Acta Univ. Agric. Silvic. Mendelianae Brun. 2022, 70(1), 31-36 | DOI: 10.11118/actaun.2022.003

Quantification of 'Candidatus Phytoplasma prunorum' in apricot trees exhibiting uneven European stone fruit yellows symptoms

Tomáš Kiss1, Tomáš Nečas1
1 Department of Fruit Science, Faculty of Horticulture in Lednice, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic

This study focused on quantification of 'Candidatus Phytoplasma prunorum' in apricot trees exhibiting uneven symptoms of European stone fruit yellows. For the research, 17 trees showing early leaf bud break in one part of the crown were selected in winter season, and 13 trees showing leafroll in older part of the shoots and no symptoms in younger part of the same shoots were selected in summer season. Absolute quantification of phytoplasmas was performed by real-time PCR in samples collected from asymptomatic and symptomatic parts. The results showed, that phytoplasma could not be always detected in asymptomatic parts, where 2 out of 17 samples from asymptomatic parts from winter season were negative while in summer season 6 out of 13 samples from asymptomatic parts were negative. Comparison of phytoplasma quantities between symptomatic and asymptomatic plant parts then showed significantly higher quantities in symptomatic parts from samples collected in summer season and no quantity difference in samples collected in winter season.

Keywords: real-time PCR, 'Ca. P. prunorum', early leaf bud break, leafroll, ESFY, Prunus armeniaca

Received: October 25, 2021; Revised: January 7, 2022; Accepted: January 25, 2022; Published: March 1, 2022  Show citation

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Kiss, T., & Nečas, T. (2022). Quantification of 'Candidatus Phytoplasma prunorum' in apricot trees exhibiting uneven European stone fruit yellows symptoms. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 70(1), 31-36. doi: 10.11118/actaun.2022.003
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