Acta Univ. Agric. Silvic. Mendelianae Brun. 2005, 53(4), 61-74 | DOI: 10.11118/actaun200553040061

Vliv bakterií Staphylococcus aureus a Streptococcus uberis na morfologické projevy apoptózy neutrofilních granulocytů mléčné žlázy skotu in vitro

Tereza Langrová1, Zbyšek Sládek1, Dušan Ryšánek2
1 Ústav morfologie, fyziologie a genetiky zvířat, Mendelova zemědělská a lesnická univerzita v Brně, Zemědělská 1, 613 00 Brno, Česká republika
2 Výzkumný ústav veterinárního lékařství v Brně, Hudcova 70, 621 32 Brno, Česká republika

Cílem studie bylo posoudit vliv významných bakteriálních patogenů, Staphylococcus aureus a Streptococcus uberis na expresi programované buněčné smrti - apoptózy in vitro a stanovit dynamiku morfologických změn během procesu stárnutí neutrofilních granulocytů tkáňového poolu mléčné žlázy skotu in vitro. S použitím světelné a transmisní elektronové mikroskopie byly zaznamenány charakteristické změny apoptotických neutrofilů, které probíhaly ve třech na sebe navazujících stadiích. Bylo zjištěno, že interakce bakteriálních patogenů s neutrofily během inkubace vede k výrazným kvalitativním a kvantitativním změnám v zastoupení apoptotických buněk. Oba patogeny způsobují u neutrofilů mléčné žlázy opoždění projevů apoptózy. Výsledky potvrdily, že interakce S. aureus a S. uberis s neutrofily tkáňového poolu mléčné žlázy skotu in vitro způsobují změny týkající se apoptózy těchto buněk. Z výsledků práce lze vyvodit, že S. aureus a S. uberis jsou nejen významnými patogeny způsobujícími infekce mléčné žlázy, ale ovlivňují i buňky obranného systému mléčné žlázy a jejich funkci. Výrazně snižují počet buněk s morfologickými projevy apoptózy neutrofilů tkáňového poolu mléčné žlázy skotu. Počet apoptotických buněk v populaci neutrofilů potvrzuje, že interakce obou patogenů vede k oddálení nástupu morfologických projevů apoptózy. Vyšší počet apoptotických tělísek naznačuje rostoucí dynamiku tohoto procesu, která je rovněž ovlivněna specifikou bakterie.

programovaná buněčná smrt - apoptóza, polymorfonukleární leukocyty

The effect of the bacterial pathogens Staphylococcus aureus and Streptococcus uberis on morphological features of apoptosis of heifers mammary gland neutrophils

The aim of the student thesis was to review the effect of the significant bacterial pathogens, Staphylococcus aureus and Steptococcus uberis on programmed cell death - apoptosis in vitro, and to determine the dynamic of morphological changes during aging neutrophil granulocytes of heifers mammary gland in vitro. Using light and electron microscopy we have noted characteristic alterations of apoptotic neutrophils. These are running in three consequential phases. We found out, that the interaction of bacterial pathogens with neutrophils during the incubation have lead in expressive quantitative and qualitative changes in apoptotic cells proportion. Concretely, the influence of both pathogens on mammary gland neutrophils caused the defer of apoptosis expression. Here, S. aureus caused lower number of apoptotic neutrophils in comprasion with S. uberis. The outcomes testified, that S. aureus and S. uberis interaction with heifers mammary gland neutrophils in vitro causes alterations relating to apoptosis of these cells. Looking at the results of the study, we can conclude, that the pathogens S. aureus and S. uberis are not only significant heifers mammary gland - affection causers, but they significantly influence the cells of defensive system in their functions too. They significantly decrease the appereance of morphological apoptosis manifestations on neotrophils of tissue pool of the heifers mammary gland. The numbers of apoptotic cells in neutrophil population confirm, that during the interaction with mentioned pathogens the defer of morphological apoptosis manifestations happens. Then, higher number of apoptotic neutrophils in stages of apoptotic corpuscles implies increasing dynamic of this process. Beside that, the dynamic of apoptotic process is influenced by the specifity of certain bacterious actor too.

Keywords: programmed cell death - apoptosis, polymorfonuclear leukocyte

Received: February 17, 2005; Published: December 25, 2014  Show citation

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Langrová, T., Sládek, Z., & Ryšánek, D. (2005). The effect of the bacterial pathogens Staphylococcus aureus and Streptococcus uberis on morphological features of apoptosis of heifers mammary gland neutrophils. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 53(4), 61-74. doi: 10.11118/actaun200553040061
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