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The objective of this study was to determine the effect of herd, breed, the age of rams, their semen density, and the type of diluter used on sperm motility during a survival test. The total level of sperm activity of 49 rams was evaluated. A statistically significant effect of the herd, breed, the age of the rams, and the density of their semen on sperm motility after 30, 60, and 90 minutes was determined. Significant differences among herds were detected in the level of sperm activity after 90 minutes of the survival test only, P < 0.05. A significant difference (P < 0.05) between breeds was determined only in the case of sperm activity after 60 minutes of the test. Higher sperm motility during the entire survival test was detected in Bohemia Forest rams. A non-significant difference (P > 0.05) was found in the sperm activity in relation to the age of the rams. Sperm survival during the test significantly differed in relation to sperm density before diluting (P < 0.05). No significant differences among diluters used were confirmed. Relationships among sperm activity before and after diluting and during the entire survival test were confirmed by significant Pearson's correlation coefficients (P < 0.001).

In 2007, when the pilot project of Home State Taxation System should started, but none of the EU Member States applied for, the European Commission has turned its attention to different project in the area of corporate income taxation. The paper presents the problems of consolidation under the system of Common Consolidated Corporate Tax Base, which is at present the aim of the European Commission in the area of corporate tax harmonization. Firstly, the paper presents the results of comparative analysis, which have been done throughout the EU Member States. The research was aimed at the area of group taxation schemes availability. Secondly, the paper presents the draft of CCCTB directive in the field of creation of the group for taxation purposes, the rules for access and exit from the group and the rules for calculation of thresholds for voting rights. The different possibilities of group creation are presented on the schemes. The paper also discuss the rules, suggested by the draft directive, which could create legal uncertainty for the companies and could cause the situation in which the companies would not know whether they can consolidate their accounting results or not, or whether they are the member of the group or not. The paper suggests the possible solutions in that area. At the end, there are also mentioned and discussed the methods, which could be used for consolidation under CCCTB system in the EU.

In the present study was estimate the genetic diversity and relationships among nine horses breeds in Czech and Slovak Republic.
In conclusion, the main objective of study was to show the level of genetic distance among the horse breeds with different history of breeding of each country. Furthermore, it should be clarified whether these populations and subpopulations are distinct enough from each other to justify defining separate breeds. This research concerns the variability of microsatellite markers in genotypes of horse.
We compared the genetic diversity and distance among nine horse breeds Czech and Slovak Warmblood both of Czech origin, Slovak Warmblood of Slovak origin, Hucul, Hafling, Furioso, Noriker, Silesian Noriker and Bohemian-Moravian Belgian Horse.
In total, 932 animals were genotyped for 17 microsatellites markers (AHT4, AHT5, ASB2, HMS3, HMS6, HMS7, HTG4, HTG10, VHL20, HTG6, HMS2, HTG7, ASB17, ASB23, CA425, HMS1, LEX3) recommended by the International Society of Animal Genetics.
In the different population size, the allele frequencies, observed and expected heterozygosity, test for deviations from Hardy-Weinberg equilibrium and Polymorphism information content have been calculated for each breed. We analyzed genetic distance and diversity among them on the base of the dataset of highly polymorphic set of microsatellites representing all autozomes using set of PowerMarker v3.25 analysis tools and Structure 2.2. programme for results comparison.

The enzyme 4-hydroxyphenylpyruvate dioxygenase (HPPD) has a very important role in the biosynthetic pathway of vitamin E. Its activity influences the final level of tocols in plant tissues. Seven barley cultivars with different vitamin E level were grown under control conditions and activity of HPPD gene was measured four, eight and twelve days after pollination of ear tissues. It was found that activity of HPPD gene corresponded with vitamin E content detected in grains (r = 0.77*). The relationship between the gene activity for HPPD eight and twelve days after pollination and vitamin E content was also confirmed for analyzed cultivars grown in the field conditions (r = 0.85*).

This paper presents an approach for developing the presentation layer of software applications. The approach is based on the concept of the Model Driven Architecture (MDA) and uses a UML - based model of graphical user interfaces, which is created according to rules defined in a meta - model. The meta - model is not oriented to a particular platform, thus all designed models can be created independently of the programming language and widget library. This platform independent UML based model can be transformed into source - code for an arbitrary programming language and can be used in a software development process.
The meta - model of our approach is an extension of the common UML and provides support for modeling the presentation layer. The meta - model thus fills a gap that exists in modeling three - layered software applications, beside the application and the data layer. By providing this possibility for modeling the presentation layer, we can crucially impact current approaches to the development of three layered software applications. All model artifacts contain essential information about the graphical user interface and can be used for a code generation. Since the UML is widely used by analysts, they can produce models which de-facto represent source code and thus they reduce the workload for programmers, who create source code by some traditional approaches. Our model - based approach also strictly separates the appearance and the structure of graphical user - interfaces and both of them are developed separately, which brings higher modularity of software.
In this paper, we demonstrate our development approach by focusing on the structure of graphical user interfaces. Our approach is influenced by the concept of Model Driven Architecture and we deal with all related issues, such as meta - model, user models, model transformations and source - code generation. For evaluating our approach, we designed and developed a software framework, we integrated it into a generic modeling tool, and used approach principles during the development of a module of an information system.

This contribution investigates the problematic of indoor air pollution. There were tested low emitting types of lacquers - UV and waterborne lacquers. The work has been splitted in to two parts. In the first part samples of lacquers were applied on the particle board with beech veneer on the surface. Samples of air with VOCs (Volatile Organic Compounds) from lacquers were taken 3 and 24 hours after application. Tests proved that film layer of UV lacquer emit much smaller volumes of VOCs than acrylic waterborne lacquers or than 2-component epoxide waterborne lacquer. Acrylic waterborne lacquers' materials emitted especially butoxyethylene, epoxide lacquer emitted a lot of butoxyethanol and methoxypropanol and UV lacquers particularly emitted toluene, benzene and xylene. Second part of testing consists of VOCs' measuring of chosen lacquers applied on different base materials - inertial material glass and particle board with beech veneer. Tested criterion was the impact of different base material on emitted amount of VOCs. The results showed that lacquer coated on glass emit much more chemicals than lacquer on wood material. All materials were also tested on total amount of organic compounds emitted in to the air - on the TVOC. Measuring showed the same results as the first parts of research.

Grape phylloxera, Dactulosphaira vitifoliae Fitch., is one of most destructive insect pest of cultivated grapes (Vitis vinifera L.) worldwide. Phylloxera was the cause of incomparable structural changes in European viticulture. It also led to the usage of new biotechnology - rootstocks for grape varieties. The direct damage is related to phylloxera feeding of roots and leaves. Rootstocks bred solely with American Vitis spp. parentage allow little or none of phylloxera related root damage that is seen on Vitis vinifera. Rootstocks with partial Vitis vinifera parentage may also confer a high level of grape phylloxera resistance, but this resistance is not durable. Rootstock was evaluated under laboratory conditions and with pot trials. Resistance against phylloxera at roots and leaves was evaluated. Highest resistance degree was proved for variety Börner. Good resistance was found for most of tested rootstock varieties. Very good resistance have SO4 PO 0/7 and Craciunel 2 PO 0/6. It is interesting, that hybrid Kl×SO4 has also relatively good resistance. This promising franco-american needs further examination thanks to its good growing properties. After long time, this research offers new results about rootstock and phylloxera interactions under conditions of Southern Moravia. Results serve as a ground for further resistance breeding against phylloxera in Czech Republic.

During the years 2001 and 2002 we conducted hydrochemical monitoring of intensively managed pond to evaluate the impact of high pig slurry doses on eutrophication. Pig slurry application was carried out in colder period of the year (February-April) via tube system with sludge pump from nearby piggery.
Our results showed that pig slurry application do not permanently affect the water quality of Jarohněvický pond. When the correct application is made slurry is effectively utilized by biomass for the growth, which prevents surface and underground waters to be polluted as in the case of incorrect application on agricultural land.
Critical point of this technology in terms of water management is the way of pig slurry application and the exact dose. Even extremely high slurry doses (16.1 kg.m^-2 in 2001 and 15.6 kg.m^-2 in 2002) used in Jarohněvický pond did not negatively affected pond ecosystem. Only higher amount of organisms that increased natural fish production was recorded. It is necessary to implement this ameliorative intervention in colder period of the year considering higher hazard of variations in decisive hydrochemical parameters at higher water temperature.

Essential part of raw milk component measurement is indirect infraanalyse. The checking of time measurement stability is important there. The preparation of pilot samples was described. Information about deep frozen milk pilot sample stability are sporadic. Aim of this work was to verify the stability of long-term stored and deep frozen pilot samples (FPSs). Pilot samples were frozen in liquid nitrogen bath -196 °C and after that stored at -21 °C for one month (A) and more months (B) till analyse. The pilot samples were measured by CombiFoss 6000: fat F (%); protein CP (%); lactose L (%); casein C (%); solid non fat SNF (%); urea U (mg/100ml); somatic cell count SCC (ths./ml). In the short-term (A) experiment the impact of freezing on pilot milk samples was relatively small, in the framework of acceptable value of repeatability (±0.02 %) for components. The stability of values of FPSs was better according to standard deviations for important F evaluation in H (Holstein) breed samples. The repeatability of values of FPSs in the time was very good for other components. The repeatability was better for SCC in J (Jersey) breed samples (±16.5 thousands/ml). That is why the higher variability (±30.1 thousands/ml) in H sample is visible less sceptically. The apparent result trends were not observable in the repeatability development of important milk indicators in FPSs. The mild trend was only in CP, but this trend covered absolutely very small shift (variation range). There are only oscillations, which are similar between J and H samples. It is possible to attribute these oscillations rather to instrument effects than to sample effects. The oscilations were markedly higher in the long-term (B) experiment. Between J and H samples the oscilations were very similar in curves F and L. It is also possible to attribute them more to instrumental effects. The repeatability values (standard deviations sd) of FPSs were mostly lower than ±0.06 %. It is acceptable for result agreement between indirect and reference method. Relevant sd varied from ±0.025 % for L to ±0.059 % for F (in J breed) with exception CP ±0.085 % in J sample. Also B procedure was shown as applicable for instrumental sability control in half year period. In practice the one reference value for concrete FPS is valid from one to next calibration. Persistent trend in repeatability of FPSs can indicate an inadequate instrumental drift. The applied FPS procedure is usable for stability control of instrumental measurement in milk laboratories.

In 2003-2005 it was solved the issue about the nutrient balance in the permanent grasslands of Research Institute for Cattle Breeding Rapotín. In trial it was used these types of utilization: 1. Intensive - 4 cuts per year (1^st cut up to 15^th May, every next after 45 days), 2. Medium intensive - 3 cuts per year (1^st cut from 16^th to 31^st May, every next after 60 days), 3. Low intensive - 2 cuts per year (1^st cut up to 15^th June, 2^nd after 90 days), 4. Extensive - 2 cuts per year (1^st cut from 16^th to 30^th June, 2^nd after 90 days). There were following variants of the fertilization: A - no fertilization, B - P:K 30:60 kg.ha^-1, C - N:P:K 90:30:60 kg.ha^-1, D - N:P:K 180:30:60 kg.ha^-1. There was the considerable reaction of the permanent grassland on the nutrient application. The highest value of the coefficient of the neutral efficiency was by the fertilization N:P:K 90:30:60 kg of the pure nutrients. By the most of the variants we have noticed the negative nitrogen balance. The negative balance of the phosphorous was marked only by the unfertilized variants. All variants of the permanent grassland utilization had the negative potassium balance. The utilization had the significant influence (P_0.01) to all observed indicators. The year had the significant influence too. From the point of view of the rational plant nutrition and of the environment protection from the contamination (especially from the nitrates) it appears as the most advisable the medium intensive type of the utilization with nitrogen dose: N:P:K 90:30:60.

The effect of known CSN3, Pit-1 and LGB genes single nucleotide polymorphism (SNP) on milk performance traits (milk yield, protein, fat and lactose content, count of somatic cells) at the Czech Fleckvieh and the Holstein cattle breed was estimated. For more exact results, tested cows of each breed came from one source, and were selected into two groups according to their lactation. We tested around 100 animals at the first and 25 at the high (more than five) lactation of both breeds. Our attention was aimed particularly on combined genotypes of favourable alleles. We confirmed the positive tendention of the cheese yield valuable CSN3/BB with Pit-1/AA combined genotype for high milk production. By estimation of single gene effect, we found out significant association (P ≤ 0.01) between milk yield (kg) and Pit-1 genotypes at Holstein cows with the high lactation. This dependence was observed at the Czech Fleckvieh breed too, even it didn't get conclusive value. Among all tested individuals, CSN3 genotypes significantly influenced milk yield (AA > BB, P ≤ 0.01), milk fat (BB > AA, P ≤ 0.01) and protein content (BB > AA, P ≤ 0.01). The genotypes AB and BB of the LGB gene at Holstein cows were joined with higher milk yield in selected cattle groups opposite to AA genotype (P ≤ 0.01, P ≤ 0.05). The influence of LGB on the somatic cell count in created cattle groups was not confirmed. Estimation of allele and genotype frequency according to lactation stage didn't reveal higher differences at non of tested genes in both breeds.

Global shape of the peaches of the Red Haven variety (harvested on different days during July, 2007) has been characterized by calculating the H/Ds, H/Dc and Dc/Ds ratios. There is no, or nearly no, differences between peaches harvested at the different days. The shape of the all tested peaches was very close to round one. The more detailed description of the peach shape is based on the Elliptic Fourier analysis (EFDs) of the digital photos. The knowledge of the parameters (curvature etc.) is very useful e.g. for the evaluation of the results of the strength tests. Compression tests have been also perforemd - the whole peaches have been compressed between two plates at the constant cross-head velocity 20 mm/min. The force-displacement curves are characterised by certain monotonic increase and the point called bioyield, where force exhibits a drop. The bioyield significantly decreases with the date of the harvesting. There is no evidence of the dependence of this parameter on the direction of loading. The same result is approximatively valid for apparent modulus of elasticity E. The force-displacement curves have been successfully modelled by non-linear mathematical models.

The ripened cheese is typical by taste and aroma and texture also. The object of research was the influence of ripening on sensory and texture properties of Olomouc cheese with the determination the optimum lenght of ripening for consumers. High storage temperature of curd, deficiency of air oxygen and some other factors determine the senzory characteristics of this type cheese. The samples were taken from the factory directly and stored in the same conditions in cold (7 °C) during 5 weeks. Aroma, colour, appearance, level of ripening, consistence, taste, dry matter and texture analysis by TIRA machine 27 025 were followed. Cutting test measured the resistence to the passage of a knife, penetration test involved measurement of the force required to insert a probe a given distance into cheese. The best results of the flavour have been observed in three and four weeks storing. The best ripening and consistence were evaluated in the fourth and fifth week after cheese production. There was found the decrease of the cheese firmness during the ripening.

Educated university graduates are demanded by employers. Requirements of employers on graduates are high; most required are communication skills, decision-making skills, problem solution skills. Very often demand is graduates' willingness to further education, knowledge of foreign languages. The paper describes the opinions of students of Faculty of agronomics and Faculty of business and economics MUAF in Brno on the possibilities to deepen selected skills. Students of both faculties realize the necessity of further training. More than 96% of students consider the knowledge of foreign languages to be very important and prefer teaching grammar and conversation. Students evaluated the quality of teaching foreign languages at MZLU in Brno, too. Almost 40% of students of Faculty of agronomics and 70% of students of the Faculty of business and economics consider the teaching of foreign languages as insufficient. These negative views were probably influenced by a small extent of teaching languages during their studies. Communication skills represent another significant factor, and students evaluated the possibility to develop these skills, as well. 50% of respondents from the Faculty of agronomics evaluate these skills as necessary for practice and positively evaluate the development of these skills within teaching at MUAF in Brno. Positive evaluation of communication skills development was also found nearly in three quarters of respondents from Faculty of business and economics.

Resistance to lime-induced chlorosis is very important characteristic of grapevine rootstocks. Lime-induced chlorosis influence yield and quality of grapes. Evaluation of chlorosis resistance is very important for grape growing and breeding. The aim of this work is provide results of evaluation grapevine rootstocks to chlorosis. High resistance was show in Fercal. From the viewpoint of the resistance to chlorosis, the rootstocks registered in the State Variety Book of the Czech Republic can be ranked from the most resistant to the most sensitive as follows: Craciunel 2 - SO 4 - Kober 125 AA - Kober 5 BB - Teleki 5 C - Amos - LE-K-1.

The effect of N and P fertilization on the dry matter content, concentration of nutrients (N, P, K, Ca and Mg) and their intake by the plant, production characteristics (achene yields, head diameter, 1000-seed weight, oil content and oil production) and the content of fatty acids (palmitic, stearic, palmitooleic, oleic, linoleic and linolenic) was evaluated in the one-year pot trial with sunflower (Helianthus annuus, L.). Nitrogen application had a positive effect on dry matter production and increased N intake by plants as early as the beginning of vegetation (4^th true leaf). The effect of P on dry matter production was not markedly evident until the later growth stage (10^th true leaf). At the end of the vegetation period the weight of plants of variants fertilised with N only and variants where P was applied evened out. Achene yields, head diameters, 1000-seed weight, oil content and oil production increased statistically significantly after N application. Of all the monitored characteristics the increased level of P in the soil affected only the 1000-seed weight. The application of both nutrients did not significantly change the content of fatty acids.

Identification of improvement areas and utilization of information and communication technologies have gained value and priority in our knowledge driven society. Rules define constraints, conditions and policies of how the business processes are to be performed but they also affect the behavior of the resource and facilitate strategic business goals achieving. They control the business and represent business knowledge. The research works about business rules show how to specify and classify business rules from the business perspective and to establish an approach to managing them that will enable faster change in business processes and other business concepts in all areas of the business. In concrete this paper deals with four approaches to business rules formalization, i. e. notation of OCL, inference rules, decision table and predicate logic and with their general evaluation. The article shows also the advantages and disadvantages of these approaches of formalization. They are the example of every mentioned approach.

Series of mechanical tests (uniaxial compression test, Brasil test, plate bending test) with cylidrical specimens made from dark chocolate have been performed. The stress-strain dependence has been ploted and modeled using data from compression test. The strain behaviour of the chocolate was found to be far from the linear elastic strain. The linear elasticity is limited for the very small strains. The Brasil test led to the development of the tensile stress in the specimen and its cracking. The dependence of the tensile stress on the strain rate has been confirmed. Also the linearity of the force vs. displacement data received during plate bending test has been limited to the very small value of the displacemnt. Impact behaviour of the chocolate has been also evaluated. Falling of the specimen from defined hight led to its fracture. This experiment has been simulated using LS DYNA 3D finite element code.

One of the most important parts in a precision farming system is the crop yield mapping. The pre-processing of the point data from combine harvesters, the transfer of the yield data onto the management grid and the interpolation of the yields are discussed, as well as using of the yield data themselves. The goal of the contribution was to verify, with the help of the field measuring on two stations, the dependence of the yield obtained by processing of the measured yield data from the combine harvester (where the yield monitor is installed) on the weighted yield. This is because of yield variability, which is the basic dependent variable of majority belonging to the rest kinds of field variation that provides the basic information about the field. Afterwards it is possible to transfer the yield data as the base for the variable application of the fertilizer. By this we access yield effects for the different site - specific treatment.

Intensity of snowmelt erosion was computed for cadastral areas of Pohořelice, Strážnice, Kuchařovice, Holešov, Dukovany, Vizovice, Velké Meziříčí and Bystřice nad Pernštejnem on the base of 1980-2006 data for the period November to March. Mean long-term soil loss was estimated according to ZACHAR (1981) equation on the base of average rate of snowmelting, amount of melting water, outflow and infiltration characteristics, topographical factor, soil factor and vegetation factor. For the studied localities the average rate of snowmelting varied from 2.29 mm.day^-1 to 7.90 mm.day^-1. Also amount of melting water reached the maximum in Bystřice nad Pernštejnem in dependence on climatic region (6.11 cm of water column). Outflow and infiltration characteristics varied from 0.94 to 1.14. High value of topographical factor (10.5) was assessed in Vizovice. Soil factor was estimated according to main soil unit from Estimated Pedologic-Ecological Unit EPEU. Its values varied from 0.31 in Velké Meziříčí to 0.47 in Holešov. Vegetation factor varied from 0.2938 in Vizovice to 0.4881 in Kuchařovice. Average soil loss as a consequence of snow melting varied in interval from 0.61 t.ha^-1.year^-1 in Pohořelice to 30.08 t.ha^-1.year^-1 in Vizovice.

The objective of the present study is to characterize the rheological properties of peaches according to the results of puncture test force/displacement curves. The measurements were carried out on the universal testing machine. The firmness of the peaches was measured on the equatorial side of the fruit. Four measurements were carried out on each fruit by varying the orientation at intervals by about 90°. The used approach computed six texture parameters from the curves, which were supposed to be representative of skin hardness, fruit deformation before skin rupture, flesh firmness and mechanical work needed to penetrate the fruit. The data were fitted by an exponential function. The correlation is not statistically significant. Its value only support the hypothesis about decrease of the skin strength and other values. Generally, there is a decreases with time from the harvest beginning but the scatter of the data is too high. The results show that used method enables to describe the peach firmness development during the harvest period but the effective use of this method is based on the large number of experiments which are destructive in their nature. Some new approach is briefly outlined.

This paper is focused on validation of new synthetic raw cow milk quality indicator which was calculated according to previously developed algorithm. The aim was to carry out a synthesis of values of individual milk quality indicators (MQIs) into one standardized relative milk quality indicator (SQSM). This is useable for farmer milk price modification according to milk quality in dependence on its change in every case. It is a possible ground for consistent milk payment according to its quality. This work is fifth step in the row of works. It is carried out in terms of next promotion of the milk foodstuff chain safety and for the improvement of the ability of the dairy production branch for the competition. SQSM was calculated according to formula as follows: DX = (IND - x)/sx, where: IND is individual value of MQI of supplier, x is month arithmetical average of MQI of relevant milk suppliers including IND and sx its standard deviation; SQSM is the sum of logically oriented (according to milk quality growth) DXs, which come from relevant MQIs of identical raw milk delivery; SQSM as sum can be also divided by the number of MQIs. It means, there are possible two kinds of expressions of SQSM, sum (a) and/or average (p). The values of MQIs were used in original (compositional and physical MQIs) or transformed (log; hygienic MQIs) form according to previous research results. Three, five and seven MQIs and single, double and threefold values of their technological weight were included into model validation as price formula. Thirteen variants (p and a) of price formula were used, too. The behaviour of p and a model variants was similar. All SQSM averages and medians were very near zero value. The normality of SQSM files was reached (P > 0.05) sometimes in obliquenes (a₃) which is more important in this case. The normality was not reached in acuteness (a₄). The SQSM model files were quite well balanced in terms of positive and negative values. All model combination differences (p) between variants were insignificant (calculated F 1.76×10^-18, where P > 0.999; critical F 1.75 for P ≤ 0.05). The difference insignificance was independent both on number of MQIs and on their technological preference as well. The results of SQSM validation showed the suitability of indicator usage for intended purpose of consistent milk price modification according to every milk quality change.

A two year-experiment was carried out to study the effect of root inoculation with two biopreparations, Vambac^® (VA-mycorrhiza genus Glomus, Gigaspora and the rhizospheric bacteria Agrobacterium radiobacter) and Amalgerol^® (composed of vegetative and sea-algae oils and extracts) on leaf mineral concentration, vegetative and generative behaviour in two year old apple trees cv. 'Jonagold'/M.9. Trees were grown in 10 l plastic pots in unsterilized sandy clay soil (Fluvisols) under climatic conditions of South Moravia (49°,25' N; 16°,55' E), Czech Republic. Leaves were sampled eight times during the experiment (four times per year) for analysis of: N, P, K, Ca, Mg, Zn, Fe, Cu, and Mn. Mycorrhizal infection was determined with the gridline-intersection method. Inoculated plants showed higher VA root infection during both years (best results were 46% on the first year and 41.7% on the second year) and higher leaf P concentrations, in particular when biopreparations were applied together (P = 0.23% DW), than non inoculated (4% of root infection during year one and 15% during year two; P = 0.183% DW). N, K, Ca and Mg were not significantly affected by treatments. In many cases application of biopreparations decreased Fe, Mn and Zn leaf levels. Shoot length was strongly enhanced in inoculated plants only during the first year (46% more than non inoculated). No significant differences were detected between inoculated and non inoculated plants for: trunk diameter, spur number, root volume, leaf dry weight, number of blossoms and yield. It is concluded that the studied biopreparations have the capacity to colonize and persist in the roots of apple trees (VA-mycorrhizal fungus), enhance the uptake of phosphorus and vegetative growth but they could decreased the uptake of Fe, Mn and Zn.

Demographic evolution in the Czech Republic is not spatially homogeneous but displays regional differences. In the set of 14 regions of the Czech Republic, numerical characteristics showing level, variability, skewness and kurtosis for 30 demographic indicators from the year 2006 are calculated and evaluated. Frequency counts for some of these have been shown using histograms. On the basis of factor analysis, five representative indicators were chosen and assigned weights, with the ranks of regions determined by normalized values. The best position was obtained by the City of Prague and Středočeský Region, with last position occupied by the Zlín Region.

In a small-plot trial with potatoes were applied increasing doses of selenium to the soil (0, 12, 24, 48 and 72 kg Se.ha^-1) and after harvest were explored the content of selenium, phosphorus and sulphur in raw tubers and in the tops. The trials were conducted in two localities - Žabčice and Valečov using the semi-early variety Ditta. Selenium was applied to the soil in the form of sodium selenite before planting the potatoes. Chemical analyses discovered that with the applied dose the level of selenium increased in both tubers and tops and that the correlation between the applied selenium dose and its content in tubers and tops was significant (α < 0.05) and positive (r = 0.885 and r = 0.908, respectively). With an increasing dose of selenium the reduction in the level of sulphur in the tops (r = -0.872, α < 0.05) was statistically significant. In both localities the range of the sulphur levels in tubers was very narrow, i.e. 0.16-0.18%, and the effect of the selenium dose was not significant. The phosphorus content in tubers and tops did not change significantly after application of a dose of up to 48 kg Se.ha^-1. With the highest dose of selenium the content of phosphorus in both tops and tubers decreased significantly.

The paper deals with problems concerning customers' satisfaction. It summarizes results of primary research focused on an analysis of fundamental factors, which shape clients' preferences, influence the level of satisfaction of transportation companies' customers in general and in transportation companies under study in particular. Altogether 49 % of respondents mentioned that the type of transportation means was the most important factor that influenced their satisfaction. As other significant factors influencing the satisfaction of respondents the following were mentioned: prices of transportation, quality of services during the transportation, respecting regular pauses during transportation, and the possibility of refreshments during the transportation. All companies that were addressed monitored the level of satisfaction of their customers (nearly 20 % did this every month). Each company that monitored satisfaction of its customers every year also developed a marketing plan of their activities. The paper also contains detailed results of conducted inquiries as well as the relevant discussion.

The goal of this paper is to investigate the effect of the end distance of cam lock fasteners on the bending moment resistance of knock-down corner joints. The preliminary study of knock-down furniture assembly plans was done in order to discover the manufacturers' fastener typical usage in case construction. Laminated particleboard, cam fasteners and wooden dowels were used for specimen construction. L-shaped joint specimens 760 mm in length were tested by pressing the joint members together - also called a compression test in the angle plane. The study results showed that cam fasteners with end distance of 60 mm from the member edges perform the best.

During the vegetative period of the year 2005 (June-October) we carried out hydrobiological and hydrochemical monitoring of selected ponds inhered in Southern Moravia in relation to different fishery management intensities. Water temperature, dissolved oxygen content, pH, conductivity and water transparency were monitored directly at taking place, N-NH₄⁺, N-NO₂^-, N-NO₃^-, P-PO₄^3- content and chlorophyll-a concentration were measured in hydrochemical laboratory. At the same time, water samples for taxonomical analyses of phytoplankton and assessment of toxin content of cyanobacteria were taken. All ponds were characterized by low water transparency, high values of pH, N-NH₄⁺, P-PO₄^3- and high chlorophyll a concentration. We detected maximum concentration of 18.7 µg.l^-1 microcystins in ponds dominated by the species Microcystis aeruginosa. Total microcystin concentration in water of all ponds with dominance of pikoplanktonic cyanobacteria were below detection limit (LOD < 0.125 µg.l^-1). The dominance of cyanobacteria species shifts from coccal (especially genus Microcystis) to small-colonial pikoplanctonic (genus Aphanocapsa, Aphanothece) and to single-filamentous (genus Planktothrix, Limnothrix, Pseudanabaena) cyanobacteria related to increasing fish stock density.

Over the period of July to September 2005 effects of insecticide Integro with the effective substance methoxifenozide and biological preparation Trichoplus using parasitoid wasps Trichogramma evanescens and T. pintoi were evaluated in maize stands. These natural enemies of aphids were recorded in monitored fields (located in South Moravia, Czech Republic): parasitoids of genera Aphidius and Praon (Hymenopetra, Aphidiidae), predatory syrphid flies (Diptera, Syrphidae), ladybirds (Coleoptera, Coccinellidae), Orius bugs (Heteroptera, Anthocoridae), Green Lacewing - Chrysoperla carnea (Neuroptera, Chrysopidae) and spiders (Araneida). No significant difference (T = 0.003; F = 4.894; P = 0.004) in populations of aphids and their antagonists was found in the studied variants (Integro, Trichoplus). More remarkable differences were found only soon after spraying with Integro. Syrphid flies pupae decreased by 41% in the Integro variant, and by 13% in the Trichoplus variant. Ladybird larvae did not occur in the Integro variant after spraying, there where 0.12 ladybird larvae on plant in the Trichoplus variant. Number of ladybird adults increased from 0.01 to 0.53 individuals per plant in the Trichoplus variant, but from 0.03 to 0.1 individuals in the Integro variant. Number of ladybird pupae decreased from 0.32 to 0.02 pupae per plant in the Integro variant, but it has increased from 0.04 to 0.54 in the Trichoplus variant. Correlative relationship between ladybird adult growing coefficient and aphid populations in the Trichoplus variant was found.

Bog pine (Pinus uncinata DC. subsp. uliginosa (Neumann) Businský) is a subendemic species and appears to be one of the most endangered tree species of the Czech Republic. Its rare populations are at the present time greatly endangered namely by spontaneous hybridization with the Scots pine (Pinus sylvestris L.). Regarding the fact that its protection is insufficient even in national nature reserves (NNR) and the classical propagation by cuttings is problematic, modern methods were adopted for a long-term preservation of the taxon. Growth regulation conditions were investigated for the induction of organogenesis and somatic embryogenesis. Mature seeds were obtained from selected trees in the Žofinka NNR and from a locality near the village of Krajková in the Sokolov district. Cotyledon and hypocotyl segments from seedlings pregerminated in sterile conditions were used to induce the organogenesis. Bud proliferation was observed on the cotyledons after 4 weeks. The largest amount of buds was found on the medium with 1.5 mg.l^-1 benzyladenine (BA) and 0.5 mg.l^-1 1-naphtylacetic acid (NAA) after 12 weeks. The hypocotyl segments showed only the formation of green callus. Isolated mature zygotic embryos were used for the induction of somatic embryogenesis. Development of mucilaginous callus was recorded after 3 weeks of cultivation on media with different combinations of BA, NAA and 2,4-dichlorophenoxy acetic acid (2,4-D) concentrations. When the callus induced on the medium with the combination of 0.56 (1.13) mg.l^-1 BA and 1.86 mg.l^-1 NAA was transferred on the medium with lower BA (0.113 mg.l^-1) and 2,4-D (0.44 mg.l^-1) concentrations, the first somatic embryos started to emerge after a period of other two weeks.