Acta Univ. Agric. Silvic. Mendelianae Brun. 2021, 69(5), 569-578 | DOI: 10.11118/actaun.2021.051

Biocidal Activity of Origanum vulgare subsp. hirtum Essential Oil

Milena Nikolova1, Mariya Yovkova1, Elina Yankova-Tsvetkova1, Boryanka Traikova1, Tatyana Stefanova1, Ina Aneva1, Strahil Berkov1
1 Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria

Toxic effect of Origanum vulgare subsp. hirtum (Link) Ietsw. essential oil was assayed on weeds (Lolium perenne L. Trifolium pratense L. and Trifolium repens L.), potato plants (Solanum tuberosum L.) and Myzus persicae Sulz. The GC-MS analysis of the essential oil revealed carvacrol as the main component. The essential oil solutions completely inhibited the seed germination of the target weeds at concentration 2 μL/mL in Petri dishes. In contrast, in soil, this effect was achieved at a concentration of 5 µL/mL for studied dicot and at 10 µL/mL for the monocot species. Total destruction of target weeds at post-emergence stage after spraying with solution of the essential oil in concentrate 10 µL/ mL was found. Mature potato plants were poorly affected by the essential oil. A completely toxic effect on M. persicae was established by applying a solution with a concentration of 3 μL/mL of the essential oil. The experimental data indicate that the essential oil of the species has great potential, as an alternative to synthetic biocides, for the production of eco-friendly formulations for pest control.

Keywords: phytotoxicity, insecticidal, carvacrol, Greek oregano, Myzus persicae, weeds

Received: September 7, 2020; Revised: June 17, 2021; Accepted: September 17, 2021; Published: November 1, 2021  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Nikolova, M., Yovkova, M., Yankova-Tsvetkova, E., Traikova, B., Stefanova, T., Aneva, I., & Berkov, S. (2021). Biocidal Activity of Origanum vulgare subsp. hirtum Essential Oil. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 69(5), 569-578. doi: 10.11118/actaun.2021.051
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. BLANCO, C., GIRALDEZ, F. J., PRIETO, N., MORAN, L., ANDRES, S., BENAVIDES, J., TEJIDO, M. L. and BODAS, R. 2014. Effects of dietary inclusion of sunflower soap stocks on nutrient digestibility, growth performance, and ruminal and blood metabolites of light fattening lambs. Journal of Animal Science, 92(9): 4086-4094. Go to original source...
    2. ABBOTT, W. S. 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18(2): 265-267. Go to original source...
    3. ADAM, K., SIVROPOULOU, A., KOKKINI, S., LANARAS, T. and ARSENAKIS, M. 1998. Antifungal activities of Origanum vulgare subsp. hirtum, Mentha spicata, Lavandula angustifolia, and Salvia fruticosa essential oils against human pathogenic fungi. Journal of Agricultural and Food Chemistry, 46(5): 1739-1745. Go to original source...
    4. ADAMS, R. 2007. Identification of essential oil components by gas chromatography/mass spectrometry. 4th Edition. Allured Pub Corp.
    5. AMRI, I., HAMROUNI, L., HANANA, M. and JAMOUSSI, B. 2012. Herbicidal potential of essential oils from three Mediterranean trees on different weeds. Current Bioactive Compounds, 8(1): 3-12. Go to original source...
    6. AMRI, I., HAMROUNI, L., HANANA, M. and JAMOUSSI, B. 2013a. Reviews on phytotoxic effects of essential oils and their individual components: news approach for weeds management. International Journal of Applied Biology and Pharmaceutical Technology, 4: 96-114.
    7. AMRI, I., HAMROUNI, L., HANANA, M., GARGOURI, S., FEZZANI, T. and JAMOUSSI, B. 2013b. Chemical composition, physico-chemical properties, antifungal and herbicidal activities of Pinus halepensis Miller essential oils. Biological Agriculture and Horticulture, 29(2): 91-106. Go to original source...
    8. AMRI, I., HANANA, M., JAMOUSSI, B. and JAMOUSSI, B. 2017. Essential oils of Pinus nigra J. F. Arnold subsp. laricio Maire: Chemical composition and study of their herbicidal potential. Arabian Journal of Chemistry, 10(S2): S3877-S3882. Go to original source...
    9. FAO. 1979. Recommended methods for the detection and measurement of resistance of agricultural pests to pesticides, Method for adult aphids. FAO Plant Protection Bulletin, 27: 29-32.
    10. ARANITI, F., LANDI, M., LUPINI, A., SUNSERI, F., GUIDI, L. and ABENAVOLI, M. 2018. Origanum vulgare essential oils inhibit glutamate and aspartate metabolism altering the photorespiratory pathway in Arabidopsis thaliana seedlings. Journal of Plant Physiology, 231: 297-309. Go to original source...
    11. ATAKM, M., MAVI, K. and UREMIS, I. 2016. Bio-herbicidal effects of oregano and rosemary essential oils on germination and seedling growth of bread wheat cultivars and weeds. Romanian Biotechnological Letters, 21(1): 11149-11159.
    12. ATANASOVA, D., GANCHEV, D. and NENOV, N. 2018. Efficacy of some plant essential oils against cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae) under laboratory conditions. Journal of Agricultural Science, 1: 10-16.
    13. BASER, K. H. C., ÖZEK, T., KÜRKCÜOGLU, M. and TÜMEN, G. 1994. The essential oil of Origanum vulgare subsp. hirtum of Turkish origin. Journal of Essential Oil Research, 6(1): 31-36. Go to original source...
    14. BENDRE, R. S., BAGUL, S. D. and RAJPUT, J. 2018. Carvacrol: an excellent natural pest control agent. Natural Products Chemistry and Research, 6(6): 1000349.
    15. BENVENUTI, S., CIONI P. L., FLAMINI, G. and PARDOSSI, A. 2017. Weeds for weed control: Asteraceae essential oils as natural herbicides. Weed Research, 57(5): 342-353. Go to original source...
    16. BERKOV, S., MUTAFOVA, B. and CHRISTEN, P. 2014. Molecular biodiversity and recent analytical developments: a marriage of convenience. Biotechnology Advances, 32(6): 1102-1110. Go to original source...
    17. BLACKMAN, R. L. and EASTOP, V. F. 2000. Aphids on The World's Crops. An identification and Information Guide. 2nd Edition. London: The Natural History Museum.
    18. BLÁZQUEZ, M. A. and CARBÓ, E. 2015. Control of Portulaca oleracea by boldo and lemon essential oils in different soils. Industrial Crops and Products, 76: 515-521. Go to original source...
    19. DANCEWICZ, K., KARDAN, B., SZUMNY, A. and GABRYS, B. 2012. Aphid behaviour-modifying activity of essential oils from Lamiaceae and Apiaceae. Aphids and Other Hemipterous Insects, 18: 93-100.
    20. DAYAN, F. E., CANTRELL, C. L. and DUKE, S. O. 2009. Natural products in crop protection. Bioorganic and Medicinal Chemistry, 17(12): 4022-4034. Go to original source...
    21. DHIFI, W., BELLINI, S., JAZI, N., BAHLOUL, S. and MNIF, W. 2016. Essential oils' chemical characterization and investigation of some biological activities: a critical review. Medicines, 3(4): 25. Go to original source...
    22. DE ALMEIDA, L. F. R., FREI, F. MANCINI, E., DE MARTINO, L. and DE FEO, V. 2010. Phytotoxic activities of Mediterranean essential oils. Molecules, 15(6): 4309-4323. Go to original source...
    23. DE MARTINO, L., DE FEO V., FORMISANO, C., MIGNOLA, E. and SENATORE, F. 2009. Chemical composition and antimicrobial activity of the essential oils from three chemotypes of Origanum vulgare L. ssp. hirtum (Link) Ietswaart growing wild in Campania (Southern Italy). Molecules, 14(8): 2735-2746. Go to original source...
    24. DIGILIO, M. C., MANCINI E., VOTO, E. and DE FEO, V. 2008. Insecticide activity of Mediterranean essential oils. J Plant Interact., 3(1): 17-23. Go to original source...
    25. DRAGOEVA, A. P., KOLEVA, V., NANOVA, Z. D., KASCHIEVA, M. Z. and YOTOVA, I. R. 2014. Allelopathic and cytotoxic activity of Origanum vulgare ssp. vulgare growing wild in Bulgaria. Chemistry: Bulgarian Journal of Science Education, 23(6): 914-924.
    26. DUBEY, N., SRIVASTAVA, B. and KUMAR, A. 2008. Current status of plant products as botanical pesticides in storage pest management. Journal of Biopesticides, 1: 182-186.
    27. GARCÍA-BELTRÁN, J. M. and ESTEBAN, M. A. 2016. Properties and applications of plants of Origanum sp. genus. SM Journal of Biology, 2(1): 1006.
    28. GONZALEZ-COLOMA, A., REINA, M., DIAZ, C., FRAGA M. and SANTANA-MERIDAS, O. 2013. Natural product-based biopesticides for insect control. In: Reference Module in Chemistry, Molecular Sciences and Chemical Engineering. Elsevier. Go to original source...
    29. GRONDONA, E., GATTI, G., LÓPEZ, A. G., SÁNCHEZ, L. R., RIVERO, V., PESSAH, O., ZUNINO, M. P. and PONCE, A. A. 2014. Bio-efficacy of the essential oil of Oregano (Origanum vulgare Lamiaceae. Ssp. hirtum). Plant Foods for Human Nutrition, 69(4): 351-357. Go to original source...
    30. GRULOVÁ, D., CAPUTO L., ELSHAFIE, H. S., BARANOVÁ, B., DE MARTINO, L., SEDLÁK, V., GOGALOVÁ, Z., PORÁCOVÁ, J., CAMELE, I. and DE FEO, V. 2020. Thymol chemotype Origanum vulgare L. essential oil as a potential selective bio-based herbicide on monocot plant species. Molecules, 25(3): 595. Go to original source...
    31. FRABBONI, L., TARANTINO, A., PETRUZZI, F. and DISCIGLIO, G. 2019. Bio-herbicidal effects of oregano and rosemary essential oils on chamomile (Matricaria chamomilla L.) crop in organic farming system. Agronomy, 9(9): 475. Go to original source...
    32. HAZRATI, H., SAHARKHIZ, M. J., NIAKOUSARI, M. and MOEIN, M. 2017. Natural herbicide activity of Satureja hortensis L. essential oil nanoemulsion on the seed germination and morphophysiological features of two important weed species. Ecotoxicology and Environmental Safety, 142: 423-430. Go to original source...
    33. IBÁÑEZ, M. D. and BlÁZQUEZ, M. A. 2018. Phytotoxicity of essential oils on selected weeds: potential hazard on food crops. Plants, 7(4): 79. Go to original source...
    34. IBÁÑEZ, M. D. and BlÁZQUEZ, M. A. 2017. Herbicidal value of essential oils from oregano-like flavour species. Food and Agricultural Immunology, 28(6): 1168-1180. Go to original source...
    35. KAUR, S., SINGH, H. P., MITTAL, S., BATISH, D. R. and KOHLI, R. K. 2010. Phytotoxic effects of volatile oil from Artemisia scoparia against weeds and its possible use as a bioherbicide. Industrial Crops and Products, 32(1): 54-61. Go to original source...
    36. KONAKCHIEV, A., GENOVA, E. and COULADIS, M. 2004. Chemical composition of the essential oil of Origanum vulgare ssp. hirtum (Link) Ietswaart in Bulgaria. Comptes rendus de l'Academie bulgare des Sciences, 57: 49-52.
    37. LA PERGOLA, A., RESTUCCIA, C., NAPOLI, E., BELLA, S., BRIGHINA, S., RUSSO A. and SUMA, P. 2017. Commercial and wild Sicilian Origanum vulgare essential oils: chemical composition, antimicrobial activity and repellent effects. Journal of Essential Oil Research, 29(6): 451-460. Go to original source...
    38. LI, X., BERENBAUM, M. R. and SCHULER, M. A. 2000. Molecular cloning and expression of CYP6B8: a xanthotoxin inducible cytochrome P450 cDNA from Helicoverpa zea. Insect Biochemistry and Molecular Biology, 30(1): 75-84. Go to original source...
    39. MANN, R. S. and KAUFMAN, E. 2012. Natural product pesticides: Their development, delivery and use against insect vectors. Mini-Reviews in Organic Chemistry, 9(2): 185-202. Go to original source...
    40. MANCINI, E., CAMELE, I., ELSHAFIE, H. S., DE MARTINO, L., PELLEGRINO, C., GRULOVA, D. and DE FEO, V. 2014. Chemical composition and biological activity of the essential oil of Origanum vulgare ssp. hirtum from different areas in the southern Apennines (Italy). Chemistry and Biodiversity, 11(4): 639-651. Go to original source...
    41. PETROVIĆ-OBRADOVIĆ, O., LECLANT, F. and TOMANOVIĆ, ®. 2011. Catalogue of Aphids of Durmitor (Insecta, Hemiptera, Aphididae). Podgorica: Montenegrin Academy of Sciences and Arts.
    42. SARIKURKCU, C., ZENGIN, G., OSKAY, M., UYSAL, S., CEYLAN, R. and AKTUMSEK, A. 2015. Composition, antioxidant, antimicrobial and enzyme inhibition activities of two Origanum vulgare subspecies (subsp. vulgare and subsp. hirtum) essential oils. Industrial Crops and Products, 70: 178-184. Go to original source...
    43. SCHILLACI, D., NAPOLI, E. M. CUSIMANO, M. G., VITALE, M. and RUBERTO, G. 2013. Origanum vulgare subsp. hirtum essential oil prevented biofilm formation and showed antibacterial activity against planktonic and sessile bacterial cells. Journal of Food Protection, 76(10): 1747-1752. Go to original source...
    44. SCOGNAMIGLIO, M., D'ABROSCA, B., ESPOSITO, A., PACIFICO, S., MONACO, P. and FIORENTINO, A. 2013. Plant growth inhibitors: allelopathic role or phytotoxic effects? Focus on Mediterranean biomes. Phytochemistry Reviews, 12(4): 803-830. Go to original source...
    45. SYNOWIEC, A., KALEMBA, D., DROZDEK, E. and BOCIANOWSKI, J. 2017. Phytotoxic potential of essential oils from temperate climate plants against the germination of selected weeds and crops. Journal of Pest Science, 90(1): 407-419. Go to original source...
    46. TRAYKOVA, B., STANILOVA, M., NIKOLOVA, M. and BERKOV, S. 2019. Growth and essential oils of Salvia officinalis plants derived from conventional or aeroponic produced seedlings. Agriculturae Conspectus Scientificus, 84(1): 77-81.
    47. TWORKOSKI, T. 2002. Herbicide effects of essential oils. Weed Science, 50(4): 425-431. Go to original source...
    48. ULUKANLI, Z., CENET, M., HUSEYIN, I. and YILMAZTEKIN, M. 2018. Antimicrobial and herbicidal activities of the essential oil from the Mediterranean Thymus eigii. Journal of Essential Oil Bearing Plants, 21(1): 214-222. Go to original source...
    49. VASILAKOGLOU, I., DHIMA, K., ANASTASSOPOULOS, E., LITHOURGIDIS, A., GOUGOULIAS N. and CHOULIARAS, N. 2011. Oregano green manure for weed suppression in sustainable cotton and corn fields. Weed Biology and Management, 11(1): 38-48. Go to original source...
    50. VERES, K., VARGA, E., DOBOS, A., HAJDÚ, Z. S., MÁTHÉ, I., NÉMETH, E. and SZABÓ, K. 2003. Investigation of the composition and stability of the essential oils of Origanum vulgare ssp. vulgare L. and O. vulgare ssp. hirtum (Link) Ietswaart. Chromatographia, 57(1-2): 95-98. Go to original source...
    51. VOKOU, D., DOUVLI, P., BLIONIS, G. J. and HALLEY, J. M. 2003. Effects of monoterpenoids, acting alone or in pairs, on seed germination and subsequent seedling growth. Journal of Chemical Ecology, 29: 2281-2301. Go to original source...
    52. YANKOVA-TSVETKOVA, E., NIKOLOVA, M., ANEVA, I., STEFANOVA, T. and BERKOV, S. 2020. Germination inhibition bioassay of extracts and essential oils from plant species. Comptes rendus de l'Académie bulgare des Sciences, 73(9): 1254-1259. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY NC ND 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content