Acta Univ. Agric. Silvic. Mendelianae Brun. 2005, 53(4), 87-94 | DOI: 10.11118/actaun200553040087

Variabilita v genech pro leptin, leptinový receptor a protein vázající mastné kyseliny ve vztahu ke kvalitě masa u prasat

Renata Mikolášová, Tomáš Urban
Ústav morfologie, fyziologie a genetiky zvířat, Mendelova zemědělská a lesnická univerzita v Brně, Zemědělská 1, 613 00 Brno, Česká republika

Cílem práce bylo zjistit vztah tří kandidátních genů, respektive vybraných polymorfních míst genů leptinu (LEP-HinfI), receptoru leptinu (LEPR-HpaII), genu pro protein vázající mastné kyseliny (H-FABP-HinfI) a charakteristik důležitých pro kvalitu masa. V analýze byl použit soubor prasat plemen bílé ušlechtilé, landrace a durok pocházející ze stanice pro kontrolu výkrmnosti a jatečné hodnoty. Parametry kvality svaloviny, zjištěné na jatkách a v laboratoři Ústavu technologie potravin MZLU v Brně, byly: pH1, pH24, obsah myoglobinu, procento remise a obsah vnitrosvalového tuku. Z krve testovaných prasat byly genotypy stanoveny metodou PCR-RFLP. Frekvence alel jednotlivých polymorfismů byly stanoveny u celého souboru a také podle jednotlivých plemen. Dle χ2 testu byly genotypy ve sledovaných lokusech v genetické rovnováze. Vyhodnocována byla také kombinace genotypů LEPR x LEP, neboť se stále zkoumá vliv polymorfních míst těchto genů nejen na ukazatele tučnosti a je předpoklad také funkční závislosti u různých variant těchto genů. Výsledkem je potvrzení statisticky vysoce průkazného vlivu kombinace genů LEPR x LEP na hodnotu pH24 a statisticky průkazného vlivu na remisi. Průkazný vliv jednotlivých lokusů na vybrané ukazatele kvality svaloviny nebyl zjištěn, s výjimkou vlivu H-FABP-HinfI na vnitrosvalový tuk.

genetický marker, LEP, LEPR, H-FABP, prase, asociace, kvalita masa

Variability in the leptin, leptin receptor and heart fatty acid binding protein genes in relationship with meat quality traits in pigs

The leptin (LEP-HinfI), leptin receptor (LEPR-HpaII) and heart fatty acid binding protein (H-FABP-HinfI) genes and their genotypes combination (LEP-HinfI *LEPR-HpaII) were tested for associations with the pH1, pH24, myoglobin content (mg/100 g), intramuscular fat content (%) and remission (%). The genotypes were determined in Large White, Landrace and Duroc breeds (n = 106, 56 and 4, respectively). The allele frequencies were: LEP-HinfI: C = 0.133 T = 0.867; LEPR-HpaII: A = 0.331 B = 0.669; H-FABP-HinfI: H = 0.745 h = 0.255. The populations of breeds were in the genetic equilibrium according to the χ2 test in the tested loci. The combinations of LEP-HinfI and LEPR-HpaII were significantly associated with the pH24 and remission. The H-FABP-HinfI locus was significantly associated with intramuscular fat content.

Keywords: genetic marker, LEP, LEPR, H-FABP, pig, associations, meat quality
Grants and funding:

This study was supported by project MSM 432100001.

Received: January 25, 2005; Published: December 25, 2014  Show citation

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Mikolášová, R., & Urban, T. (2005). Variability in the leptin, leptin receptor and heart fatty acid binding protein genes in relationship with meat quality traits in pigs. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 53(4), 87-94. doi: 10.11118/actaun200553040087
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