Acta Univ. Agric. Silvic. Mendelianae Brun. 2011, 59, 153-160
Published online 2014-03-16

In vitro testing of defense reactions in zygotic and somatic embryos of Abies numidica

Jiří Hřib1, Václav Adamec2, Božena Vooková3

1Ukrajinská 17, 625 00 Brno, Česká republika
2Ústav statistiky a operačního výzkumu, Mendelova univerzita v Brně, Zemědělská 1, 613 00 Brno, Česká republika
3Ústav genetiky a biotechnológií rastlín, Akademie vied SR, Akademická 2, P.O. BOX 39A, 950 07 Nitra, Slovenská republika

Defense of desiccated cotyledonary somatic embryos and mature zygotic embryos of Abies numidica was tested in vitro by dual cultures with tester, fungus Phaeolus schweinitzii. Both types of embryos expressed defense reactions manifested by inhibited growth of fungal tester towards the embryos. Mycelial growth was described by logistic sigmoid growth model with a single asymptote. Mutual comparisons of mycelial growth in presence of zygotic and somatic embryos showed significant differences in parameters of mycelium growth curves towards the embryos. Larger defense reactions were observed in zygotic embryos relative to somatic embryos and unlimited control cultivations without embryo. The possible role of auxin in the defense response of plant embryos is discussed.


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