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Využití morfologických deskriptorů a DNA analýzy pro detekci duplicit v české kolekci genetických zdrojů papriky

Helena Stavělíková, Pavel Hanáček, Tomáš Vyhnánek

Acta Univ. Agric. Silvic. Mendelianae Brun. 2010, 58(1), 191-198 | DOI: 10.11118/actaun201058010191

The pepper (Capsicum annuum L.) is very popular annual vegetable either for fresh consume either as spice after drying and grinding. The fruit contains high amounts of vitamin C, provitamin A, E, P (citrin), B1 (thiamine), B2 (riboflavin) and B3 (niacin). Crop Research Institute (CRI), Department of Vegetable and Special Crops, Olomouc, the Czech Republic is the holder of the collection of pepper genetic resources. The collection of pepper consists of 504 accessions, currently. It is necessary to find duplications within collection for effective work with genetics resources. For analyses totally 41 accessions were chosen. These were divided into ten groups according name: 1. Astrachanskij, 2. Aufrechte Cayenne, 3. Bogyisloi, 4. Hatvani, 5. Japan Hontakka, 6. Japan Madarszen, 7. Kalocsai Fuszer (Edes), 8. Konservnyj Belyj 289, 9. Tetenyi and 10. Vinedale. Two approaches were used for the detection of duplications - morphological description and polymorphism of DNA. The accessions were characterized for 54 morphological traits: 1 character in seedlings, 8 characters in the plants, 10 characters in leaves, 10 characters in flowers and 25 characters in fruits. The polymorphism of DNA was analysed using the SSR (Simple Sequence Repeats) method with 8 SSR markers (Hpms 1-1, Hpms 1-5, Hpms 1-168, Hpms 1-172, Hpms 1-274, Hpms 2-21, Cams 163 and Cams 647) which are localised on different chromosomes. The results from DNA analysis were complemented with the morphological characterization. Possible duplications were in 4 groups: 1. Astrachanskij, 4. Hatvani, 5. Japan Hontakka and 7. Kalocsai Fuszer (Edes). This work is the first step for the determination of duplications in the Czech germplasm collection of pepper.

ADMINISTRATIVNÍ NÁKLADY MAJETKOVÝCH DANÍ V ČESKÉ REPUBLICE

Břetislav Andrlík

Acta Univ. Agric. Silvic. Mendelianae Brun. 2010, 58(6), 13-20 | DOI: 10.11118/actaun201058060013

The paper deals with the efficiency of property taxes in the tax system of the Czech Republic, focusing on the administrative costs of taxation on the timeline 2005 to 2008. It contains a theoretical definition of tax efficiency, and describes the types of costs connected with taxes. From this perspective it focuses on quantifying the direct administrative costs of inheritance tax, gift tax, property transfer tax and property tax. Direct measurement of administrative costs is done by using the method called WTE staff which classifies employees of regional financial institutions in separate groups and assigns each group a specific number of employees for each reference property taxes using the conversion factors. Then it defines the total expenditure of regional financial institutions using the coefficients for a particular monitored tax and it provides administrative costs as a percentage of property tax receipts. Finally, the results of measurements indicating the proposed amendment are discussed.

Stanovení podílu biologicky rozložitelné a biologicky nerozložitelné složky hřbitovního odpadu

Bohdan Stejskal

Acta Univ. Agric. Silvic. Mendelianae Brun. 2010, 58(2), 181-184 | DOI: 10.11118/actaun201058020181

Municipal waste landfilling is the most common practice of municipal waste disposal in the Czech Republic. As a member state of the EU the Czech Republic must comply with the legislative requirements set for waste management. EU Council Directive 1999/31/EC requires member states to limit the amount of bio-degradable waste into landfill.
To achieve the objectives of the Plan of Waste Management of the Czech Republic, various methods has been proposed. Prior to the waste processing, it is necessary to know the waste material composition, and after that select the most appropriate method and procedure for waste utilization or disposal.
Therefore an analysis of graveyard waste composition has been carried out, where, by repeated measurements of samples weighing more than 500 kg (the total amount of analyzed waste was 3107 kg), it was found out that the graveyard waste consists of almost 77 % of bio-degradable matter. It is operationally impossible to separate bio-degradable matter from non-bio-degradable materials. Therefore it is desirable to collect compostable cemetery green waste separately from the waste coming from the decoration of gravestones that may be energetically utilized.

Ověření modifikovaného referenčního vzorku mléka ve smyslu jeho vhodnosti pro kalibraci metody infračervené analýzy pomocí hodnocení fyzikálních vlastností

Oto Hanuš, Václava Genčurová, Tomáš Kopec, Tao Yong, Libor Janů

Acta Univ. Agric. Silvic. Mendelianae Brun. 2010, 58(2), 101-110 | DOI: 10.11118/actaun201058020101

Routine milk analyses using the efficient indirect infra-red method are important for the milk food chain quality. The reliability of the results depends on the calibration quality. It is important to use a relevant set of reference calibration samples (RCSs). RCSs with right range of values can be prepared using various methods. This paper was aimed to balance the impacts of dilution for decrease of main components in RCSs because of minimal change of matrix interference effects. Cow milk samples (MSs) were diluted (4/1) using distilled water, NaCl solution and a solution with specific composition (SC; because of disturbance in the balance of the milk matrix (NaCl 1.145; KCl 0.849; K2HPO4 1.8463; citric acid 1.7; urea 0.3 g/l)) for reduction in main milk components. Fat (F), crude protein (CP), lactose (L), milk freezing point (MFP), osmolality (OS) and electrical conductivity (EC) were measured in all (original as well as modified) MSs. The lowest MFP and OS were in the original milk -0.5559 °C and 274.5 mOsmol/kg. The MFP was increased to -0.4369 °C and osmolality decreased to 217.83 mOsmol/kg by the addition of water. The MFP was decreased (-0.4903 °C) and returned to original milk value by the addition of NaCl solutin. MFP was -0.4788 °C due to SC addition. The decrease was less than for NaCl. The ability of other SC components (K2HPO4, KCl, citric acid and urea) to MFP decrease is less than for NaCl solution. EC was highest for NaCl set 4.69 mS/cm, EC for SC was 4.48 mS/cm (P < 0.001). The original MSs set showed EC 4.27 mS/cm. The SC was the nearest to original MSs in terms of total mineral composition. ECs for both modifications differed (P < 0.001) from original MSs. The procedure is applicable for balance of interference effects of milk matrix because of relevant calibration.

Evaluace binárních klasifikačních úloh v ekonomické predikci

Martin Pokorný

Acta Univ. Agric. Silvic. Mendelianae Brun. 2010, 58(6), 369-378 | DOI: 10.11118/actaun201058060369

In the area of economical classification tasks, the accuracy maximization is often used to evaluate classifier performance. Accuracy maximization (or error rate minimization) suffers from the assumption of equal positive and negative error costs. Furthermore, accuracy is not able to express true classifier performance under skewed class distribution. Due to these limitations, the use of accuracy on real tasks is questionable. In a real binary classification task, the difference between the costs of positive and negative error is usually critical. To overcome this issue, the Receiver Operating Characteristic (ROC) method in relation to decision-analytic principles can be used. One essential advantage of this method is the possibility of classifier performance visualization by means of a ROC graph. This paper presents concrete examples of binary classification, where the inadequacy of accuracy as the evaluation metric is shown, and on the same examples the ROC method is applied. From the set of possible classification models, the probabilistic classifier with continuous output is under consideration. Mainly two questions are solved. Firstly, the selection of the best classifier from a set of possible classifiers. For example, accuracy metric rates two classifiers almost equivalently (87.7 % and 89.3 %), whereas decision analysis (via costs minimization) or ROC analysis reveal different performance according to target conditions of unequal error costs of positives and negatives. Secondly, the setting of an optimal decision threshold at classifier's output. For example, accuracy maximization finds the optimal threshold at classifier's output in value of 0.597, but the optimal threshold respecting higher costs of negatives is discovered by costs minimization or ROC analysis in a value substantially lower (0.477).

STRUKTURA PRACOVNÍCH ČINNOSTÍ ZDRAVOTNÍCH SESTER V MANAŽERSKÝCH FUNKCÍCH

Tomáš Kotrba

Acta Univ. Agric. Silvic. Mendelianae Brun. 2010, 58(3), 91-98 | DOI: 10.11118/actaun201058030091

Article includes results of work studies that were done by autoscreening method in the regional hospital in the South Moravian Region by 30 head nurses and 26 department nurses. There were gained and analyzed 544 autoscreenings working day shots in total. There was found that department nurses in this hospital in average mostly perform managerial and personal activities. From the scope of structure of managerial activities the highest proportions have the organizational activities of department in the case of department nurses. On the other hand deparment nurses plan and perform personal activities with the smallest frequency. Head nurses perform qualified nursing activities in most cases, even though as managers on the middle level of management this should not be their main activity. Both head nurses and department nurses deal with administrative issues in average more than 100 minutes per day.

Obsah kyseliny fytové ve vybraných druzích luštěnin

Petra Vojtíšková, Stanislav Kráčmar, Ignác Hoza

Acta Univ. Agric. Silvic. Mendelianae Brun. 2010, 58(1), 217-222 | DOI: 10.11118/actaun201058010217

The aim of this study was to determine the content of phytic acid (phytate) in soybeans (Glycine max), yellow shelled peas (Pisum sativum) and lentil (Lens esculenta). Dry seeds were grounded to a fine powder. The moisture of samples was determined according to the Official Journal of the European Union (2009). The moisture of the samples was 8.42% in soybeans, 11.19% in yellow shelled peas and 10.07% in lentil. The content of phytic acid was determined by the modified Holt's method (1955) using the spectrophotometer. Standard curve was measured using the Na phytate standard solution (0.2 mM). The phytate content in G. max varied from 1.28 to 1.86% in dry matter and from 1.17 to 1.70 g per 100 g of the sample. The content of phytate in P. sativum ranged from 0.49 to 0.86% and from 0.43 to 0.77 g per 100 g of the sample. The phytate content in L. esculenta varied from 0.45 to 1.39% in dry matter and from 0.40 to 1.25 g per 100 g of the sample. These obtained values could be influenced by many factors, e.g. climatic conditions, location, variety, etc.

MATERIÁLIE K FORMOVÁNÍ STUDENTŮ VYSOKÉ ŠKOLY

Rudolf Šrámek

Acta Univ. Agric. Silvic. Mendelianae Brun. 2005, 53(3), 327-340 | DOI: 10.11118/actaun200553030327

University's pedagogic deals with both parts of forming of university student, his naturing and his education. There does not marginalize method of education by traditions at the same time. Selection of materials for usage of this method is presented in article. This article goes in for life, works and heritage of founder of Agricultural University in Brno - Edvard Reich (1885-1943). Knowledge of University's pedagogic is applied into surroundings of Mendel's Agriculture and Forest University in Brno.

VLIV ZPŮSOBU A DÉLKY SKLADOVÁNÍ NA VLHKOST A SENZORICKOU JAKOST TRVANLIVÝCH SALÁMŮ

Hana Šulcerová, Jiří Štencl, A. Šulcová

Acta Univ. Agric. Silvic. Mendelianae Brun. 2008, 56(4), 183-196 | DOI: 10.11118/actaun200856040183

Heat-treated salamis "Vysočina" were produced with standard way in a meat factory; their diameter was 55 mm. Samples were stored under laboratory conditions at different temperatures: 5, 10, 15, 20, and 25 °C and sensory analysed every week during one month storage. The dry matter (d.m.) was measured, too. Descriptors of general appearance, sausage casing, texture, cut surface, dry edge, smell, taste, and salty were monitored. Biggest changes were in descriptors general appearance and sausage casing (P < 0.001) and also in dry edge (P < 0.010) during the month period. Germs of moulds were found only at 5 and 10 °C. Rapid increase of d.m. in samples was noticeable in the first week of the storage time. It was 3 % d.m. at 5 °C and 11 % d.m. at 25 °C. Increase of d.m. of salamis continues slowly in the next three weeks period; the total difference was about 10 % d.m. in the temperature range measured. Decrease of d.m. at 5 °C was noticed in the last week of the measurement. The difference was 3.5 % d.m. This change means that the equilibrium moisture content of the samples of salamis has been reached at the temperature 5 °C. The best sensorial quality of salamis "Vysočina" was in the storage temperature ranged from 15 to 20 °C.

In vitro regenerace a indukce polyploidie u Pelargonium × hortorum L. H. Bailey

Hana Vejsadová, Petra Kuchtová-Jadrná

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(5), 305-312 | DOI: 10.11118/actaun200957050305

The objective of this work was to induce in vitro shoot regeneration as influenced by plant growth regulators and ascertain an effective method of polyploidy induction using colchicine and oryzalin in two diploid cultivars 'Gizela' and 'Black Velvet Scarlet F1' of Pelargonium × hortorum L. H. Bailey. In both cultivars, benzyladenine (BA) significantly improved shoot regeneration compared to zeatin. The infiltration and the dip methods of explant treatment were used for polyploidy induction. Regenerants were analyzed using the flow cytometry (FCM). In 'Gizela' and 'Black Velvet Scarlet F1', 10 tetraploids on the level of 2n = 4x were found by the infiltration method. The tetraploidy was determined by the dip method in 4 shoots of 'Gizela' and 11 tetraploids were detected in 'Black Velvet Scarlet F1'.

VYUŽITÍ METODY WESTERN BLOT PRO DETEKCI PROTEINŮ SOUVISEJÍCÍCH S APOPTÓZOU NEUTROFILŮ

Petr Sláma

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(1), 97-104 | DOI: 10.11118/actaun200957010097

The aim of this study was to evaluate suitability of using Western Blot for detection of neutrophil apoptosis and neutrophil apoptosis-related proteins, respectively. Neutrophils were isolated from blood of healthy adult donors and incubated with G-CSF (granulocyte colony stimulating factor), GM-CSF (granulocyte-macrophage colony stimulating factor), ATP (adenosine triphosphate) and FMLP (N-formyl-methionyl-leucyl-phenylalanine). The neutrophils were incubated 4, 8 and 20 hours at 37 °C. In this assay, an expression of Mcl-1 (myeloid cell leukemia 1), XIAP (X-linked inhibitor of apoptosis) and gelsolin was analysed by Western Blot method. The results showed that Western Blot is a suitable method for detection of neutrophil apoptosis-related proteins and detection of neutrophil apoptosis, respectively.

VLIV PŘÍDAVKU PROBIOTICKÉHO KMENE L. casei 01 NA KONCENTRACI BIOGENNÍCH AMINŮ VE FERMENTOVANÝCH SALÁMECH HERKULES

Radka Burdychová

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(5), 41-48 | DOI: 10.11118/actaun200957050041

In this work, the influence or probiotic strain L. casei 01 (Sacco, Italy) on biogenic amines concentrations during fermentation, ripening and storage of fermented sausages "Herkules" was studied. Two amounts of probiotic culture were added into sausages, 0,25 % and 0,40 %, respectively. Negative controls without probiotic were also made. Dry sausages were taken from two different producers (A, B). Determination of biogenic amines tyramine, histamine, putrescine and cadaverine concentration was used using HPLC method with UV detection. The content of biogenic amines was monitored during fermentation (0-28 days) and storage (28-49) of fermented sausages.
In both producers, when 0,25 % of probiotic strains was added, the numbers of L. casei reached 104 CFU/g during the fermentation (0-28) and they remained relatively constant during the storage (28-49). When 0,40 % of probiotic L. casei 01 was added, the amount of L. casei cells were around 106 CFU/g during the whole fermentation and storage period.
A positive influence on the reduction of biogenic amines was observed in both producers. In negative controls without probiotic, the higher concentration of tyramine, histamine, putrescine and cadaverine was detected in comparison with probiotic sausages.
Differences between dry sausages with 0,25 % and 0,40 % probiotic L. casei 01 were also determined. In producer A, the concentration of putrescine and cadaverine in 0,40 % probiotic sausages were statistically lower in comparison with probiotic sausages where 0,25 % L. casei was applied. In producer B, the concentration of all monitored biogenic amines were statistically lower in 0,40 % probiotic sausages when compared with 0,25 % probiotic sausages.

INTERPRETACE VÝSLEDKŮ POKUSNÉ KALIBRACE PRO STANOVENÍ KYSELINY CITRONOVÉ V MLÉCE INFRAČERVENOU SPEKTROSKOPIÍ (MIR-FT)

Oto Hanuš, Irena Hulová, Václava Genčurová, Ladislav Štolc, Josef Kučera, Jaroslav Kopecký, Radoslava Jedelská, Zdeněk Motyčka

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(5), 87-102 | DOI: 10.11118/actaun200957050087

Citric acid (KC) in milk is indicator of cow energy metabolism. Milk laboratories set up KC determination. A method can be infra-red analyse (MIR-FT). The goal was to develop a relevant method for reference sample preparation for MIR-FT (indirect method, Lactoscope FTIR and MilkoScan FT 6000) calibration. As reference was used a photometric method (c; 428 nm). KC was added (n = 3) into some reference milk samples (n = 10, bulk milk). Mean value was 9.220 ± 3.094 mmol.l-1 with variation range from 6.206 to 15.975 mmol.l-1. Recovery c was from 100.8 to 120.2 %. Correlation between c and MIR-FT were from 0.979 to 0.992 (P < 0.001). These were lower in the set of native milk samples (n = 7), from 0.751 (Lactoscope FTIR; P < 0.05) to 0.947 (MilkoScan FT 6000; P < 0.001) in comparison to original values from 0.981 to 0.992 (n = 10; P < 0.001). Correlations between calibrated MIR-FT instruments were from 0.958 to 1.0 (P < 0.001). Average recovery for instruments (n = 12) was 101.6 ± 18.1 %. The mean differences between c method and MIR-FT after calibration (n = 4) moved from -0.001 across zero to 0.037 %. Standard deviation of differences was from 0.0074 to 0.0187 % at MilkoScan FT 6000 and from 0.0105 to 0.0117 % for Lactoscope FTIR. Relative variability of differences (MIR-F (filter technology) and FT) for major components fat (T), proteins (B) and lactose (L) in total and minor components KC and free fatty acids (VMK) was estimated to 1.0 and 7.2 and 34.4 %. The KC result is inferior than T, B and L superior than VMK. Autocorrelation (0.042; P > 0.05) of results demonstrated the independence of consecutive measurements. Milk preservation effect amounted 0.2323 (P < 0.001) with bronopol and 0.0339 (P > 0.05) mmol.l-1 with dichromate. It was (3.0 and 0.44 %) practically negligible, redeemable via relevant calibration. The results of proficiency testing in post-calibration period and evaluation of double transport stress effect samples (difference was -0.006 ± 0.071 mmol.l-1 (P > 0.05)) demonstrated the useability for central calibration system. Milk KC results of MIR-FT calibration were good and can be suitable for practical screening.

Srovnání metod šlechtění jetele lučního podle výnosu píce

Libor Jalůvka, Vítězslav Dostál, V. Meyer, B. Bayle, F. Lapage, O. Chloupek

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(2), 45-52 | DOI: 10.11118/actaun200957020045

Three methods of red clover (Trifolium pratense L.) breeding for forage yield in two harvest years on locations in Bredelokke (Denmark), Hladké Životice (Czech Republic) and Les Alleuds (France) were compared.
Three types of 46 candivars1, developed by A) recurrent selection in subsequent generations (37 candivars, divided into early and late group), B) polycross progenies (4 candivars) and C) geno-phenotypic selection (5 candivars) were compared. The trials were sown in 2005 and cut three times in 2006 and 2007; their evaluation is based primarily on total yield of dry matter. The candivars developed by polycross and geno-phenotypic selections gave significantly higher yields than candivars from the recurrent selection. However, the candivars developed by the methods B and C did not differ significantly. The candivars developed by these progressive methods were suitable for higher yielding and drier environment in Hladké Životice (where was the highest yield level even if averaged annual precipitation were lower by 73 and 113 mm in comparison to other locations, respectively); here was average yield higher by 19 and 13% for B and C in comparison to A method. Highly significant interaction of the candivars with locations was found. It can be concluded that varieties specifically aimed to different locations by the methods B and C should be bred; also the parental entries should be selected there.

POUŽITÍ GENETICKÝCH ALGORITMŮ PRO ANALÝZU NÁVŠTĚVNOSTI WWW PORTÁLU

Ondřej Popelka, Jiří Šťastný

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(6), 201-208 | DOI: 10.11118/actaun200957060201

The article proposes a new method suitable for advanced analysis of web portal visits. This is part of retrieving information and knowledge from web usage data (web usage mining). Such information is necessary in order to gain better insight into visitor's needs and generally consumer behaviour. By leveraging this information a company can optimize the organization of its internet presentations and offer a better end-user experience. The proposed approach is using Grammatical evolution which is computational method based on genetic algorithms. Grammatical evolution is using a context-free grammar in order to generate the solution in arbitrary reusable form. This allows us to describe visitors' behaviour in different manners depending on desired further processing. In this article we use description with a procedural programming language. Web server access log files are used as source data.
The extraction of behaviour patterns can currently be solved using statistical analysis - specifically sequential analysis based methods. Our objective is to develop an alternative algorithm.
The article further describes the basic algorithms of two-level grammatical evolution; this involves basic Grammatical Evolution and Differential Evolution, which forms the second phase of the computation. Grammatical evolution is used to generate the basic structure of the solution - in form of a part of application code. Differential evolution is used to find optimal parameters for this solution - the specific pages visited by a random visitor. The grammar used to conduct experiments is described along with explanations of the links to the actual implementation of the algorithm. Furthermore the fitness function is described and reasons which yield to its' current shape. Finally the process of analyzing and filtering the raw input data is described as it is vital part in obtaining reasonable results.

Optimalizace kvalitativní a semikvantitivní detekce geneticky modifikovaných plodin pomocí PCR

Tomáš Vyhnánek, Pavel Hanáček

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(5), 313-318 | DOI: 10.11118/actaun200957050313

For qualitative and semi-quantitative detection of genetically modified crops we selected the detection of the frequently used promoter 35S CaMV. To optimise the method we used two commercially available genotypes of maize from the company Monsanto (USA), i.e. the transgenic hybrid Bt-maize line MON810 and a genetically non-modified control (isogenic line to MON810). We tested the primers and PCR programmes described by Greiner et al. (2005) and Hernandéz et al. (2005). When applying PCR methods of detection of Bt-maize the first step was to optimise the protocol for the detection of the maize genome and detection of the specific sites of genetically modified MON810 maize. For detection of the maize genome we selected the primers IVR1-F and IVR1-R (invertase gene) which verify the presence of the maize genome by a 226 bp product. For qualitative detection of the insert of Bt-maize MON810 the primer pairs VW01/VW03 (Greiner et al., 2005) and BT03/BT04 (Hernandéz et al., 2005) were used to detect the 35S CaMV promoter. Products of the size 178 bp and 280 bp, respectively, verify its presence. Based on the results of qualitative PCR we selected the primers VW01/VW03 for semi-quantitative detection of the amount of DNA of Bt-maize. For semi-quantitative PCR we have chosen sampling of the amplification product in the 30th cycle of the PCR reaction. In the genetically unmodified control a detection limit of 1% of admixture of Bt-maize was determined when using semi-quantitative PCR. The same primers as for semi-quantitative PCR were also used for multiplex PCR but with half the concentration of primers for standard PCR. This protocol however will have to be further optimised. The presented results introduce PCR methods for qualitative and semi-quantitative detection of DNA of the genetically modified Bt-maize MON810 which can also be used for other GM crops containing the 35S CaMV promoter. It could be suitable to use these methods for the qualitative detection and/or for screening analyses of the detection of successfulness of transformation experiments.

OLFAKTOMETRICKÉ ZAŤAŽENIE OVZDUŠIA

Lucia Haraslínová

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(5), 111-118 | DOI: 10.11118/actaun200957050111

This paper describes the olfactometric assessment of volatile organic compounds emitted by coating films.
It describes the measurements of concentration, intensity, hedonic tone and character of odor caused by emissions of volatile organic compounds. VOCs' emitted in to the small-space chamber with capacity of 1 m3 were tested by dynamic olfactometric method. Paper also contains a comparison of olfactometric air pollution of two different types of coating films. The work is aimed to determine the relationships and dependencies between the measured values of volatile organic compounds obtained by the gas chromatography and reported sensation.

Analýza potenciálu úspěchu firmy z pohledu koncepce schopnosti úspěchu

Robert Zich

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(6), 343-350 | DOI: 10.11118/actaun200957060343

The success-ability conception represents a specific approach to company competitive strategy creation. Because of a different philosophy especially as regards the concept of competitive advantages, it requires a specific approach to evaluation of a company's market position from the point of view of its competitiveness. Basic evaluation includes four perspectives evaluating the profile of company competitiveness, suitability of the adopted approach, ability to develop the adopted approach and evaluation from the point of view of the benefit for the customer together with the ability of the competition to imitate the company's approach. This method has application not only in the area of company strategy creation but it can also be used when investigating the competitiveness of companies.

KONVERGENCE IFRS A US GAAP V OBLASTI VÝPŮJČNÍCH NÁKLADŮ

Hana Bohušová

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(3), 21-32 | DOI: 10.11118/actaun200957030021

An agreement from Norwalk was issued in September 2002 on the basis of negotiations of Financial Accounting Standards Board (FASB) a International Accounting Standards Board (IASB) for the elimination of restrictions in the form of two different systems of financial reporting. The aim is to ensure absolute compatibility of existing U.S. GAAP and IFRS and the coordination of work programs in the future. Document Memorandum of Understanding was issued in 2006 in the frame of ongoing process of convergence, in which the FASB and IASB confirm their intention to create high-quality and global financial reporting standards. In this document there are specified areas, which would lead to convergence of procedures.
Borrowing costs have been one from the fields, which was the subject of convergence works. U.S. GAAP dictated capitalization of borrowing costs incurred in connection with the acquisition of qualifying assets, while the IAS/IFRS leaved the decision on accounting entities, how it recognize and record borrowing costs incurred in connection with the acquisition of the assets.
In the beginning, the method of analysis and description is used to identify and describe the basic difference of both systems, which had existed before the initiation of convergence within the frame of "Borrowing Costs" project. The above mentioned primary analysis has served as the basis for the further comparative analysis and synthesis.
The paper further presents comparative analysis of two basic methodical approaches towards the borrowing costs reporting incurred in relation to the acquisition of the qualifying assets, which was possible to apply until 31st Dec 2008 under IFRS system.
The comparative analysis is based on empirical company data. The subjects are mainly the differences in the indicators, which are used for the evaluation of the company financial situation. According (Suvova, 1999), mainly the indicators of vertical analysis of property and capital structure and selected ratio indicators of return are used.
Short-term project of borrowing costs, whose aim was to unify the reporting of interest on borrowing funds on the side of IASB, was completed in March 2007 by issuing of the revised IAS 23 Borrowing Costs with effect from 1.1. 2009. The paper deals with major changes to IAS 23 occurred in the context of the IASB - Borrowing costs. Elimination of a major difference in the reporting of borrowing costs in the system IAS/IFRS and U.S. GAAP was reached by the revision of IAS 23, however all the existing differences were not removed.

Kvalita surového kravského mlieka z ekologického a konvenčného poľnohospodárstva

Juraj Čuboň, Vladimír Foltys, Peter Haščík, Miroslava Kačániová, Iveta Ubrežiová, Stanislav Kráčmar

Acta Univ. Agric. Silvic. Mendelianae Brun. 2008, 56(5), 25-30 | DOI: 10.11118/actaun200856050025

In the experiment the parameters of milk quality from organic and conventional dairy farm were analyzed. The number of somatic cells was 219. 103.ml-1 in the organic milk and 242. 103.ml-1 in the conventional milk. It seems that conditions of organic farming could be able to have a positive effect of health of mammary gland. We found the highest number of somatic cells at the end of the year (336.103.ml-1 in organic milk in December, respectively 336.103.ml-1 in conventional milk in November). The total bacteria count was higher in organic milk (86.103 CFU.ml-1) than conventional (51.103 CFU.ml-1) likewise the number of coliform bacteria. Number of coliform bacteria was by conventional milk under 1000 CFU.ml-1 for all samples. The highest number of coliform bacteria in organic milk was achieved in February (1000 CFU.ml-1). We found higher content of fat (4.23 g.100g-1) and protein (3.41 g.100g-1) by organic milk in comparison with the conventional milk (4.11 g.100g-1, resp. 3.39 g.100g-1). The higher content of protein and fat in organic milk and the higher protein content in conventional milk were determined in December. The heat resistance was determined by 96 % ethanol required to coagulation of 2 ml of milk. The conventional milk has significantly lower heat resistance (1.38 ml) than the organic one (1.86 ml). Better heat stability by organic milk and higher content of Ca (144.29 mg.100g-1) correspond with higher technological quality of organic milk.

Chování vybraných stolních tuků při dynamickém zatěžování

Šárka Nedomová

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(4), 43-54 | DOI: 10.11118/actaun200957040043

The new method of the evaluation of the mechanical behaviour of fats has been designed. This method is based on the evaluation of the fat response to dynamic loading. Dynamic loading has been realized by fall of bar impact. Response function has been represented by the time history of the fat surface displacement. Response of the tested table fats have been evaluated both in the time and frequency domain. Two factors affected rheology of the examined fat products: temperature of the measurement and product origin. The influence of fat content can be described namely in the frequency domain. The results obtained between 14 and 20 °C showed significant differences in the fat rigidity.

HODNOCENÍ ZÁJMŮ OCHRANY PŘÍRODY A FUNKCÍ LESŮ NA PŘÍKLADU CHRÁNĚNÉ KRAJINNÉ OBLASTI ČESKÝ LES

Jitka Fialová, Ilja Vyskot, Jiří Schneider

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(1), 35-40 | DOI: 10.11118/actaun200957010035

The aim of the work was to interconnect known aspects of nature conservation, which are projected in the zonation, with the quantification of forests and find out, if the interests of nature protection are in the conjuction with the high functional effectivenes of forests. For the quantification of forest functions the metod by Vyskot et al. (2003) was used. The results of quantification demonstrate that the existing zonation is one-sided and that the zonation does not fully respect the interest of nature conservation and forest function in common. According to the conflicts the new PLA Český les zonation by the disertation's method was created. The quantification is applicable not only for the new zonation but also for the preparation of foundational or management documents.

ANALÝZA HLAVNÍCH CHARAKTERISTIK "INITIAL PUBLIC OFFERING" V ČESKÉ REPUBLICE A PERSPEKTIVY JEJICH DALŠÍHO VÝVOJE

Tomáš Meluzín

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(3), 97-106 | DOI: 10.11118/actaun200957030097

Funding development of the company through the "Initial Public Offering" has a high representation globally, the Czech Republic unlike, and belongs to traditional methods of raising funds necessary for development of business in the developed capital markets. In the United States of America, Japan and in the Western Europe countries the method of company funding through IPO has been applying for several decades already. The first public stock offerings began to be applied in these markets in higher volumes from the beginning of the 60th of the last century. From that period importance of IPO goes up globally and the initial public stock offerings begin to be applied more and more even in the Central and Eastern European countries. Since 2004, several companies that have opted for this form of financing can be found in the Czech Republic as well. The objective of the paper is to analyze the main characteristics of initial public offerings of shares effected on the Czech capital market between 2004 and 2008 and to outline the perspectives of further development in this area.

Odezva Eidamu na nedestruktivní ráz

Šárka Nedomová

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(4), 55-62 | DOI: 10.11118/actaun200957040055

The behaviour of the Edam cheese during its maturation under dynamic loading has been studied. The block of tested cheese has been loaded by the impact of an aluminium bar. The force between bar and cheese has been recorded. The surface displacements as well as the surface velocities have been obtained at the different points from the point of the bar impact using of the laser vibrometers. Response functions have been evaluated both in the time and frequency domains. It has been found that the degree of the cheese maturity is well characterized by the attenuation of the surface displacement maximum. This maturation is also described by the maximum of the impact force. The spectral analysis of the response functions revealed that there was a dominant frequency, which depends only on the degree of the cheese maturity. The developed method represents a promising procedure for the continuous recording of the cheese ripening.

ANALÝZA EROZNÍCH POMĚRŮ POVODÍ VODÁRENSKÉ NÁDRŽE ŠANCE: SROVNÁNÍ VÝSLEDKŮ METOD HODNOTÍCÍCH EROZNÍ ČINNOSTI

Marie Palíková, Aleš Kučera

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(5), 229-234 | DOI: 10.11118/actaun200957050229

Soil erosion is still an issue in forestry and in human water-resources activities connected with landscape management and the protection of surface waters. The methods recently assessing the water erosion include: monitoring of suspended sediments in water, monitoring of dynamics of soil properties, assessing the inclination of soil towards soil erosion, monitoring of effectiveness of erosion control measures, erosion processes modeling etc. (Buzek, 1981; Buzek, 1983; Holý, 1994; Jařabáč, Belský, 2008).
The river basin of the water tank Šance is very important as a source of potable water and this importance is advanced, when water tank is clogged up by suspended sediments.
Erosion was assessed by two methods in ArcMap 9.3 working with original data: the first method is Universal equation calculating an average annual soil loss from surface (USLE) (Wischmeier, Smith, cit. in Janeček, 2002); the second method assesses the potential erosion (MPE), using specific soil properties as factors, evaluating the rate of the intensity of erosion (Kučera, Palíková; 2009).
Each method uses different ways for the description of the erodibility: USLE describes a long-term average annual soil loss as a consequence of surface erosion. It gives exact values of sediments in t.ha-1.year-1, but from the other point of view, this method is primarily created for an agricultural land. Compared with USLE, MPE solves potential erosion and gives relative values of the erosion tendency of an environment. PME could give a new point of view on the assessing of the erosion.
The river basin Ostravice above water tank Šance was used to compare these two methods. As a control measure, dates of the assessment of the water sediments regime (Buzek, 2001) were used. This observation was pursued in waters of the gagin station ČHMÚ Ostrava in Staré Hamry in according Stehlík (1969). This 25-year process of measuring shows the value of 2.47 t.ha-1.year-1 in water tank upper Ostravice (with surface 72.96 km2). USLE shows much lower values of suspended sediments (0.41 t.ha-1.year-1 using a continuous longitude of slopes or 0.11 t.ha-1.year-1, with regards to the interruption of slopes by the forest roads). This difference is caused by the construction of USLE, which doesn't respect increased values of sediments after the crossing of fords by the forestry mechanization, rill erosion or inputs of sediments from watercourse and near ravines. Therefore, the high values of suspended sediments are mainly caused by forest management (Buzek, 2001).

RECIPROČNÍ DEFORMACE SOUBORŮ POLYURETANOVÝCH PĚN V KONSTRUKCI ČALOUNĚNÉHO NÁBYTKU

Věra Jančová

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(1), 61-70 | DOI: 10.11118/actaun200957010061

The quality of upholstered furniture is currently evaluated through a set of attributes that reflect the "comfort" of the product. Measurable features are evaluated using the test methods, which include an assessment of the product as a whole or only of the individual materials. Methodology to assess materials in the construction of material compositions has not yet been processed before. This work deals with the issue of a evaluation of the material sets of polyurethane foams and their mutual influence. New methodology has been proposed for evaluating mutual deformation of foams sets that has been named "reciprocal deformation of foam sets" in this work. Using this newly proposed method, selected types of polyurethane foams were assessed, in combination with recycled bonding polyurethane foam-type RE 80. The results of the measurements were evaluated and assembled into the graph in order to create a simple tool for the construction of material composition in the mold layer of upholstered furniture. The graph can easily be deducted; it answers the question of what value of deformation is to be considered in drawing up a set of foam materials and recycled PU foam in the construction of material composition.

VLIV PARAZITOCENÓZ NA ZDRAVOTNÍ STAV POPULACE JELENA SIKY (Cervus nippon) V ZÁPADNÍCH ČECHÁCH

Marie Borkovcová, Jan Dvořák, Tomáš Martin

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(5), 33-40 | DOI: 10.11118/actaun200957050033

The aim of this study was detection of prevalence of the parasitecoenoses of sika deer (Cervus nippon) living in the wild in the area of Western Bohemia (Czech Republic) and to compare roundup data with results from previous investigations.
All together 560 samples of excrements and 8 grallochs were examined. The research was done from March 2005 to June 2007. The samples of excrements were collected during the whole research period in periodical monthly intervals always in the amount of 20 pieces. Grallochs were examined by partial helmintological dissection, the samples of excrements by Baermann method and flotation. Parasites were detected only in 26.8 % of examined samples. The spectrum of detected parasites included: coccidia Eimeria spp. (8.6 %), lungworms (LW) Dictyocaulus sp. and Bicaulus sagittatus (23.4 %), and nematode worms from the group of gastrointestinal nematodes (GIN) (7.9 %). In fine we can confirm that the Sika game is quite resistant against parasitoses under our conditions, and health of Sika game in monitored localities is good.

ALTERNATIVNÍ PŘÍSTUP K IDENTIFIKACI BODŮ ZLOMŮ RŮSTOVÉHO POJETÍ HOSPODÁŘSKÉHO CYKLU ČR

Jitka Poměnková

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(3), 123-132 | DOI: 10.11118/actaun200957030123

The purpose of the paper is to identify GDP growth cycle of the Czech Republic by means of turning points identification using alternative statistical method - non-parametric kernel estimate. Special type of estimate, convolution Gasser-Müller one, is used. An advantage of this approach is possibility to estimate derivations of unknown function, which is suitable especially in the case of turning points searching.
For identification of growth business cycle type results of nonparametric estimate of regression function is used. Obtained residuals, growth component, are considered as growth cycle type and are analogically identified. On the basis of nonparametric estimates of growth component derivations (1st and 2nd) turning points (trough and peak) are identified. At the end, comparison of nonparametric statistical approach with economic turning points identification approach, Canova type, is done.

Odezva bramborových hlíz na rázové zatěžování

Šárka Nedomová

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(4), 63-72 | DOI: 10.11118/actaun200957040063

The response of tubers of nine varieties of potatoes to the bar impact have been evaluated. The proposed method enables to obtain force - time record. The response function is represented by the time history of the surface displacement. This function has been recorded using laser vibrometer technique. The main features of the force and displacement function have been found both in the time and frequency domain. It has been found that given method can be used for the detection of the potato tubers damage origin as well as for the differentiation among different varieties of the potatoes. There is also a chance to evaluate the main mechanical characteristics of the potato tubers by non - destructive way.

VLIV POČTU BAKTERIÍ MLÉČNÉHO KVAŠENÍ, PROBIOTICKÝCH KULTUR A HODNOTY PH V KYSANÝCH MLÉČNÝCH VÝROBCÍCH NA JEJICH SENZORICKÉ HODNOCENÍ

Hana Šulcerová, Radka Burdychová

Acta Univ. Agric. Silvic. Mendelianae Brun. 2009, 57(5), 291-304 | DOI: 10.11118/actaun200957050291

Nowadays, we can see on market mainly fermented milk products with addition of probiotic microorganisms, especially strains of Lactobacillus and Bifidobacterium. We can meet also other types of probiotic products. It is recommended to consume at least 100 grams of fermented milk products with minimal concentration of 106 of probiotics in one gram or mililitr of product daily for reaching positive effect on men's health. During fermentation of the carbohydrates, proteins and lipids are disunite and many of aromatic compounds ane compose. They give a typical sensory characteristic to fermented milk products. For quality and quantity level of probiotics, changes of pH value and sensory quality of five kinds of fermented milk product Yoghurt Drink with different flavour were analyzed during the whole expiration period (28 days). Obtained results were statistically evaluated via the analysis of variance and the method of multiple comparison according to Tukey test (P < 0,010) and (P < 0,001). During the minimal endurance time lactic acid bacteria and Bifidobacterium sp. were evaluated and changes of descriptors and pH value were detected. Number of LAB was up to 107 CFU/ml in all samples during 28 days of analysis. Only at sample 2 the number of LAB was 106 CFU/ml. Bifidobacterium sp. grew about degree. The number of LAB and Bifidobacterium sp. of yoghurt drink correspond with public notice number 77/2003 Sb, LAB 107 nad Bifidobacterium sp. 106 KTJ/ml. During 28 days of storage the pH value decreased. The biggest pH drop was recorded between 21. and 28. days of storage in all samples. The beginning pH value was 4.03-4.07 and the final value was between 3.80-3.95. The results of sensory evaluation processed by analysis of dispersion according to type were statistically conlusive in descriptors thickness, texture, intensity of smell, pleasantness of taste and general impression. The results of sensory evaluation processed by analysis of dispersion according to days of storage were statistically conlusive in descriptor pleasantness of smell.

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