Acta Univ. Agric. Silvic. Mendelianae Brun. 2020, 68(3), 491-496 | DOI: 10.11118/actaun202068030491

Effect of Nitrogen Fixing Bacteria and Nitrogen Rate on Yield and Growth of Common Bean

Somaye Fallahi, Peyman Sharifi
Department of Agronomy and Plant Breeding, Rasht Branch, Islamic Azad University, Rasht, Iran

To study the effects of nitrogen (N) rate and co-inoculation of nitrogen-fixing bacteria (Rhizobium phaseoli) on common bean, an experiment was performed in Rasht, Iran. Common bean genotype, Guilanian landrace, was planted in a factorial experiment based on a randomized complete block design with three replicates on April, 15th in 2014. The first and second factors were nitrogen rates (0, 30, 60, 90 and 120 kg.ha-1 as urea) and Rhizobium phaseoli (inoculation and un-inoculation), respectively. Nitrogen had significant effect on seed yield and plant height. The effect of Rhizobium phaseoli was significant on seed yield, plant height, pod length, pods per plant, seeds per pod and nitrogen content in leaf. Interaction effects of nitrogen and Rhizobium phaseoli were not significant on any of the traits. Application of 60 kg.ha-1 nitrogen significantly improved seed yield, plant height and number of pods per plant. Nitrogen supply beyond the 60 kg.ha-1 decreased plant height, pods per plant and seed yield. The greatest values of seed yield (1635 kg.ha-1), plant height (30.01 cm), pod length (13.19 cm), number of pods per plant (6.7), number of seeds per pod (4.34) and nitrogen content in leaf (4.12%) was observed in inoculation treatment. The results indicated the inoculation of common bean seeds by Rhizobium phaseoli improved the plant growth, productivity and quality. Therefore, the common bean inoculation with Rhizobium phaseoli is suitable to achieve the yield potential and reduce the adverse effects of environmental and may be recommended due to its advantages in terms of reduced application of nitrogen fertilizer.

Keywords: Phaseolus vuigaris, protected agriculture, Rhizobium phaseoli, mineral, yield

Received: March 4, 2019; Accepted: June 8, 2020; Published: July 1, 2020  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Fallahi, S., & Sharifi, P. (2020). Effect of Nitrogen Fixing Bacteria and Nitrogen Rate on Yield and Growth of Common Bean. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 68(3), 491-496. doi: 10.11118/actaun202068030491
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. ABDZAD GOHARI, A. and AMIRI, E. 2010. Increase of bean production in iron and nitrogen fertilization in sustainable agriculture. In: First National Congress of Sustainable Agriculture and Health Crop Production. Isfahan, pp. 230-236.
    2. AHMADI-RAD, S., GHOLAMHOSEINI, M., GHALAVAND, A., ASGHARZADEH, A. and DOLATABADIAN, A. 2016. Foliar application of nitrogen fixing bacteria increases growth and yield of canola grown under different nitrogen regimes. Rhizosphere, 2: 34-37. DOI: 10.1016/j.rhisph.2016.08.006 Go to original source...
    3. ALIKHANI, H. A., SALEH-RASTIN, N. and ANTOUM, H. 2006. Phosphate solubilizing activity of rhizobia native to Iranian soils. Plant and Soil, 287: 35-41. DOI: 10.1007/s11104-006-9059-6 Go to original source...
    4. ASLAM, M., AHMAD, H. K., TULLAH, H., AYAZ, M., AHMAD, E., SAGOO, A. G., ULLAH, I., HUSSAIN, A. and MANZOOR. M. 2010. Nodulation, grain yield and grain protein contents as affected by rhizobium inoculation and fertilizer placement in chickpea cultivar bittle-98. Sarhad Journal of Agriculture, 26(4): 467-474.
    5. BACEM, M., AOUANI, M. E. and MHAMDI, R. 2007. Nodulation and growth of common bean (Phaseolus vulgaris) under water deficiency. Soil Biology and Biochemistry, 39(7): 1744-1750. Go to original source...
    6. BOZORGI, H. R., AZARPOUR, E. and MORADI, M. 2011. The effects of bio, mineral nitrogen fertilization and foliar Zinc spraying on yield and yield components of faba bean. World Applied Science Journal, 13(6): 1409-1414.
    7. DIXON, R. and KAHN, D. 2004. Genetic regulation of biological nitrogen fixation. Nature Reviews Microbiology, 2: 621-631. DOI: 10.1038/nrmicro954 Go to original source...
    8. EVANS, J., MCNEIL, A. M., UNKOVICH, M. J., FETTELL, N. A. and HEENAN. D. P. 2001. Net nitrogen balances for cool-season grain legume crops and contributions to wheat nitrogen uptake: a review. Australian Journal of Experimental Agriculture, 41(3): 347-359. DOI: 10.1071/EA00036 Go to original source...
    9. FIGUEIREDO, M. V. B., MARTÍNEZ, C. R., BURITY, H. A. and CHANWAY. C. P. 2007. Plant growth-promoting rhizobacteria for improving nodulation and nitrogen fixation in the common bean (Phaseolus vulgaris L.). World Journal of Microbiology and Biotechnology, 24: 1187-1193. DOI: 10.1007/s11274-007-9591-4 Go to original source...
    10. GEETHA, V. and VARUGHESE, K. 2001. Response of vegetable cowpea to nitrogen and potassium under varying methods of irrigation. Journal of Tropical Agriculture, 39: 111-113.
    11. GICHARU, G., GITONGA, N., BOGA, H., CHERUIYOT, R. and MAINGI, J. 2013. Effect of inoculating selected climbing bean cultivars with different rhizobia strains on nitrogen fixation. Online International Journal of Microbiological Research, 1: 25-31.
    12. HABETE, A. and BURAKA, T. 2016. Effect of Rhizobium inoculation and nitrogen fertilization on nodulation and yield response of common bean (Phaseolus vulgaries L.) at Boloso Sore, Southern Ethiopia. Journal of Biological, Agriculture and Healt, 6(13): 72-75.
    13. JOHN NDLOVU, T. 2015. Effect of rhizobium phaseoli inoculation and phosphorus application on nodulation, growth and yield components of two drybean (Phaseolus vulgaris L.) cultivars. Master Thesis. Faculty of Science and Agriculture (School of Agricultural and Environmental Sciences) at the University of Limpopo, South Africa.
    14. KJELDAHL, J. Z. 1983. A new method for the determination of nitrogen in organic bodies. Anals of Chemistry, 22: 366-382. DOI: 10.1007/BF01338151 Go to original source...
    15. LAMPTEY, S., AHLABOR, B. D. K., YEBOAH, S. and OSEI, D. 2014. Effect of Rhizobium inoculants and reproductive growth stages on shoot biomass and yield of soybean (Glycine max (L.) Merril.). Journal of Agricultural Science, 6: 44-54. DOI: 10.5539/jas.v6n5p44 Go to original source...
    16. MARTINS, A., KIMURA, O., RIBEIRO, R. L. D. and BALDANI, J. I. 2003. Efeito da microbiolização de sementes com rizobactérias fluorescentes do gênero Pseudomonas so- bre a "murcha fusariana" do feijoeiro (Phaseolus vulgaris L.). Agronomy Journal, 37(1): 69-75.
    17. OLIVEIRA, I. P., ARAÚJO, R. S. and DUTRA, L. G. 1996. Nutriçâo mineral e fixaçâo biológica do nitrogênio. In: ARAUJO, R. S. et al. (Eds.). Cultura do feijoeiro comum no Brasil. Piracicaba Potafos, pp. 169-221.
    18. OTIENO, P. E. and MUTHOMI, J. W. G. N. 2009. Cheminingwa, and Nderitu J. W. Effect of rhizobia inoculation, farmyard manure and nitrogen fertilizer on growth, nodulation and yield of selected food legumes. Journal of Bilogical Science, 9(4): 326-332. Go to original source...
    19. OUFDOU, K., BECHTAOUI, N., EL ALAOUI, A., BENIDIRE, L., DAOUI, K. and GÖTTFERT, M. 2016. Symbiotic Rhizobacteria for improving of the agronomic effectiveness of phosphate fertilizers. Proceeding and Engineering, 138: 325-331. DOI: 10.1016/j.proeng.2016.02.092 Go to original source...
    20. RONNER, E., FRANKE, A. C., VANLAUWEC, B., DIANDAD, M., EDEHE, E., UKEME, B., BALAF, A., HEERWAARDENA, J. and GILLER, K. E. 2016. Understanding variability in soybean yield and response to P-fertilizerand rhizobium inoculants on farmers' fields in northern Nigeria. Field Crops Reserch, 186: 133-145. DOI: 10.1016/j.fcr.2015.10.023 Go to original source...
    21. SALEHIN, F. and RAHMAN, S. 2012. Effects of zinc and nitrogen fertilizer and their application method on yield and yield components of Phaseolus vulgaris L. Agricultural Sciences, 3(1): 9-13. DOI: 10.4236/as.2012.31003 Go to original source...
    22. SOARES, A. L. L., PEREIRA, J. P. A. R., FERREIRA, P. A. A., VALE, H. M. M., LIMA, A. S., ANDRADE, M. J. B. and MOREIRA, F. M. S. 2006. Agronomic efficiency of selected rhizobia strains and diversity of native nodulating populations in Perdões (MG-Brazil). I, Cowpea. Revista Brasileira de Ciência do Solo, 30: 795-802. DOI: 10.1590/S0100-06832006000500005 Go to original source...
    23. SORATTO, R. P., PEREZ, A. A. G. and MANZATTO, N. P. 2010. Nitrogen management for common bean crop in new and established no-tillage systems. In: 19th World Congress of Soil Science, Soil Solutions for a Changing World. 1-6 August, Brisbane, Australia.
    24. STAJKOVIC, O., DELIC, D., JOSIC, D., KUZMANOVIC, D., RASULIC, N. and KNEZEVICVUKCEVIC, J. 2011. Improvement of common bean growth by coinoculation with Rhizobium and plant growth-promoting bacteria. Romanian Biotechnology Letter, 16(1): 5919-5926.
    25. TAIRO, E. V. and NDAKIDEEMI, P. A. 2013. Yields and economic benefits of soybean (Glycine max L.) as affected by Bradyrhizobium japonicum inoculation and phosphorus supplementation. American Journal of Research and Communication, 1(11): 159-172.
    26. UYANÖZ, R. and ÜMMÜHAN, K. 2011. Effects of different salt concentrations and Rhizobium inoculation (native and Rhizobium tropici CIAT899) on growth of dry bean (Phaseolus vulgaris L.). European Journal of Soil and Biology, 47(6): 387-391. DOI: 10.1016/j.ejsobi.2011.07.007 Go to original source...
    27. VARGAS, M. A. T., MENDES, L. C. and HANGRIA. M. 2000. Response of field grown bean to Rhizobium inoculation and nitrogen fertilization in two cerrados soils. Fertility of Soils, 32: 228-233. DOI: 10.1007/s003740000240 Go to original source...
    28. XU, B. J. and CHANG, S. K. C. 2008. Total phenolic content and antioxidant properties of eclipse lack beans (Phaseolus vulgaris L.) as affected by processing methods. Journal of Food Science, 73: 19-27. DOI: 10.1111/j.1750-3841.2007.00625.x Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY NC ND 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content