Acta Univ. Agric. Silvic. Mendelianae Brun. 2019, 67(1), 179-187 | DOI: 10.11118/actaun201967010179

Probiotic Candidates Yeast Isolated from Dangke - Indonesian Traditional Fermented Buffalo Milk

Gemilang Lara Utama1, Siska Meliana1, Mohamad Djali1, Tri Yuliana1, Roostita L. Balia2
1 Faculty of Agro-Industrial Technology, Universitas Padjadjaran, Bandung, 45363, Indonesia
2 Faculty of Animal Husbandry, Universitas Padjadjaran, Bandung, 45363, Indonesia

The aim of the study was to isolate and identify the yeast isolated from Dangke and its potential as probiotics. The purified isolates obtained were identified based on observations of macroscopic characteristics of colonies and microscopic cells. The ability as a probiotic yeast is obtained by testing the resistance towards acid conditions, bile salt resistance test and aggregation ability test against pathogenic bacteria using Salmonella sp. The yeast isolates were identified using the RapID Yeast Plus System. The isolation result was obtained D.10.3.d isolate that identified as Candida guilliermondii which showed probiotic characteristic. The yeast colony is round, cream-colored, smooth surfaces, low convex elevations and entire edges, capable of growing on mediums with the pH of 4, containing 1 % and 5 % of bile salts and having the ability to aggregate Salmonella sp. at 15, 60, and 180 minutes.

Keywords: Dangke, yeast, identification, isolation, probiotics
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This research / article's publication is supported by the United States Agency for International Development (USAID) through the Sustainable Higher Education Research Alliance (SHERA) Program for Universitas Indonesia's Scientific Modeling, Application, Research and Training for City-centered Innovation and Technology (SMART CITY) Project, Grant #AID-497-A1600004, Sub Grant #IIE-00000078-UI-1.

Received: August 17, 2018; Accepted: November 22, 2018; Published: February 28, 2019  Show citation

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Utama, G.L., Meliana, S., Djali, M., Yuliana, T., & Balia, R.L. (2019). Probiotic Candidates Yeast Isolated from Dangke - Indonesian Traditional Fermented Buffalo Milk. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 67(1), 179-187. doi: 10.11118/actaun201967010179
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    References

    1. ANNUK, H., HYNES, S.O., HIRMO, S., MIKELSAAR, M. and WADSTROM, T. 2001. Characterization and differentiation of lactobacilli by lectin typing. J. Med. Microbiol., 50(12): 1069-1074. DOI: 10.1099/0022-1317-50-12-1069 Go to original source...
    2. BHATIA, S.C. 2016. Food Biotechnology. New Delhi: Woodhead Publishing India Ltd.
    3. BHOWMIK, G. 2011. Analytical Techniques in Biotechnology. New Delhi: Tata McGraw Hill Education Private Ltd.
    4. BIJL, N., A. VELDE, V. D. and GROEN, A. K. 2009. Bile Acids and Their Role in Cholesterol Homeostasis. In: EHNHOLM C. (Eds.). Cellular Lipid Metabolism. Berlin, Heidelberg: Springer. Go to original source...
    5. BRINK, B. 2013. Urease test protocol. [Online]. American society for microbiology. Available at: http://www.microbelibrary.org/library/laboratorytest/2871-urease-test protocol [Accessed: 2018, August 21].
    6. CORCORAN, B. M., STANTON, C., FITZGERALD, G. F. and ROSS, R. P. 2005. Survival of probiotic in acidic environments is enhanced in the presence of metabolizable sugars. Appl. Environ. Microbiol., 71(6): 3060-3067. DOI: 10.1128/AEM.71.6.3060-3067.2005 Go to original source...
    7. CZERUCKA, D., PICHE, T. and RAMPAL, P. 2007. Review article: yeast as probiotics Saccharomyces boulardii. Aliment. Pharmacol. Therap., 26(6): 767-778. DOI: 10.1111/j.1365-2036.2007.03442.x Go to original source...
    8. DE SMET, I., HOORDE, L. V., WOESTYNE, M. V., CHRISTIAENS, H. and VERSTRAETE, W. 1995. The significance of bile salt hydrolytic activities of lactobacilli. J. App. Bacteriol., 79(3): 292-301. DOI: 10.1111/j.1365-2672.1995.tb03140.x Go to original source...
    9. DJIDE, M. N. and WAHYUDDIN, E. 2008. Isolasi Bakteri Asam Laktat dan Air Susu Ibu dan Potensinya dalam Penurunan Kadar Kolestrol secara In Vitro. Majalah Farmasi dan Farmakologi, 12(3): 73-78.
    10. DRABIKOVA, K., PERECKO, T., NOSAL, R., BAUEROVA, K., PONIST, S., MIHALOVA, D., KOGAN, G. and JANCINOVA, V., 2009. Glucomannan reduces neutrophil free radical production in vitro and in rats with adjuvant arthritis. Pharmacol. Res., 59(6): 399-403. DOI: 10.1016/j.phrs.2009.02.003 Go to original source...
    11. HALIM, C. N. and ZUBAIDAH, E. 2013. Probiotic Assay of Lactic Acid Bacteria as High Exopolysaccharides Producer from Sour Pickled Mustard (Brassica juncea). Jurnal Pangan dan Agroindustri, 1(1): 129-137.
    12. HARDININGSIH, R., NAPITUPULU, R. N. R. and YULINERY, T., 2006. Isolation and resistance test of several isolates of Lactobacillus in low pH. Biodiversitas, 7(1): 15-17. DOI: 10.13057/biodiv/d070105 Go to original source...
    13. HOLZAPFEL, W. H. 2005. Introduction to Prebiotics and Probiotics. In: GOKTEPE, I., JUNEJA, V.K., and AHMEDNA, M. (Eds.). Probiotics in Food Safety and Human Health. CRC Taylor & Francis Group. Go to original source...
    14. INDAH, H., PUTRI, F. and UTAMA, G. L. 2015. Preliminary studies of halophilic yeasts antimicrobial activities isolated from cocoa bean pulp towards E. coli and Salmonella spp. Int. J. on Adv. Sci. Eng. and Information Technology, 5(2): 107-109. DOI: 10.18517/ijaseit.5.2.498 Go to original source...
    15. IRIANTO, A. 2008. Efek Antibakteri Aloe Vera Terhadap Porphyromonas gingivalis In Vitro (Perbandingan Metode Ekstraksi Maserasi dan Infundasi). Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia.
    16. JIN, Y. L., RITCEY, L. L. and SPEERS, R. A. 2001. Effect of cell surface hydrophobicity, charge and zymolectin density on the flocculation of Saccharomyces cerevisiae. J. Am. Soc. Brew. Chem., 59(1): 1-9. Go to original source...
    17. KANMANI, P., KUMAR, R. S., YUVARAJ, N., PAARI, K. A., PATTUKUMAR, V. and ARUL, V. 2010. Comparison of antimicrobial activity of probiotic bacterium Streptococcus phocae P180, Enterococcus faecium MC13 and Carnobacterium divergence against the fish pathogen. World Journal of Dairy and Food Sciences, 5(2): 145-151.
    18. KHOTIMAH, K., BALIA, R., CHAERUNNISA, H., SAFITRI, R., UTAMA, G. L., SAPUTRA, M. I. and SYARA, A. 2015. Isolation and Identification Probiotic Candidates Bacteria from Crossbreed Fries Holland (CFH) Colostrum that Grown on Nutrient Agar with Olive Oil and Casein Enrichment. Adv. J. of Food Sci. and Technol., 8(1): 22-26. DOI: 10.19026/ajfst.8.1458 Go to original source...
    19. KIMOTO, H., OHMOMO, S. and OKAMOTO, T. 2002. Enhancement of Bile Tolerance in Lactococci by Tween 80. J. Of Applied Microbiology, 92(1): 41-46. DOI: 10.1046/j.1365-2672.2002.01505.x Go to original source...
    20. LEBOFFE, M. J. and PIERCE, B. E. 2011. A Photographic Atlas for The Microbiology Laboratory. 4th Edition. Morton Publishing Company.
    21. MIADOKOVA, E., SVIDOVA, S., VLCKOVA, V., DUHOVA, V., NADOVA, S. RAUKO, P. and KOGAN, G. 2006. Diverse biomodulatory effects of glucomannan from Candida utilis. Toxicol. In Vitro, 20(5): 649-657. DOI: 10.1016/j.tiv.2005.12.001 Go to original source...
    22. NEHAL, N., 2013. Knowledge of traditional fermented food products harbored by the tribal folks of Indian Himalayan belt. Int. J. Agric. Food Sci. Technol., 4(5): 401-414.
    23. ORLEAN, P. 2012. Architecture and biosynthesis of the Saccharomyces cerevisiae cell wall. Genetics, 192(3): 775-818. DOI: 10.1534/genetics.112.144485 Go to original source...
    24. PACHECO, K. C., DEL TORO, G. V., MARTINEZ, F. R. and DURAN-PARAMO, E. 2010. Viability of Lactobacillus delbrueckii under human gastrointestinal conditions simulated in vitro. Am. J. Agric. Biol. Sci., 5(1): 37-42. DOI: 10.3844/ajabssp.2010.37.42 Go to original source...
    25. PEREZ-SOTELO, L. S., TALAVERA-ROJAS, M., MONROY-SALAZAR, H. G., LAGUNAS-BERNABE, S., CUARON-IBARGUENGOYTIA, J. A., JIMENEZ, R. M. and VAZQUEZ-CHAGOYAN, J. C. 2005. In vitro evaluation of the binding capacity of Saccharomyces cerevisiae Sc47 to adhere to the wall of Salmonella spp. Rev. Latinoam Microbiol., 47(3-4): 70-75.
    26. POGACIC, T., SINKO, S., ZAMBERLIN, S. and SAMARZIJA, D., 2013. Micro-biota of kefir grains. Mljekarstvo, 63(1): 3-14.
    27. PUSPAWATI, N. N. and ARIHANTANA, N. M. I. H. 2016. Viability of Lactic Acid Bacteria Isolated from Kombucha Tea Against Low pH and Bile Salt. Media Ilmiah Teknologi Pangan, 3(1): 18-25.
    28. RAHMAN, S. 2014. Studi Pengembangan Dangke sebagai Pangan Lokal Unggulan dari Susu di Kabupaten Enrekang. J. Apl. Tek. Pangan., 3(2): 41-45
    29. RICKARD, A. K., LEACH, S. A., BUSWELL, C. M., HIGH, N. J. and HANDLEY, P.S. 2000. Coaggregation between aquatic bacteria is mediated by specific growth phase-dependent lectinsaccharide interactions. Appl. Environ. Microbiol., 66(1): 431-434 DOI: 10.1128/AEM.66.1.431-434.2000 Go to original source...
    30. ROOSTITA, R. and FLEET, G. H., 1999. Growth of Yeasts Isolated from Cheeses on Organic Acids in the Presence of Sodium Chloride. Food Technol. and Biotechnol., 37: 73-80.
    31. SALEN, G. and BATTA, A. K. 2004. Bile Formation. In: Encyclopedia of Gastroenterology. Elsevier Inc, USA. Go to original source...
    32. SALMINEN, S., GORBACH, S., KUN LEE, Y. and BENNO, Y. 2004. Human studies on probiotics: What is scientifically proven today? In: SALMINEN, S., VON WRIGHT, A. and OUWEHAND, A. (Eds.). Lactic Acid Bacteria: Microbiological and Functional Aspect Third Edition. New York: Marcel Dekker Inc. Go to original source...
    33. SIMOVA, E., BESHKOVA, D., ANGELOV, E., HRISTOZOVA, T., FRENGOVA, G. and SPASOV, Z., 2002. Lactic acid bacteria and yeasts in kefir grains and kefir made from them. J. Ind. Microbiol. Biotechnol., 28(1): 1-6. DOI: 10.1038/sj/jim/7000186 Go to original source...
    34. SHAH, N. P. 2007. Functional cultures and health benefits. Int. Dairy J., 17(11): 1262-1277. DOI: 10.1016/j.idairyj.2007.01.014 Go to original source...
    35. SOHLIYA, I., JOSHI, S. R., BHAGOBATY, R. K. and KUMAR, R., 2009. Tungrymbai - a traditional fermented soybean food of the ethnic tribes of Meghalaya. Indian J. Tradit. Knowl., 8(4): 559-561.
    36. SURONO, I. S. 2004. Probiotic Susu Fermentasi dan Kesehatan. Yayasan Pengusaha Makanan dan Minuman Seluruh Indonesia, Jakarta.
    37. SUSANTI, I., KUSUMANINGTYAS, R. W. and ILLANINGTYAS, F. 2007. Probiotic Characteristics of Lactic Acid Bacteria as Candidate for Functional Food. J. Teknol. Indust. Pangan, 18(2): 89-95.
    38. USMIATI, S., BROTO, W. and SETIYANTO, H. 2011. Characteristic of cow milk dadih using starter of probiotic of lactic acid bacteria. JITV, 16(2): 140-152.
    39. UTAMA, G. L., KURNANI, T. B. A., SUNARDI and BALIA, R. L. 2016. The isolation and identification of stress tolerance ethanolfermenting yeasts from mozzarella cheese whey. Int. J. on Adv. Sci. Eng. and Information Technology, 6(2): 252-257. DOI: 10.18517/ijaseit.6.2.752 Go to original source...
    40. VICKOVA, V., DUHOVA, V., SVIDOVA, S., FARKASSOVA, A., KAMASOVA, S., VLCEK, D., KOGAN, G., RAUKO, P. and MIADOKOVA, E. 2004. Antigenotoxic potential of glucomannan on four model test systems. Cell Biol. Toxicol., 20(6): 325-332. DOI: 10.1007/s10565-004-0089-7 Go to original source...
    41. VINE, N. G., DUKES, W. D. and KAISER, H., 2006. Probiotics in marine larviculture. FEMS Microbiol. Rev., 30(3): 404-27 DOI: 10.1111/j.1574-6976.2006.00017.x Go to original source...
    42. WAHYUNI, A. 2006. Genetic analysis of S. agalactiae caused subclinical mastitis in dairy cattle by Pulsed Field Gel Electrophoresis (PFGE). Journal of Sain Veteriner, 26(1): 110-118.
    43. YANG, Y. L., HUNG, C. C., WANG, A. H., TSENG, F. C., LEAW, S. N., TSENG, Y. T., SU, C. L., CHEN, H. T., LAUDERDALE, T. L. and LO, H. J. 2010. Oropharyngeal colonization of HIV-infected outpatients in Taiwan by yeast pathogens. J. Clin. Microbiol., 48(7): 2609-12. DOI: 10.1128/JCM.00500-10 Go to original source...

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