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The present work gives the results of the hydrobiological research of the middle course of the Bečva River in the river section between Valašské Meziříčí and Přerov, carried out in the years 2004 to 2007. Altogether, 65 taxa of macrozoobenthos were determined in the period 2000-2007 in the middle course of the Bečva River : Coelenterata (1 taxon), Oligochaeta (5 taxa), Hirudinea (3 taxa), Gastropoda (4 taxa), Isopoda (1 taxon), Amphipoda (2 taxa), Hydracarina (1? taxon), Ephemeroptera (7 taxa), Plecoptera (1 taxon), Odonata (3 taxa), Heteroptera (1 taxon), Megaloptera (1 taxon), Trichoptera (9 taxa), Coleoptera (3 taxa), Diptera (23 taxa). The number of zoobenthos taxa fell in the period 2004-2007 in comparison to period 2000-2002 from 51 taxa to 40 taxa. The decline of zoobenthos taxa was registered on study localities, as follows : Choryně (from 28 to 23 taxa), Hustopeče (from 27 to 25 taxa), Rybáře (from 35 to 28 taxa), Grymov (from 31 to 25 taxa). Average saprobity index of benthic community of the middle course of the Bečva River was 2.35. Average saprobity indices of monitored localities towards downstream were as follows: Choryně 2.41; Hustopeče 2.25; Rybáře 2.40; Grymov 2.34.

High quality and purity of DNA isolated from food and feed is essential for species identification and has unpredictable influences an effect of analysis. In this study, the efficiency of eight different methods for DNA isolation was investigated. For DNA extraction, the raw chicken meet, ham, sausages, tinned lunch meat, pate, tinned feeds for dogs, complete granulated feeds for dogs and chicken flour were used. Kits of several different producers, i.e.: NucleoSpin Food (Marchery-Nagel), Wizard Genomic DNA Purification Kit (Promega), Invisorb Spin Food Kit I (Invitek), Wizard SV Genomic DNA Purification System (Promega), JetQuick Tissue DNA Spin Kit (Genomed), RNA Blue (Top-Bio), JetQuick Blood & Cell Culture Kit (Genomed), QIAamp DNA Mini Kit and QIAamp DNA Blood Mini Kit (Qiagen) were employed in the study. Gel agarose electrophoresis for primary verification of DNA quality was performed. The isolates were subsequently assessed for quantity and quality using by spectrophotometer Nanodrop 2000 (Thermo Scientific). To verify of template usability and quality of isolated DNA, the polymerase chain reaction (PCR) was used.
Differences between isolated DNA from tinned products and meat, ham, sausage, granulated dog feed and chicken flour were found. In tinned food and feed, the DNA was more degraded, DNA content and DNA purity was lower and also PCR amplification was the most difficult. Overall DNA yield and quality have important influence on PCR products amplification. The best results were obtained with NucleoSpin Food and JetQuick Tissue DNA Spin Kit. DNA extracted by these methods proved highest yields, purity and template quality in all foods and feeds and the results of PCR analysis are excellent reproducible. Analyses showed that results depended on different food or feed using and different isolation system.
The results of this work will be utilized to choose the suitable isolating kit for educational course, which is designed for students and also for following research and analyses.

The orientation of the research work for the exploitation of the preventive tools for the decrease of biodegradable waste comes out from the requirements, which are reposed on Czech Republic as a valid member of European Union. The Czech Republic have to follow the legislative requirements, which are defined for the waste treatment, in this case it deals with the EC Landfill Directive (1999/31/ES). The directive undertakes for the EU members to limit the volume of the biodegradable waste on the landfills. The main sense of this restriction is to reduce the volume of the emitted gas, mainly methane, into atmosphere. Therefore, it was assigned the Waste Management Plan of the Czech republic, which states in the interest of the strategic goals (the decrease of the specific waste production independently on the level of the economic growth, the maximal waste exploitation as a reserve of the primary natural resources and the minimalization of negative impacts on human health and environment by the waste treatment) the goal achievement in its binding part, what's the decrease of maximal volume of the biodegradable municipal waste deposited in landfills, thus the rate of this element will be the most 75% weighted in 2010, the most 50 % weighted in 2013 and the most 35 % weighted from the total volume rised in 1995. One of the ways, how to achieve the required reduction of the waste volume deposited in the landfills, is consistent exercitation of the preventive methods and the sound agricultural and sound operating practice methods. The main goal of the work consists in creation of methodics for the make prognosis of the region development charging by the biodegradable waste in the preventive tool exploitation. I identified the typical sources of biodegradable waste and the key areas of their uprise after the evaluation of environmental gains of the selected preventive projects by the creation of methodics. After this manner of data acquirement, I proposed and defined the indicators, which serve for the creation of model database of environmental gains in the regional measure, especially collection area of landfill.

At the time of increasing globalization and homogenization of the global markets, the development of information infrastructure technology and world market becomes accessible not only to large multinational companies but also for small and medium-sized enterprises (SMEs). This has resulted in increasing competition in the domestic market which domestic firms have to face. Therefore the process of internationalization of business is a step that allows businesses performing their activities in the domestic market to enter foreign markets and to exploit the opportunities and potential which these markets offer. The paper is focused on the draft of the method for quantification of the factors, which determine the success of the SMEs in foreign markets. In the proposed method the multidimensional assessment of indicators of success of SMEs in foreign markets are used, ie. not only the proportion of sales from exports is observed, but also the profitability of international activities in an absolute and in a relative rate. Other indicators of success are the satisfaction of the company management with international activities and the success of achieving defined targets for business activities in foreign markets. Evaluation is made up of both the objective and the subjective indicators of success. Among the objective indicators of success is included the intensity of international activities of SMEs, which is detected as the sum of revenues generated from international activities in relation to total sales company, the profitability of international activities, (ie. whether the foreign activities are profitable or not), and the relative profitability of international activities, where it is ascertained whether the foreign activities generate higher profit than the activities at the domestic market. The next subjective indicator of success is the success of objectives - it was found how many objectives related to operations in foreign markets has been achieved. Another indicator is the subjective satisfaction of the company management with existing activities on foreign markets. Overall performance on foreign markets of the company is quantified using an assessment of objective and subjective indicators of success. Using the defined evaluation it will be possible to determine which of the SMEs are more and which are less successful in foreign markets. Finally, this evaluation will be used for future research aimed at identifying characteristics affecting the success of SMEs in the foreign markets.

The aim of this paper is to present results of the research focused on the examination of differences of selected psychological characteristics between a group of 144 employed people and a group of 121 unemployed people. The following personality characteristics were measured and compared: action versus state orientation (behaviour control (resp. behaviour regulation) after the experience of failure and in the processes of decision-making and behaviour planning), perseverance in the conception of Sense of coherence and social support. We found out that after having lost their job, people of higher education could better plan their decision-making and actions and they had a better ability to cope with an actual situation (Sense of coherence). The research further showed that females were unemployed more often as compared with males and that the loss of job had a worse effect on their mental state. Compared with unemployed males, the females were more experience-and feeling-oriented and their Sense of coherence showed more prominent decline. Unemployed people had a lower level of action orientation after previous failure than employed people. This conspicuous decrease of orientation to action is given apparently by the loss of the job itself and not by the duration of unemployment. The number of people composing the social support decreases with the increasing age of males. Of all the researched predictors, which were employment/unemployment, duration of unemployment, gender, education and age, gender acts (co-acts) most on the selected personality characteristics - action versus state orientation, Sense of coherence and social support.

The paper deals with the conception of the professional manager. In the current Czech management environment the terms of professionalism and professional manager are not defined, understood and respect properly. The paper was written on the basis of a primary research among 112 middle and top managers in the Czech Republic.
The beginning of the paper focuses on the introduction of the method used for the research and then follows the evaluation process of the respondents' answers. These answers were noticed and transformed into the electronic form for the subsequent evaluation process, which is described in detail in the results part. The categories were stated on the basis of recurrence of the characteristics in the answers, and the frequency of these characteristics was measured.
The outcome of the paper is the specification of the term 'Professionalism' and the characteristics of the term 'Professional Manager' by above mentioned research. The research results are discussed with the literature review in the concluding part, and the opportunities for the further development are given.