Acta Univ. Agric. Silvic. Mendelianae Brun. 2019, 67(1), 121-131 | DOI: 10.11118/actaun201967010121

Evaluation of Presence and Concentration of PPV in Rootstocks Derived from Prunus davidiana (Carr.) Franch

Petra Pavelková, Tomáš Kiss, Tomáš Nečas
Department of Fruit Growing, Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech Republic

Evaluation of the presence and concentration of PPV (Plum pox virus) in selected rootstocks was carried out in 2016-2017. For the purpose of the experiment we used rootstocks derived from crossbreeding of Prunus davidiana (Carr.) Franch, such as Cadaman and Barrier, and also a P. davidiana seedling. Peach seedling rootstock GF-305 was used as a control. The rootstocks were inoculated artificially with PPV strain M (Marcus). Both the rootstock and the inoculum were tested for presence of the virus by a serological method - semiquantitative DAS-ELISA test and molecular methods - RT-PCR, real-time RT-PCR and RT-LAMP. During the growing season the plants were evaluated for symptom intensity by using a scoring scale. The results show interdependency between symptom intensity and the amount of PPV in leaves, with DAS-ELISA test giving less positive samples than RT-PCR. The RT-LAMP and real-time RT-PCR methods were capable of revealing low concentrations of the virus even in symptom-free plants. The lowest PPV concentrations of all the four rootstocks were detected by real-time RT-PCR in P. davidiana. The highest PPV concentrations were detected in Barrier rootstock. In inocula, the lowest concentration was found in the inocula on Cadaman rootstock, whereas the highest PPV concentration was detected in the inocula inoculated on Barrier rootstock.

Keywords: sharka, stone fruit, symptoms, David's peach, DAS-ELISA, RT-PCR, RT-LAMP, real-time RT-PCR
Grants and funding:

This research was funded by NAZV project QJ1510081 by the Ministry of Agriculture of the Czech Republic and with a financial support of the Mendel university Internal grant agency (IGA) from project [SP5170911].

Received: July 25, 2018; Accepted: October 16, 2018; Published: February 28, 2019  Show citation

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Pavelková, P., Kiss, T., & Nečas, T. (2019). Evaluation of Presence and Concentration of PPV in Rootstocks Derived from Prunus davidiana (Carr.) Franch. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 67(1), 121-131. doi: 10.11118/actaun201967010121
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