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The effect of known CSN3, Pit-1 and LGB genes single nucleotide polymorphism (SNP) on milk performance traits (milk yield, protein, fat and lactose content, count of somatic cells) at the Czech Fleckvieh and the Holstein cattle breed was estimated. For more exact results, tested cows of each breed came from one source, and were selected into two groups according to their lactation. We tested around 100 animals at the first and 25 at the high (more than five) lactation of both breeds. Our attention was aimed particularly on combined genotypes of favourable alleles. We confirmed the positive tendention of the cheese yield valuable CSN3/BB with Pit-1/AA combined genotype for high milk production. By estimation of single gene effect, we found out significant association (P ≤ 0.01) between milk yield (kg) and Pit-1 genotypes at Holstein cows with the high lactation. This dependence was observed at the Czech Fleckvieh breed too, even it didn't get conclusive value. Among all tested individuals, CSN3 genotypes significantly influenced milk yield (AA > BB, P ≤ 0.01), milk fat (BB > AA, P ≤ 0.01) and protein content (BB > AA, P ≤ 0.01). The genotypes AB and BB of the LGB gene at Holstein cows were joined with higher milk yield in selected cattle groups opposite to AA genotype (P ≤ 0.01, P ≤ 0.05). The influence of LGB on the somatic cell count in created cattle groups was not confirmed. Estimation of allele and genotype frequency according to lactation stage didn't reveal higher differences at non of tested genes in both breeds.

Global shape of the peaches of the Red Haven variety (harvested on different days during July, 2007) has been characterized by calculating the H/Ds, H/Dc and Dc/Ds ratios. There is no, or nearly no, differences between peaches harvested at the different days. The shape of the all tested peaches was very close to round one. The more detailed description of the peach shape is based on the Elliptic Fourier analysis (EFDs) of the digital photos. The knowledge of the parameters (curvature etc.) is very useful e.g. for the evaluation of the results of the strength tests. Compression tests have been also perforemd - the whole peaches have been compressed between two plates at the constant cross-head velocity 20 mm/min. The force-displacement curves are characterised by certain monotonic increase and the point called bioyield, where force exhibits a drop. The bioyield significantly decreases with the date of the harvesting. There is no evidence of the dependence of this parameter on the direction of loading. The same result is approximatively valid for apparent modulus of elasticity E. The force-displacement curves have been successfully modelled by non-linear mathematical models.

Identification of improvement areas and utilization of information and communication technologies have gained value and priority in our knowledge driven society. Rules define constraints, conditions and policies of how the business processes are to be performed but they also affect the behavior of the resource and facilitate strategic business goals achieving. They control the business and represent business knowledge. The research works about business rules show how to specify and classify business rules from the business perspective and to establish an approach to managing them that will enable faster change in business processes and other business concepts in all areas of the business. In concrete this paper deals with four approaches to business rules formalization, i. e. notation of OCL, inference rules, decision table and predicate logic and with their general evaluation. The article shows also the advantages and disadvantages of these approaches of formalization. They are the example of every mentioned approach.

Resistance to lime-induced chlorosis is very important characteristic of grapevine rootstocks. Lime-induced chlorosis influence yield and quality of grapes. Evaluation of chlorosis resistance is very important for grape growing and breeding. The aim of this work is provide results of evaluation grapevine rootstocks to chlorosis. High resistance was show in Fercal. From the viewpoint of the resistance to chlorosis, the rootstocks registered in the State Variety Book of the Czech Republic can be ranked from the most resistant to the most sensitive as follows: Craciunel 2 - SO 4 - Kober 125 AA - Kober 5 BB - Teleki 5 C - Amos - LE-K-1.

The effect of N and P fertilization on the dry matter content, concentration of nutrients (N, P, K, Ca and Mg) and their intake by the plant, production characteristics (achene yields, head diameter, 1000-seed weight, oil content and oil production) and the content of fatty acids (palmitic, stearic, palmitooleic, oleic, linoleic and linolenic) was evaluated in the one-year pot trial with sunflower (Helianthus annuus, L.). Nitrogen application had a positive effect on dry matter production and increased N intake by plants as early as the beginning of vegetation (4^th true leaf). The effect of P on dry matter production was not markedly evident until the later growth stage (10^th true leaf). At the end of the vegetation period the weight of plants of variants fertilised with N only and variants where P was applied evened out. Achene yields, head diameters, 1000-seed weight, oil content and oil production increased statistically significantly after N application. Of all the monitored characteristics the increased level of P in the soil affected only the 1000-seed weight. The application of both nutrients did not significantly change the content of fatty acids.

Series of mechanical tests (uniaxial compression test, Brasil test, plate bending test) with cylidrical specimens made from dark chocolate have been performed. The stress-strain dependence has been ploted and modeled using data from compression test. The strain behaviour of the chocolate was found to be far from the linear elastic strain. The linear elasticity is limited for the very small strains. The Brasil test led to the development of the tensile stress in the specimen and its cracking. The dependence of the tensile stress on the strain rate has been confirmed. Also the linearity of the force vs. displacement data received during plate bending test has been limited to the very small value of the displacemnt. Impact behaviour of the chocolate has been also evaluated. Falling of the specimen from defined hight led to its fracture. This experiment has been simulated using LS DYNA 3D finite element code.

One of the most important parts in a precision farming system is the crop yield mapping. The pre-processing of the point data from combine harvesters, the transfer of the yield data onto the management grid and the interpolation of the yields are discussed, as well as using of the yield data themselves. The goal of the contribution was to verify, with the help of the field measuring on two stations, the dependence of the yield obtained by processing of the measured yield data from the combine harvester (where the yield monitor is installed) on the weighted yield. This is because of yield variability, which is the basic dependent variable of majority belonging to the rest kinds of field variation that provides the basic information about the field. Afterwards it is possible to transfer the yield data as the base for the variable application of the fertilizer. By this we access yield effects for the different site - specific treatment.

This paper is focused on validation of new synthetic raw cow milk quality indicator which was calculated according to previously developed algorithm. The aim was to carry out a synthesis of values of individual milk quality indicators (MQIs) into one standardized relative milk quality indicator (SQSM). This is useable for farmer milk price modification according to milk quality in dependence on its change in every case. It is a possible ground for consistent milk payment according to its quality. This work is fifth step in the row of works. It is carried out in terms of next promotion of the milk foodstuff chain safety and for the improvement of the ability of the dairy production branch for the competition. SQSM was calculated according to formula as follows: DX = (IND - x)/sx, where: IND is individual value of MQI of supplier, x is month arithmetical average of MQI of relevant milk suppliers including IND and sx its standard deviation; SQSM is the sum of logically oriented (according to milk quality growth) DXs, which come from relevant MQIs of identical raw milk delivery; SQSM as sum can be also divided by the number of MQIs. It means, there are possible two kinds of expressions of SQSM, sum (a) and/or average (p). The values of MQIs were used in original (compositional and physical MQIs) or transformed (log; hygienic MQIs) form according to previous research results. Three, five and seven MQIs and single, double and threefold values of their technological weight were included into model validation as price formula. Thirteen variants (p and a) of price formula were used, too. The behaviour of p and a model variants was similar. All SQSM averages and medians were very near zero value. The normality of SQSM files was reached (P > 0.05) sometimes in obliquenes (a₃) which is more important in this case. The normality was not reached in acuteness (a₄). The SQSM model files were quite well balanced in terms of positive and negative values. All model combination differences (p) between variants were insignificant (calculated F 1.76×10^-18, where P > 0.999; critical F 1.75 for P ≤ 0.05). The difference insignificance was independent both on number of MQIs and on their technological preference as well. The results of SQSM validation showed the suitability of indicator usage for intended purpose of consistent milk price modification according to every milk quality change.

Intensity of snowmelt erosion was computed for cadastral areas of Pohořelice, Strážnice, Kuchařovice, Holešov, Dukovany, Vizovice, Velké Meziříčí and Bystřice nad Pernštejnem on the base of 1980-2006 data for the period November to March. Mean long-term soil loss was estimated according to ZACHAR (1981) equation on the base of average rate of snowmelting, amount of melting water, outflow and infiltration characteristics, topographical factor, soil factor and vegetation factor. For the studied localities the average rate of snowmelting varied from 2.29 mm.day^-1 to 7.90 mm.day^-1. Also amount of melting water reached the maximum in Bystřice nad Pernštejnem in dependence on climatic region (6.11 cm of water column). Outflow and infiltration characteristics varied from 0.94 to 1.14. High value of topographical factor (10.5) was assessed in Vizovice. Soil factor was estimated according to main soil unit from Estimated Pedologic-Ecological Unit EPEU. Its values varied from 0.31 in Velké Meziříčí to 0.47 in Holešov. Vegetation factor varied from 0.2938 in Vizovice to 0.4881 in Kuchařovice. Average soil loss as a consequence of snow melting varied in interval from 0.61 t.ha^-1.year^-1 in Pohořelice to 30.08 t.ha^-1.year^-1 in Vizovice.

The objective of the present study is to characterize the rheological properties of peaches according to the results of puncture test force/displacement curves. The measurements were carried out on the universal testing machine. The firmness of the peaches was measured on the equatorial side of the fruit. Four measurements were carried out on each fruit by varying the orientation at intervals by about 90°. The used approach computed six texture parameters from the curves, which were supposed to be representative of skin hardness, fruit deformation before skin rupture, flesh firmness and mechanical work needed to penetrate the fruit. The data were fitted by an exponential function. The correlation is not statistically significant. Its value only support the hypothesis about decrease of the skin strength and other values. Generally, there is a decreases with time from the harvest beginning but the scatter of the data is too high. The results show that used method enables to describe the peach firmness development during the harvest period but the effective use of this method is based on the large number of experiments which are destructive in their nature. Some new approach is briefly outlined.

The paper deals with problems concerning customers' satisfaction. It summarizes results of primary research focused on an analysis of fundamental factors, which shape clients' preferences, influence the level of satisfaction of transportation companies' customers in general and in transportation companies under study in particular. Altogether 49 % of respondents mentioned that the type of transportation means was the most important factor that influenced their satisfaction. As other significant factors influencing the satisfaction of respondents the following were mentioned: prices of transportation, quality of services during the transportation, respecting regular pauses during transportation, and the possibility of refreshments during the transportation. All companies that were addressed monitored the level of satisfaction of their customers (nearly 20 % did this every month). Each company that monitored satisfaction of its customers every year also developed a marketing plan of their activities. The paper also contains detailed results of conducted inquiries as well as the relevant discussion.

A two year-experiment was carried out to study the effect of root inoculation with two biopreparations, Vambac^® (VA-mycorrhiza genus Glomus, Gigaspora and the rhizospheric bacteria Agrobacterium radiobacter) and Amalgerol^® (composed of vegetative and sea-algae oils and extracts) on leaf mineral concentration, vegetative and generative behaviour in two year old apple trees cv. 'Jonagold'/M.9. Trees were grown in 10 l plastic pots in unsterilized sandy clay soil (Fluvisols) under climatic conditions of South Moravia (49°,25' N; 16°,55' E), Czech Republic. Leaves were sampled eight times during the experiment (four times per year) for analysis of: N, P, K, Ca, Mg, Zn, Fe, Cu, and Mn. Mycorrhizal infection was determined with the gridline-intersection method. Inoculated plants showed higher VA root infection during both years (best results were 46% on the first year and 41.7% on the second year) and higher leaf P concentrations, in particular when biopreparations were applied together (P = 0.23% DW), than non inoculated (4% of root infection during year one and 15% during year two; P = 0.183% DW). N, K, Ca and Mg were not significantly affected by treatments. In many cases application of biopreparations decreased Fe, Mn and Zn leaf levels. Shoot length was strongly enhanced in inoculated plants only during the first year (46% more than non inoculated). No significant differences were detected between inoculated and non inoculated plants for: trunk diameter, spur number, root volume, leaf dry weight, number of blossoms and yield. It is concluded that the studied biopreparations have the capacity to colonize and persist in the roots of apple trees (VA-mycorrhizal fungus), enhance the uptake of phosphorus and vegetative growth but they could decreased the uptake of Fe, Mn and Zn.

Demographic evolution in the Czech Republic is not spatially homogeneous but displays regional differences. In the set of 14 regions of the Czech Republic, numerical characteristics showing level, variability, skewness and kurtosis for 30 demographic indicators from the year 2006 are calculated and evaluated. Frequency counts for some of these have been shown using histograms. On the basis of factor analysis, five representative indicators were chosen and assigned weights, with the ranks of regions determined by normalized values. The best position was obtained by the City of Prague and Středočeský Region, with last position occupied by the Zlín Region.

In a small-plot trial with potatoes were applied increasing doses of selenium to the soil (0, 12, 24, 48 and 72 kg Se.ha^-1) and after harvest were explored the content of selenium, phosphorus and sulphur in raw tubers and in the tops. The trials were conducted in two localities - Žabčice and Valečov using the semi-early variety Ditta. Selenium was applied to the soil in the form of sodium selenite before planting the potatoes. Chemical analyses discovered that with the applied dose the level of selenium increased in both tubers and tops and that the correlation between the applied selenium dose and its content in tubers and tops was significant (α < 0.05) and positive (r = 0.885 and r = 0.908, respectively). With an increasing dose of selenium the reduction in the level of sulphur in the tops (r = -0.872, α < 0.05) was statistically significant. In both localities the range of the sulphur levels in tubers was very narrow, i.e. 0.16-0.18%, and the effect of the selenium dose was not significant. The phosphorus content in tubers and tops did not change significantly after application of a dose of up to 48 kg Se.ha^-1. With the highest dose of selenium the content of phosphorus in both tops and tubers decreased significantly.

The goal of this paper is to investigate the effect of the end distance of cam lock fasteners on the bending moment resistance of knock-down corner joints. The preliminary study of knock-down furniture assembly plans was done in order to discover the manufacturers' fastener typical usage in case construction. Laminated particleboard, cam fasteners and wooden dowels were used for specimen construction. L-shaped joint specimens 760 mm in length were tested by pressing the joint members together - also called a compression test in the angle plane. The study results showed that cam fasteners with end distance of 60 mm from the member edges perform the best.

During the vegetative period of the year 2005 (June-October) we carried out hydrobiological and hydrochemical monitoring of selected ponds inhered in Southern Moravia in relation to different fishery management intensities. Water temperature, dissolved oxygen content, pH, conductivity and water transparency were monitored directly at taking place, N-NH₄⁺, N-NO₂^-, N-NO₃^-, P-PO₄^3- content and chlorophyll-a concentration were measured in hydrochemical laboratory. At the same time, water samples for taxonomical analyses of phytoplankton and assessment of toxin content of cyanobacteria were taken. All ponds were characterized by low water transparency, high values of pH, N-NH₄⁺, P-PO₄^3- and high chlorophyll a concentration. We detected maximum concentration of 18.7 µg.l^-1 microcystins in ponds dominated by the species Microcystis aeruginosa. Total microcystin concentration in water of all ponds with dominance of pikoplanktonic cyanobacteria were below detection limit (LOD < 0.125 µg.l^-1). The dominance of cyanobacteria species shifts from coccal (especially genus Microcystis) to small-colonial pikoplanctonic (genus Aphanocapsa, Aphanothece) and to single-filamentous (genus Planktothrix, Limnothrix, Pseudanabaena) cyanobacteria related to increasing fish stock density.

Over the period of July to September 2005 effects of insecticide Integro with the effective substance methoxifenozide and biological preparation Trichoplus using parasitoid wasps Trichogramma evanescens and T. pintoi were evaluated in maize stands. These natural enemies of aphids were recorded in monitored fields (located in South Moravia, Czech Republic): parasitoids of genera Aphidius and Praon (Hymenopetra, Aphidiidae), predatory syrphid flies (Diptera, Syrphidae), ladybirds (Coleoptera, Coccinellidae), Orius bugs (Heteroptera, Anthocoridae), Green Lacewing - Chrysoperla carnea (Neuroptera, Chrysopidae) and spiders (Araneida). No significant difference (T = 0.003; F = 4.894; P = 0.004) in populations of aphids and their antagonists was found in the studied variants (Integro, Trichoplus). More remarkable differences were found only soon after spraying with Integro. Syrphid flies pupae decreased by 41% in the Integro variant, and by 13% in the Trichoplus variant. Ladybird larvae did not occur in the Integro variant after spraying, there where 0.12 ladybird larvae on plant in the Trichoplus variant. Number of ladybird adults increased from 0.01 to 0.53 individuals per plant in the Trichoplus variant, but from 0.03 to 0.1 individuals in the Integro variant. Number of ladybird pupae decreased from 0.32 to 0.02 pupae per plant in the Integro variant, but it has increased from 0.04 to 0.54 in the Trichoplus variant. Correlative relationship between ladybird adult growing coefficient and aphid populations in the Trichoplus variant was found.

Bog pine (Pinus uncinata DC. subsp. uliginosa (Neumann) Businský) is a subendemic species and appears to be one of the most endangered tree species of the Czech Republic. Its rare populations are at the present time greatly endangered namely by spontaneous hybridization with the Scots pine (Pinus sylvestris L.). Regarding the fact that its protection is insufficient even in national nature reserves (NNR) and the classical propagation by cuttings is problematic, modern methods were adopted for a long-term preservation of the taxon. Growth regulation conditions were investigated for the induction of organogenesis and somatic embryogenesis. Mature seeds were obtained from selected trees in the Žofinka NNR and from a locality near the village of Krajková in the Sokolov district. Cotyledon and hypocotyl segments from seedlings pregerminated in sterile conditions were used to induce the organogenesis. Bud proliferation was observed on the cotyledons after 4 weeks. The largest amount of buds was found on the medium with 1.5 mg.l^-1 benzyladenine (BA) and 0.5 mg.l^-1 1-naphtylacetic acid (NAA) after 12 weeks. The hypocotyl segments showed only the formation of green callus. Isolated mature zygotic embryos were used for the induction of somatic embryogenesis. Development of mucilaginous callus was recorded after 3 weeks of cultivation on media with different combinations of BA, NAA and 2,4-dichlorophenoxy acetic acid (2,4-D) concentrations. When the callus induced on the medium with the combination of 0.56 (1.13) mg.l^-1 BA and 1.86 mg.l^-1 NAA was transferred on the medium with lower BA (0.113 mg.l^-1) and 2,4-D (0.44 mg.l^-1) concentrations, the first somatic embryos started to emerge after a period of other two weeks.

In the April of 2007, pseudomyiasis was detected in alimentary tract of two years old boy from South Moravia (Czech Republic). Contamination was caused by larvae of family Calliphoridae (Diptera), accidentally consummated with half-rotten fruits from bio compost. It is the first recent documented case of pseudomyiasis, and is closely connected with bio waste and ecological waste disposal. Usage of organic waste composting is increasing all around the world, motivated by demand for maximum recycling. While workrooms of bigger compost facilities are usually separated from public, waste containers in front of the houses or small composts in the gardens serving one family are freely accessible to public. These bio composts and waste containers attract many species of insects, especially from order Diptera. so for inhabitants of neighbouring houses this can represent certain health risk. In the location of findings, Dipteras were caught and their species spectrum was evaluated considering their ability to cause myiases or pseudomyiases.

Publicly traded companies prepare their consolidated accounts in conformity with the international accounting standards (IAS/IFRS) in accordance with the Regulation No. 1606/2002. This is obliged for all publicly traded joint-stock companies in the Czech Republic. Other companies prepare financial statements in accordance with national accounting standards. There are Accounting Act No. 563/1991 of Coll. and Regulation No. 500/2002 of Coll., Czech Accounting Standards in the Czech Republic. Both systems are based on different principles so there are many differences. The Czech Accounting System (CAS) is based on the rules while IAS/IFRS are based on principles (Kovanicová, 2005). These differences are mainly caused by the different philosophy. CAS prefers the fiscal policy to the economic substance while IAS/IFRS prefere the economic substance. One of the most significant differences is in the field of revenue recording. There are two standards concerning the revenues recording (IAS 18 - Revenue, IAS 11 - Construction Contracts) in IAS/IFRS. CAS 019 - Expenses and Revenue are dealing with the revenue recording in the Czech Republic.
The paper is aimed at the comparison of the methodical approaches for revenue recording used by IAS/IFRS and by CAS. The most important differences are caused by the different approach to the long term contracts (construction contracts, software development contracts) revenues recording.

In this work were summarized changes of biochemical markers of fish under the thumb of cyanobacterial toxins (microcystins). Among the most studied biomarkers of the influence of cyanobacterial toxins on fish belong oxidative stress parameters - glutathione S-transferase (GST), non-enzymatic antioxidant glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), lipid peroxidation (LPO), malondialdehyde (MDA), glutatione reductase (GR), parameters of blood - values of haemoglobin (Hb), haematocrit (PCV), mean corpuscular haemoglobin concentration (MCHC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), erythrocyte (RBC), leukocyte counts (WBC) and plasma - alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), cholinesterase (CHE), total serum protein (TP), glucose (GLU), lactate (LACT), iron (Fe), calcium (Ca), magnesium (Mg), total bilirubin (BIL), phosphorus (P) and protein phosphatase activities (PP1, PP2A).

Complete faunistics was compiled in countries surrounding the Czech Republic and Slovakia. The latest checklist of the bee fauna from the then Czechoslovakia being published well over a decade ago, the aim of this paper is to up-date Kocourek's checklist (hereinafter referred to as only "the List") from 1989.
In the present paper, inaccuracies occurring in Kocourek's checklist (e.g. using of junior synonyms or homonyms, spelling of names, incorrect species distribution, etc.) are corrected. In addition, new records according to literature data, findings communicated by colleagues, or own records, are summarised.
The compilation of this faunistic list required checking of over 750 names of the species group and more than 140 supraspecific names to be used valid and available names. The faunistic revision results in the following findings.
In total, 431 bee species range the Bohemian territory (occurrence was approved); of that, the following species are newly included: 4 species according to records (captured or checked specimen(s)) and 3 species as per literature data. Five species are conditionally removed from the Bohemian fauna, ten species are missing, eleven species are removed from the checklist, and the occurrence of one species is potential.
Altogether 543 species range the Moravian territory; in that number, the following species are newly included: 17 species according to records, and 6 species according to literature data. Eight species are conditionally removed from the Moravian fauna, one species is missing, six species are removed from the checklist, and the occurrence of five species is potential.
A total of 625 species range the Slovak territory; of that, the following species are newly included: 17 species according to records, and 14 species according to literature data. Seventeen species are conditionally removed from the Slovak fauna, no species is missing, ten species are removed from the checklist, and the occurrence of thirteen species is potential. Therefore, more intensive faunistic research is recommended in the Slovak territory.
Altogether 655 species range the Czech Republic and Slovakia; of that number, 555 species occur in the Czech Republic, and 625 species occur in Slovakia. It results in finding that the diversity of the bee fauna in the Czech Republic and Slovakia can be regarded as comparablely equal with their neighbouring countries regarding the structure and geographic position of the both republics.
The most important records of species are as follows: Hylaeus trinotatus (Pérez, 1895) [Slovakia], Andrena pontica Warncke, 1972 [Moravia], Halictus resurgens Nurse, 1903 [S], H. smaragdulus Vachal, 1895 [M, S], Lasioglossum bavaricum (Blüthgen, 1930) and L. cupromicans (Pérez, 1903) [S], L. lissonotum (Noskiewicz, 1926) [M, S], Sphecodes majalis Pérez, 1903 and Melitta wankowiczi (Radoszkowski, 1891) [S], Megachile flabellipes Pérez, 1895 [M], Coelioxys alata Förster, 1853 [S], Anthidium septemspinosum Lepeletier, 1841 and Melecta aegyptiaca Radoszkowski, 1876 [M], Pyrobombus (Cullumanobombus) semenoviellus (Skorikov, 1910) [Bohemia] and P. (Melanobom

R. solani (Thanatephorus cucumeris (Frank) Donk) isolated from the sclerotia from the potatoes belongs to anastomose group 3 (AG 3), defined as the "potato type". The present study shows the results of research into the efficiency of the seed-fungicides on R. solani (AG 3) isolates from various regions (12) of the Czech Republic. In addition to registered seed-fungicides we also tested the unregistered preparation Prestige 290 FS (pencycuron + imidacloprid). Our objective was to test the efficiency of the seed-fungicides on R. solani isolates and the sensitivity, or resistance, of the isolates to registered and unregistered seed-fungicides and/or to the active ingredients mancozeb, tolclofos-methyl, pencycuron and carboxin + thiram, and to the unregistered combination of the pencycuron + imidacloprid active ingeriens.

Due to their worldwide distribution and also detrimental potential virus diseases of grapevine are economically very important. In grapevine plants Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV) is one of the most harmful virus pathogens. The aim of this study was evaluate the occurrence of GFLV and ArMV in vineyards of the Grapevine Breeding Station Polešovice (Czech Republic) using the ELISA test and visual symptoms. In every year from 2001-2004 altogether 225 grapevine plants were tested for the occurrence of GFLV and ArMV. In case of GFLV positive results were obtained in 43 plants, i. e. in 19.11% of all samples. In case of ArMV there were 51 positive tests, i.e. 22.66%. Our results demonstrated a occurrence of GFLV and ArMV in propagation vineyards, which was comparable with results obtained abroad. It was also demonstrated that a professional visual selection could provide a good basis for the identification of virus diseases and their subsequent tests.

Conformity Assessment of construction products is in accordance with EU concepts enabling free movement of goods within member states. The construction products (i.e. also construction product based on wood) which are liable to conformity assessment are listed in the Governmental Decree No. 312/2005 Coll. and Governmental Decree No. 190/2002 Coll. (technical requirements for construction products with CE marking). The European harmonised standards (both issued and prepared) have immediate influence on methodologies of Conformity Assessment. Methodologies and requirements on products during this process implicate also requirements on Notified Bodies and producers. The aim of the first stage of the research was to analyse the present state in this field and relations taking place during preparation and implementations of harmonised standards.

In 2004 and 2005 we explored the effect of graded doses of selenium in the soil (0, 12, 24, 48 and 72 kg Se.ha^-1) on yield-forming parameters (total plant weight, number of stems per hill, number of tubers per hill and hectare yields) in two varieties of potatoes of different vegetation periods. The content of selenium as an important anti-oxidant was monitored in potato tubers and tops. Selenium in the form of sodium selenite was applied in the respective doses into the soil before planting the potatoes. Samples for growth and chemical analyses were taken after 90 and 99 days of vegetation (variety Karin and Ditta, respectively). Increasing doses of selenium had a negative effect on most of the yield-forming parameters. With an increasing dose of Se the hectare yields as well as the number of tubers in the hill and total weight of the plant decreased. Chemical analyses were performed using the AAS method and showed that the concentration of selenium in the individual parts of the potato plant increased with increasing Se doses in the soil. The content of selenium in tubers of the variant Se₇₂ (4.13 mg Se.kg^-1 of dry matter) increased as much as 20 times when compared to the control (0.22 mg Se.kg^-1 of dry matter).

The results of the previous studies have suggested that the estimated R_G values are loaded with an error, which might compromise the precision of the subsequent crop model applications. Therefore a detailed analysis of the error propagation was made using two crop models i.e. CERES-Barley and CERES-Wheat. Database of meteorological data originating from 8 stations in Austria and Czech Republic was used in order to carry out the analysis. It has been found that even application of the method based on sunshine duration that yield the lowest bias in R_G estimates significantly influences number of key crop model outputs. It has been also noted that in 5-6 seasons out of 100 cases the deviation greater than ±10 % is to be expected whilst the occurrence of ±25% could not be also ruled out. The precision of the yield estimates and other crop model outputs is lower then expected but still acceptable for most application with mean bias error in range of 2.0-4.1% when estimates based on the diurnal temperature range and cloud cover are used. In this case yield deviations over ±10% occurred in about 20% cases (depending on the crop) whilst the probability of significant yield departure (±25%) doubled of that found for the previous method. The methods based on the diurnal temperature range and daily precipitation sum showed an increase of the systematic bias of yield of winter wheat and considerably higher number of seasons with yield departures over ±25%. Utilisation of the methods based on the diurnal temperature range only for the purposes of seasonal yield forecasting or climate change impact assessment is questionable as the probability of significant yield departure is very high (as well as the systematic error). These findings should act as an incentive to the further research aimed at development of more precise and widely applicable methods of estimating daily R_G based more on the underlying physical principles and/or remote sensing approach. Overall decrease of the existing uncertainties in the R_G estimates would clearly result into increase of the reliability of subsequent applications that use R_G as input variable.

The main goal of this paper was to create theoretical basis and find starting points for the audit of customer satisfaction on the service market. It focuses mainly on contemporary existing approaches to customer satisfaction quantification especially through indexes. The gained results will become the starting point for creating the consistent audit of customer satisfaction methodology on tourism service market applicable in the Czech environment. One part of the paper shows brief results of the performed tentative research that aimed for finding present awareness about the audit of customer satisfaction and about the frequency of its use by firms in the Czech Republic.

Commercially produced hen eggs have been tested by means of dynamic excitation of the egg-shells with following analysis of their response. The falling steel ball have been chosen as a exciting instrument and the laser vibrometer have been used as a measuring device for the egg response. The reproductibility of the experiments has been relatively high and the surface velocity has been found to be significantly dependent on the position around the meridian. Analysed frequency spectrum has shown the peak frequency and frequency history. Proposed numerical model has demonstrated reasonable agreement with experimental results and can be used as an effective tool in modelling of analogous or similar experiments.

This research work is aimed to monitoring of selected disinfectants'activity in operational conditions. Hereby there have been monitored two acidic disinfectants Despon K and Mikasan D, which have had-by their producer-stated different recommended concentration. These solutions were monitored in viewpoint of their activity at different temperature, time of circulation, pH and water hardness. In this work there were measured pH of solutions in unloaded medium to be compared with pH of solutions in loaded medium and this measuring was carried out regularly each week within a one month period. During this period there was also monitored total plate count (TPC), which was stated in the dairy, where samples were taken two-times monthly. It has been found, that the disinfectants Mikasan D and Mikal 94D are effective even by high water hardness.