Acta Univ. Agric. Silvic. Mendelianae Brun. 2019, 67(5), 1171-1182 | DOI: 10.11118/actaun201967051171

Endophytic Bacterial Consortium Originated from Forestry Plant Roots and Their Nematicidal Activity against Meloidogyne incognita Infestation in Greenhouse

Abdul Munif1, Supramana1, Elis Nina Herliyana2, Ankardiansyah Pandu Pradana3
1 Department of Plant Protection, Faculty of Agriculture, IPB University, Jl Kamper, Kampus IPB Darmaga Wing 7 Level 5, Bogor, West Java, 16680, Indonesia
2 Department of Silviculture, Faculty of Forestry, IPB University, Jl Lingkar Akademik, Bogor, West Java, 16680, Indonesia
3 Department of Plant Protection, Faculty of Agriculture, University of Jember, Jl Kalimantan No. 37, Jember, East Java, 68121, Indonesia

Yield loss due to root-knot nematode Meloidogyne incognita infection is reported to reach 35%, depends on factors contributing to infection. Application of several endophytic bacterial isolates (bacterial consortium) to control pathogenic infection is reported to be more effective compared to the application of single bacterial isolate. This study was aimed to obtain endophytic bacterial consortium originated from forestry plant that is effective to control root-knot nematode. The study was conducted through bacterial isolation followed by biosafety test. Bacterial isolates that were found to be safe for plants and mammals and compatible with each other were further grouped as the endophytic bacterial consortium. Phenotypic characterization and physiological characteristics including Gram type, ability to produce protease, chitinase, and lipase enzymes as well as HCN volatile compound were also tested. Moreover, the ability to fix nitrogen and dissolve phosphate were also examined. The endophytic bacterial consortium consisted of several bacterial isolates was further tested for its ability to inhibit M. incognita egg hatching and increase J2 of M. incognita mortality in vitro. Furthermore, test on tomato plants infested with 500 J2 of M. incognita was also performed in the greenhouse. Test results showed that 70 bacterial isolates were successfully isolated from Shorea sp., Swietenia sp., Albizia falcataria, Anthocephalus cadamba, and Juglans nigra. However, 34 bacterial isolates were observed to be safe (did not cause hypersensitivity reaction and did not produce hemolytic toxin). According to physiological characteristics, it was found that 25 isolates were able to produce protease enzyme, 26 isolates were able to produce chitinase enzyme, and 14 isolates were able to produce lipase enzyme. Moreover, it was also detected that 11 isolates were able to produce HCN volatile compound, 23 isolates were able to fix nitrogen (N), and 24 isolates were able to dissolve phosphate (P). Endophytic bacterial consortium obtained in this study was also observed to be able to inhibit M. incognita egg hatching up to 81.33% and increase J2 of M. incognita mortality up to 85% compared to control. In addition, the application of endophytic bacterial consortium was also able to increase the growth of tomato plant infected with M. incognita, and suppress the severity of the root-knot disease. This study provided information that endophytic bacterial consortium originated from forestry plants has the potential as a biocontrol agent of M. incognita.

Keywords: bio-control, bio-stimulant enzyme, filtrate culture, root-knot nematode

Received: April 6, 2019; Accepted: October 7, 2019; Published: October 31, 2019  Show citation

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Munif, A., Supramana,, Herliyana, E.N., & Pradana, A.P. (2019). Endophytic Bacterial Consortium Originated from Forestry Plant Roots and Their Nematicidal Activity against Meloidogyne incognita Infestation in Greenhouse. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 67(5), 1171-1182. doi: 10.11118/actaun201967051171
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    References

    1. ABO-ELYOUSR, K., KHAN, Z. and ABEDEL-MONEIM, M. 2010. Evaluation of plant extracts and Pseudomonas for control of root-knot nematode, Meloidogyne incognita on tomato. Nematropica, 40(2): 289-299.
    2. ASHOUB, A. and AMARA, M. 2010. Biocontrol activity of some bacterial genera against root-knot nematode, Meloidogyne incognita. Journal of American Science, 6(10): 321-328.
    3. BACKMAN, P. A. and SIKORA, R. A. 2008. Endophytes: an emerging tool for biological control. Biological Control, 46(1): 1-3. DOI: 10.1016/j.biocontrol.2008.03.009 Go to original source...
    4. BALDANI, J., BALDANI, V., SELDIN, L. and DÖBEREINER, J. 1986. Characterization of Herbaspirillum seropedicae gen. nov., sp. nov., a root-associated nitrogen-fixing bacterium. International Journal of Systematic and Evolutionary Microbiology, 36(1): 86-93. Go to original source...
    5. BERTRAND, B., NUNEZ, C. and SARAH, J. L. 2000. Disease complex in coffee involving Meloidogyne arabicida and Fusarium oxysporum. Plant Pathology, 49(3): 383-388. DOI: 10.1046/j.1365-3059.2000.00456.x Go to original source...
    6. BHATTI, D. and JAIN, R. K. 1977. Estimation of loss in okra, tomato and brinjal yield due to Meloidogyne incognita. Indian Journal of Nematology, 7(1): 37-41.
    7. CLAUS, D. 1992. A standardized Gram staining procedure. World Journal of Microbiology and Biotechnology, 8(4): 451-452. DOI: 10.1007/BF01198764 Go to original source...
    8. COMPANT, S., DUFFY, B., NOWAK, J., CLÉMENT, C. and BARKA, E. A. 2005. Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects. Applied Environmental Microbiology, 71(9): 4951-4959. DOI: 10.1128/AEM.71.9.4951-4959.2005 Go to original source...
    9. DONG, Z., CANNY, M. J., MCCULLY, M. E., ROBOREDO, M. R., CABADILLA, C. F., ORTEGA, E. and RODES, R. 1994. A nitrogen-fixing endophyte of sugarcane stems (a new role for the apoplast). Plant Physiology, 105(4): 1139-1147. DOI: 10.1104/pp.105.4.1139 Go to original source...
    10. ELBELTAGY, A., NISHIOKA, K., SATO, T., SUZUKI, H., YE, B., HAMADA, T., ISAWA, T., MITSUI, H. and MINAMISAWA, K. 2001. Endophytic colonization and in planta nitrogen fixation by a Herbaspirillum sp. isolated from wild rice species. Applied Environmental Microbiology, 67(11): 5285-5293. DOI: 10.1128/AEM.67.11.5285-5293.2001 Go to original source...
    11. EL-DEEB, B., FAYEZ, K. and GHERBAWY, Y. 2013. Isolation and characterization of endophytic bacteria from Plectranthus tenuiflorus medicinal plant in Saudi Arabia desert and their antimicrobial activities. Journal of Plant Interactions, 8(1): 56-64. DOI: 10.1080/17429145.2012.680077 Go to original source...
    12. EPPO. 2013. PM 7/119 (1) Nematode extraction. EPPO Bulletin, 43(3): 471-495. Go to original source...
    13. FIGUEROA-LÓPEZ, A. M., CORDERO-RAMÍREZ, J. D., MARTÍNEZ-ÁLVAREZ, J. C., LÓPEZ-MEYER, M., LIZÁRRAGA-SÁNCHEZ, G. J., FÉLIX-GASTÉLUM, R., CASTRO-MARTÍNEZ, C. and MALDONADO-MENDOZA, I. E. 2016. Rhizospheric bacteria of maize with potential for biocontrol of Fusarium verticillioides. SpringerPlus, 5(1): 330-341. DOI: 10.1186/s40064-016-1780-x Go to original source...
    14. GADEBERG, O., ORSKOV, I. and RHODES, J. M. 1983. Cytotoxic effect of an alpha-hemolytic Escherichia coli strain on human blood monocytes and granulocytes in vitro. Infection and Immunity, 41(1): 358-364. Go to original source...
    15. HACKENBERG, C., MUEHLKCHEN, A., FORGE, T. and VRAIN, T. 2000. Pseudomonas chlororaphis strain Sm3, bacterial antagonist of Pratylenchus penetrans. Journal of Nematology, 32(2): 183-189.
    16. HALLMANN, J., BERG, G. and SCHULZ, B. 2006. Isolation procedures for endophytic microorganisms. In: SCHULZ, B. J. E., BOYLE, C. J. C. and SIEBER, T. N. (Eds.). Microbial Root Endophytes. Springer. Go to original source...
    17. HALLMANN, J., MEKETE, T., SIKORA, R. and KIEWNICK, S. 2009. Endophytic bacteria from Ethiopian coffee plants and their potential to antagonise Meloidogyne incognita. Nematology, 11(1): 117-127. DOI: 10.1163/156854108X398462 Go to original source...
    18. HARNI, R. and MUNIF, A. 2012. he Use of endophytic biological agents to control of yellow disease in black pepper [in Indonesian: Pemanfaatan agens hayati endofit untuk mengendalikan penyakit kuning pada tanaman lada]. Jurnal Tanaman Industri dan Penyegar, 3(3): 201-206.
    19. IRMA, A., MERYANDINI, A. and RUPAEDAH, B. 2018. Biofungicide producing bacteria: an in vitro inhibitor of Ganoderma boninense. Hayati Journal of Biosciences, 25(4): 151-159. Go to original source...
    20. KHAN, A., WILLIAMS, K. L. and NEVALAINEN, H. K. 2004. Effects of Paecilomyces lilacinus protease and chitinase on the eggshell structures and hatching of Meloidogyne javanica juveniles. Biological Control, 31(3): 346-352. DOI: 10.1016/j.biocontrol.2004.07.011 Go to original source...
    21. KHAN, M. S., ZAIDI, A. and WANI, P. A. 2007. Role of phosphate-solubilizing microorganisms in sustainable agriculture-a review. Agronomy for Sustainable Development, 27(1): 29-43. DOI: 10.1051/agro:2006011 Go to original source...
    22. KLEMENT, Z. and GOODMAN, R. 1967. The hypersensitive reaction to infection by bacterial plant pathogens. Annual Review of Phytopathology, 5(1): 17-44. DOI: 10.1146/annurev.py.05.090167.000313 Go to original source...
    23. KUMAR, A., KUMAR, A., DEVI, S., PATIL, S., PAYAL, C. and NEGI, S. 2012. Isolation, screening and characterization of bacteria from rhizospheric soils for different plant growth promotion (PGP) activities: an in vitro study. Recent Research in Science and Technology, 4(1): 1-5.
    24. LODEWYCKX, C., VANGRONSVELD, J., PORTEOUS, F., MOORE, E. R., TAGHAVI, S., MEZGEAY, M. and DER LELIE, D. V. 2002. Endophytic bacteria and their potential applications. Critical Reviews in Plant Sciences, 21(6): 583-606. DOI: 10.1080/0735-260291044377 Go to original source...
    25. MARDHIANA, M., PRADANA, A. P., ADIWENA, M., SANTOSO, D., WIJAYA, R. and MURTILAKSONO, A. 2017. Use of endophytic bacteria from roots of Cyperus rotundus for biocontrol of Meloidogyne incognita. Biodiversitas, 18(4): 1308-1315. Go to original source...
    26. MEHTA, S. and NAUTIYAL, C. S. 2001. An efficient method for qualitative screening of phosphate-solubilizing bacteria. Current Microbiology, 43(1): 51-56. DOI: 10.1007/s002840010259 Go to original source...
    27. MOENS, M., PERRY, R. N. and STARR, J. L. 2009. Meloidogyne species-a diverse group of novel and important plant parasites. In: PERRY, R. N., MOENS, M. and STARR, J. L. (Eds.). Root-Knot Nematodes. CABI. Go to original source...
    28. NURDEBYANDARU, N., MUBARIK, N. R. and PRAWASTI, T. S. 2010. Chitinolytic bacteria isolated from chili rhizosphere: chitinase characterization and application as biocontrol for Aphis gossypii. Microbiology Indonesia, 4(3): 103-107. DOI: 10.5454/mi.4.3.1 Go to original source...
    29. PADGHAM, J. and SIKORA, R. 2007. Biological control potential and modes of action of Bacillus megaterium against Meloidogyne graminicola on rice. Crop Protection, 26(7): 971-977. DOI: 10.1016/j.cropro.2006.09.004 Go to original source...
    30. PRADANA, A. P., MUNIF, A. and SUPRAMANA, S. 2016. Endophytic bacteria from root of several plants as biocontrol agents of the root-knot nematode Meloidogyne incognita on tomato [in Indonesian: Bakteri endofit asal berbagai akar tanaman sebagai agens pengendali nematoda puru akar Meloidogyne incognita pada tomat]. Jurnal Fitopatologi Indonesia, 12(3): 75-82. Go to original source...
    31. REINHOLD-HUREK, B. and HUREK, T. 2011. Living inside plants: bacterial endophytes. Current Opinion in Plant Biology, 14(4): 435-443. DOI: 10.1016/j.pbi.2011.04.004 Go to original source...
    32. RICH, J., BRITO, J., KAUR, R. and FERRELL, J. 2009. Weed species as hosts of Meloidogyne: a review. Nematropica, 39(2): 157-185.
    33. ROSENBLUETH, M. and MARTÍNEZ-ROMERO, E. 2006. Bacterial endophytes and their interactions with hosts. Molecular Plant-Microbe Interactions, 19(8): 827-837. DOI: 10.1094/MPMI-19-0827 Go to original source...
    34. RYAN, R. P., GERMAINE, K., FRANKS, A., RYAN, D. J. and DOWLING, D. N. 2008. Bacterial endophytes: recent developments and applications. FEMS Microbiology Letters, 278(1): 1-9. DOI: 10.1111/j.1574-6968.2007.00918.x Go to original source...
    35. SHORESH, M., HARMAN, G. E. and MASTOURI, F. 2010. Induced systemic resistance and plant responses to fungal biocontrol agents. Annual Review of Phytopathology, 48: 21-43. DOI: 10.1146/annurev-phyto-073009-114450 Go to original source...
    36. SOKOL, P. A., OHMAN, D. E. and IGLEWSKI, B. H. 1979. A more sensitive plate assay for detection of protease production by Pseudomonas aeruginosa. Journal of Clinical Microbiology, 9(4): 538-540. Go to original source...
    37. THAKKAR, A. and SARAF, M. 2015. Development of microbial consortia as a biocontrol agent for effective management of fungal diseases in Glycine max L. Archives of Phytopathology and Plant Protection, 48(6): 459-474. DOI: 10.1080/03235408.2014.893638 Go to original source...
    38. WALPOLA, B. C. and YOON, M.-H. 2012. Prospectus of phosphate solubilizing microorganisms and phosphorus availability in agricultural soils: A review. African Journal of Microbiology Research, 6(37): 6600-6605. Go to original source...
    39. WIRATNO, W., SYAKIR, M., SUCIPTO, I. and PRADANA, A. P. 2019. Isolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita. Biodiversitas, 20(3): 682-687. DOI: 10.13057/biodiv/d200310 Go to original source...
    40. ZAKIR, H. and BORA, B. 2009. Interrelationship of Meloidogyne incognita and Ralstonia solanacearum complex in brinjal. Indian Journal of Nematology, 39(1): 41-45.

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