Acta Univ. Agric. Silvic. Mendelianae Brun. 2014, 62, 1041-1048
Published online 2014-12-02

Microbiological Quality of Experimental Silages in Combination With the Addition of Topsoil Soil Layer and Ensiling Additives

Veronika Mlejnková1, Martina Fröhdeová1, Libor Kalhotka2, Petr Doležal1

1Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic
2Department of Agrochemistry, Soil Science, Microbiology and Plant Nutrition, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic

Fodder crops contamination by the surface layer of soil is important in terms of the occurrence of clostridial spores that can infect silage through the contaminated fodder crops, which is followed by the feeding and occurrence of clostridia in the environment of the stalls resulting in their presence in milk, milk products and finally in the human digestive system. The main objective of the submitted study was to assess the impact of added topsoil layer and ensiling additives on the hygienic quality of the experimental silages. In the model experiment, we used alfalfa from the second cut. In total, the experiment included 9 various treatments in three repetitions. Use was made of the variants of experimental silages P0, P20, P40 (without ensiling additive), B0, B20, B40 (treated with a biological ensiling additive) and CH0, CH20, CH40 (treated with a chemical ensiling additive) always with an addition of a surface layer of soil in a quantity of 0, 20 and 40 g/kg of dry matter. The model silages were assessed after 10-week storage. A representative sample was taken from each experimental variant to perform a microbiological analysis. In the experiment, the counts of clostridia colonies, sporulating microorganisms, anaerobic microorganisms, total number of microorganisms (CPM), lactic acid bacteria, moulds and yeasts were observed. Statistically conclusive difference in the topsoil layer addition was only proved in CPM between variants P20 (1.96E+07 ± 6.21E+06) and P40 (4.97E+07 ± 1.89E+07) and bacteria of the Enterobacteriaceae family between variants P0 (2.17E+02 ± 3.32E+01) and P40 (3.64E+01 ± 2.57E+01). A positive effect of ensiling additives on the microorganism growth inhibition was determined.


The study was supported by the Research Plan and internal grant project (IGA) No. IP 2/2012 “The risk incidence of clostridia in silage.”


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