Acta Univ. Agric. Silvic. Mendelianae Brun. 2005, 53(2), 33-38 | DOI: 10.11118/actaun200553020033

Frekvence genotypů a alel PvuII polymorfismu genu estrogenového receptoru (ESR) u plemene bílé ušlechtilé a landrase

Eliška Goliášová1, Josef Dvořák2
1 Oddělení genetiky a biometriky, Výzkumný ústav živočišné výroby, Přátelství 815, 104 01 Praha Uhříněves, Česká republika
2 Ústav morfologie, fyziologie a genetiky zvířat, Mendelova zemědělská a lesnická univerzita v Brně, Zemědělská 1, 613 00 Brno, Česká republika

U populací hlavních mateřských plemen chovaných v České republice byly zjišťovány frekvence genotypů a alel PvuII restrikčního místa genu pro estrogenový receptor (ESR). Ze vzorků krve 1253 plemenic a 396 kanců plemene bílé ušlechtilé a 334 plemenic a 318 kanců plemene landrase byly určeny genotypy ESR modifikovanou metodikou PCR-RFLP podle Shorta et al. (1997). U kanců i prasnic plemene bílé ušlechtilé byla zjištěna frekvence alely B 0,51. U plemene landrase byla frekvence alely B 0,02 u kanců a 0,03 u prasnic. Genotypové frekvence u obou plemen nevykazovaly průkaznou odchylku od očekávaných frekvencí podle Hardy-Weinberga. Vývoj frekvencí alel poukazuje u plemene bílé ušlechtilé na zvyšující se frekvenci alely B, pravděpodobně způsobenou záměrnou selekcí zvířat genotypu BB. U plemene landrase byl pozorován opačný trend, který nemůže být na současné úrovni zjištění jednoznačně vysvětlen.

prase, velikost vrhu, kandidátní geny, ESR, frekvence

The oestrogen receptor gene (ESR) PvuII polymorphism allele frequencies in Czech Large White and Landrace

Genotype and allele frequencies of the oestrogen receptor gene (ESR) PvuII restriction site were investigated in populations of the main Czech maternal breeds. 1253 sows and gilts and 396 boars in Large White and 334 sows and gilts and 318 boars in Landrace were genotyped from blood samples by the modified PCR-RFLP procedure as described in Short et al. (1997). In Large White, the frequency of allele B was about 0.51. In Landrace, the frequency of allele B reached from 0.02 for boars to 0.03 for sows. No significant deviations of the observed genotype frequencies from the frequencies expected according to Hardy-Weinberg equilibrium were found in both breed. Opposite trends in allele frequencies development could be assumed for analysed sows and boars of both breeds. In Large White sows the frequency of allele B raised probably due prefering sows with BB genotype, whereas in Landrace population the frequency of allele B decreased from yet unknown reasons.

Keywords: pig, litter size, candidate gene, ESR, allele frequencies
Grants and funding:

The research was supported by the project QD 1039 of the National Agency of Agricultural Research (NAZV) of the Czech Republic.

Received: January 28, 2004; Published: December 28, 2014  Show citation

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Goliášová, E., & Dvořák, J. (2005). The oestrogen receptor gene (ESR) PvuII polymorphism allele frequencies in Czech Large White and Landrace. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 53(2), 33-38. doi: 10.11118/actaun200553020033
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