L., segregation ratio, breeding
Grants and funding:

This work was partly supported by project 9/2008/591 from the Grant Agency of Mendel University of Agriculture and Forestry and by the SharCo FP7 Small collaborative research project, number 204429.

Received: February 18, 2009; Published: October 10, 2014  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Pilařová, P., & Krška, B. (2009). Inheritance of resistance to Plum pox virus in the progeny of the apricot cv. 'Harlayne'. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 57(5), 243-250. doi: 10.11118/actaun200957050243
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. AMENDUNI, T., BAZZONI, A., MINAFRA, A. and SAVINO, V., 2004: Evaluation of the susceptibility of seedlings from apricot crosses to the Marcus strain of plum pox virus. Acta Hort, 657: 305-308. ISSN 0567-7572 DOI: 10.17660/ActaHortic.2004.657.47 Go to original source...
    2. Atanassoff, D., 1932: Plum pox: a new virus disease. Yearbook University of Sofia, Faculty of Agriculture, 11: 49-60 In: LÓPEZ-MOYA, J. J., FERNÁNDEZ-FERNÁNDEZ, M. R., CAMBRA, M. and GARCÍA, J. A., 2000: Biotechnological aspects of plum pox virus. Journal of Biotechnology, 76: 121-136. ISSN 0168-1656 DOI: 10.1016/S0168-1656(99)00196-0 Go to original source...
    3. Cambra, M., Capote, N., Myrta, A. and Llácer, G., 2006: Plum pox virus and the estimated costs associated with sharka disease. EPPO Bull, 36: 202-204. ISSN 1365-2338 DOI: 10.1111/j.1365-2338.2006.01027.x Go to original source...
    4. Candresse, T. and Cambra, M., 2006: Causal agent of sharka disease: historical perspective and current status of Plum pox virus strains. EPPO Bull, 36: 239-246. ISSN 1365-2338 DOI: 10.1111/j.1365-2338.2006.00980.x Go to original source...
    5. Clark, M. F. and Adams, A. N., 1977: Characteristics of the micro-plate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol, 34, 3: 475-483. ISSN 1465-2099 DOI: 10.1099/0022-1317-34-3-475 Go to original source...
    6. Dicenta, F., Martínez-Gómez, P., Burgos, L. and Egea, J., 2000: Inheritance of resistance to Plum pox potyvirus (PPV) in apricot, Prunus armeniaca. Plant Breed, 119: 161-164. ISSN 1439-0523 DOI: 10.1046/j.1439-0523.2000.00458.x Go to original source...
    7. Dosba, F., Denise, F., Audergon, J. M., Maison, P. and Massonie, G., 1991: Plum pox virus resistance of apricot. Acta Hort, 293: 569-579. ISSN 0567-7572 DOI: 10.17660/ActaHortic.1991.293.69 Go to original source...
    8. DOSBA, F., ORLIAC, S., DUTRANNOY, F., MAISON, P., MASSONIÉ, G. and AUDERGON, J. M., 1992: Evaluation of resistance to plum pox virus in apricot trees. Acta Hort, 309: 211-220. ISSN 0567-7572 DOI: 10.17660/ActaHortic.1992.309.30 Go to original source...
    9. FUCHS, E., GRÜNTZIG, M. and ERNST, I., 2001: Comparison of apricot genotypes with different resistence level to plum pox virus (PPV). Acta Hort, 550: 103-106. ISSN 0567-7572 DOI: 10.17660/ActaHortic.2001.550.12 Go to original source...
    10. Gadiou, S., Šafářová, D. and Navrátil, M., 2008: Genetic variability of Plum pox virus isolates in the Czech Republic. Eur J Plant Pathol, (in press). doi: 10.1007/s10658-008-9272-7 DOI: 10.1007/s10658-008-9272-7 Go to original source...
    11. Guillet-Bellanger, I. and Audergon, J. M., 2001: Inheritance of the Stark Early Orange apricot cultivar resistance to Plum pox virus. Acta Hort, 550: 111-116. ISSN 0567-7572 DOI: 10.17660/ActaHortic.2001.550.14 Go to original source...
    12. ION-NAGY, L., LANSAC, M., EYQUARD, J. P., SALVADOR, B., GARCIA, J. A., LE GALL, O., HERNOULD, M., SCHURDI-LEVRAUD, V. and DECROOCQ, V., 2006: PPV long-distance movement is occasionally permitted in resistant apricot hosts. Virus Research, 120: 70-78. ISSN 0168-1702 DOI: 10.1016/j.virusres.2006.01.019 Go to original source...
    13. Karayiannis, I., Thomidis, T. and Tsaftaris, A., 2008: Inheritance of resistance to Plum pox virus in apricot (Prunus armeniaca L.). Tree Genetics & Genomes, 4: 143-148. ISSN 1614-2942 DOI: 10.1007/s11295-007-0095-z Go to original source...
    14. KRŠKA, B., SALAVA, J. and POLÁK, J., 2006 a: Breeding for Plum pox virus resistant apricots (Prunus armeniaca L.) in the Czech Republic. EPPO Bull, 36: 330-331. ISSN 1365-2338 DOI: 10.1111/j.1365-2338.2006.01009.x Go to original source...
    15. Krška, B., Salava, J., Polák, J. and Komínek, P., 2002: Genetics of resistance to plum pox virus in apricot. Plant Prot Sci, 38: 180-182. ISSN 1212-2580 DOI: 10.17221/10350-PPS Go to original source...
    16. KRŠKA, B., VACHŮN, Z. and NEČAS, T., 2006 b: The apricot breeding programme at the Horticulture Faculty in Lednice. Acta Hort, 717: 145-146 ISSN 0567-7572 DOI: 10.17660/ActaHortic.2006.717.29 Go to original source...
    17. Martínez-Gómez, P. and Dicenta, F., 2000: Evaluation of resistance of apricot cultivars to a Spanish isolate of plum pox potyvirus (PPV). Plant Breed, 119: 179-181. ISSN 1439-0523 DOI: 10.1046/j.1439-0523.2000.00457.x Go to original source...
    18. Martínez-Gómez, P., Dicenta, F. and Egea, J., 2003: Effect of a traditional control method (tree removal) on the spread of sharka in an apricot orchard in Southeastern Spain. Phytopathol. Mediterr, 42: 161-166. ISSN 0031-9465
    19. MARTÍNEZ-GÓMEZ, P., RUBIO, M. and DICENTA, F., 2003: Evaluation of resistance to plum pox virus of North American and European apricot cultivars. HortScience, 38: 568-569. ISSN: 0018-5345 DOI: 10.21273/HORTSCI.38.4.568 Go to original source...
    20. Moustafa, T. A., Badenes, M. L., Martínez-Calvo, J. and Llácer, G., 2001 b: Studies on Plum pox (sharka) resistance in apricot. Acta Hort, 550: 117-120. ISSN 0567-7572 DOI: 10.17660/ActaHortic.2001.550.15 Go to original source...
    21. MOUSTAFA, T. A., BADENES, M. L., MARTÍNEZ-CALVO, J. and LLÁCER, G., 2001 a: Determination of resistance to sharka (plum pox) virus in apricot. Scientia Hort, 91: 59-70. ISSN 0304-4238 DOI: 10.1016/S0304-4238(01)00236-9 Go to original source...
    22. PILAŘOVÁ, P., MARANDEL, G., DECROOCQ, V., SALAVA, J., KRŠKA, B. and ABBOTT, A. G.: Quantitative trait analysis of resistance to Plum pox virus in the apricot F1 progeny 'Harlayne' × 'Vestar'. Submitted
    23. Polák, J. and Pívalová, J., 2005: Sporadic distribution of Plum pox virus M strain in natural sources in the Czech Republic. Hort Sci, 32, 3: 85-88. ISSN 0862-867X Go to original source...
    24. Polák, J., Oukropec, I., Komínek, P., Krška, B. and Bitoová, M., 1997: Detection and Evaluation of resistance of apricots and peaches to plum pox virus. Journal of Plant Diseases and Protection, 104, 5: 466-473. ISSN 1861-3829
    25. POLÁK, J., OUKROPEC, I., KRŠKA, B., PÍVALOVÁ, J. and MILLER, W., 2003: Difference in reactions of apricot and peach cultivars to Plum pox virus: serological and symptomatological evaluation. Hort Sci, 30, 4: 129-134. ISSN 0862-867X Go to original source...
    26. Poncarová, Z. and Komínek, P., 1998: Restriction fragment length polymorphism differentiation of plum pox virus isolates. Acta Virol, 42, 4: 268-269. ISSN 1336-2305
    27. Rubio, M., Martínez-Gómez, P., Dicenta, F. and Audergon, J. M., 2004: Testing of genetic control hypothesis for Plum pox virus resistance in apricot. Acta Hort. 663: 265-267. ISSN 0567-7572 DOI: 10.17660/ActaHortic.2004.663.43 Go to original source...
    28. Salava, J., Polák, J. and Krška, B., 2005: Oligogenic inheritance of resistance to Plum pox virus in apricots. Czech J Genet Plant Breed, 41: 167-170. ISSN 1212-1975 DOI: 10.17221/3663-CJGPB Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY NC ND 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content