Acta Univ. Agric. Silvic. Mendelianae Brun. 2021, 69(1), 91-100 | DOI: 10.11118/actaun.2021.010

Effect of Increasing Zearalenone Levels on the Technologically Problematic Microorganisms and Food Risky Pathogens (in Vitro)

Ludmila Křížová1, Marcela Klimešová2, Oto Hanuš2, Irena Němečková2, Petr Roubal2, Jana Tšponová1, Miroslav Skřivánek2, Ludmila Nejeschlebová2, Radoslava Jedelská2
1 Department of Animal Breeding, Animal Nutrition and Biochemistry, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1946/1, 612 42 Brno, Czech Republic
2 Dairy Research Institute Ltd., Ke Dvoru 12a, 160 00 Prague, Czech Republic

The aim of this study was to determine the effect of different zearalenone (ZEA) concentrations (0, 10, 100, 250, 500, 1 000 µg/l) on growth of Bacillus cereus, Staphylococcus aureus and Pseudomonas fluorescens in milk. The samples were incubated for 6 days at 22 and 30 °C (B. cereus and S. aureus) or at 6.5 and 22 ℃ (P. fluorescens), respectively. Counts of bacteria in milk were measured every 24 hours. Maximum counts of B. cereus after 144 h of incubation at 30 ℃ ranged from 7.00 to 7.78 log CFU/ml. The most significant effect of ZEA on B. cereus across the experiment was observed after 96 h of incubation at 22 °C and after 72 h at 30 °C (P < 0.001 and P < 0.01, respectively). S. aureus maintained at 30 °C showed similar growth parameters as at 22 ℃ regardless of ZEA presence. The most significant effects of ZEA on S. aureus were after 120 h of incubation at 22 °C and after 72 h at 30 °C (P < 0.001). The growth of P. fluorescence at 6.5 °C was slower compared to growth at 22 °C. The most significant effect of ZEA between ZEA spiked and Z0 samples on P. fluorescence across the experiment was observed after 48 hours of incubation at 22 °C (P < 0.001).

Keywords: mycotoxin, milk, viability, Bacillus cereus, Staphylococcus aureus, Pseudomonas fluorescens

Received: September 15, 2020; Accepted: January 5, 2021; Published: March 1, 2021  Show citation

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Křížová, L., Klimešová, M., Hanuš, O., Němečková, I., Roubal, P., Tšponová, J., ... Jedelská, R. (2021). Effect of Increasing Zearalenone Levels on the Technologically Problematic Microorganisms and Food Risky Pathogens (in Vitro). Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 69(1), 91-100. doi: 10.11118/actaun.2021.010
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    36. Ludmila Křížová: krizoval@vfu.czMarcela Klimešová: marcela.vyletelova@seznam.czOto Hanuš: hanus.oto@seznam.czIrena Němečková: nemeckova@milcom-as.czPetr Roubal: roubal@milcom-as.czJana Tšponová: tsponovaj@vfu.czMiroslav Skřivánek: farmavs@seznam.czLudmila Nejeschlebová: ludmila.mnejeschlebova@seznam.czRadoslava Jedelská: Radka.Jedelska@seznam.cz

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