Acta Univ. Agric. Silvic. Mendelianae Brun. 2018, 66(5), 1191-1197 | DOI: 10.11118/actaun201866051191

Significance of Sexual Reproduction of Phytophthora infestans in the Czech Republic

Jana Mazáková, Miloslav Zouhar, Pavel Ryąánek
Department of Plant Protection, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Prague, Czech Republic

A total of 187 naturally late blight-diseased potato leaves were sampled from 31 sites and five regions of the Czech Republic during the growing season in 2012-2014 and 2016 and examined microscopically for the ability of Phytophthora infestans to produce oospores in infected leaves under field conditions. Although the occurrence of the A1 and A2 mating types required for sexual reproduction of P. infestans was previously confirmed in the Czech Republic, no oospores were detected in this study. To study the effect of temperature on the survival of oospores produced from crosses of three pairs of P. infestans isolates, oospores in leaf discs and agar were exposed to temperatures ranging from -24 to 10 °C and then evaluated for viability with the plasmolysis test. Oospore viability ranged from 13.02 to 63.90 % and from 7.77 to 63.37 % for oospores produced in agar and leaf discs, respectively, with the highest frequencies of viable oospores occurring at 4 and 10 °C. To determine whether oospores may survive under field conditions, agar plates with oospores were buried in soil in Prague-Suchdol and Svitavy for seven months during the overwintering period (October-May) in 2011-2015. There were statistically significant differences in oospore viability examined by the plasmolysis test between the localities, and oospore viability ranged from 29 to 43 % and from 15 to 44 % in Prague-Suchdol and Svitavy, respectively.

Keywords: potato late blight, mating type, oospores, overwintering
Grants and funding:

Supported by the Ministry of Agriculture of the Czech Republic, Project No. QJ1210305.

Published: October 29, 2018  Show citation

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Mazáková, J., Zouhar, M., & Ryąánek, P. (2018). Significance of Sexual Reproduction of Phytophthora infestans in the Czech Republic. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 66(5), 1191-1197. doi: 10.11118/actaun201866051191
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    References

    1. ANDERSSON, B., SANDSTRÖM, M. and STRÖMBERG, A. 1998. Indications of soil borne inoculum of Phytophthora infestans. Potato Research, 41(4): 305-310. DOI: 10.1007/BF02358962 Go to original source...
    2. COHEN, Y., FARKASH, S., BAIDER, A. and SHAW, D. S. 2000. Sprinkling irrigation enhances production of oospores of Phytophthora infestans in field-grown crops of potato. Phytopathology, 90(10): 1105-1111. DOI: 10.1094/PHYTO.2000.90.10.1105 Go to original source...
    3. COHEN, Y., FARKASH, S., RESHIT, Z. and BAIDER, A. 1997. Oospore production of Phytophthora infestans in potato and tomato leaves. Phytopathology, 87(2): 191-196. DOI: 10.1094/PHYTO.1997.87.2.191 Go to original source...
    4. DRENTH, A., JANSSEN, E. M. and GOVERS, F. 1995. Formation and survival of oospores of Phytophthora infestans under natural conditions. Plant Pathology, 44(1): 86-94. DOI: 10.1111/j.1365-3059.1995.tb02719.x Go to original source...
    5. DRENTH, A., TAS, I. C. Q. and GOVERS, F. 1994. DNA fingerprinting uncovers a new sexually reproducing population of Phytophthora infestans in the Netherlands. European Journal of Plant Pathology, 100(2): 97-107. DOI: 10.1007/BF01876244 Go to original source...
    6. ETXEBERRIA, A., MENDARTE, S. and LARREGLA, S. 2011. Determination of viability of Phytophthora capsici oospores with the tetrazolium bromide staining test versus a plasmolysis method. Revista Iberoamericana de Micologia, 28(1): 43-49. DOI: 10.1016/j.riam.2010.11.005 Go to original source...
    7. FLIER, W. G., GRÜNWALD, N. J., FRY, W. E. and TURKENSTEEN, L. J. 2001. Formation, production and viability of oospores of Phytophthora infestans from potato and Solanum demissum in the Toluca Valley, central Mexico. Mycological Research, 105(8): 998-1006. DOI: 10.1016/S0953-7562(08)61958-9 Go to original source...
    8. FRY, W. 2008. Phytophthora infestans: the plant (and R gene) destroyer. Molecular Plant Pathology, 9(3), 385-402. DOI: 10.1111/j.1364-3703.2007.00465.x Go to original source...
    9. FRY, W. E., BIRCH, P. R. J., JUDELSON, H. S., GRÜNWALD, N. J., DANIES, G., EVERTS, K. L., GEVENS, A. J., GUGINO, B. K., JOHNSON, D. A., JOHNSON, S. B., MCGRATH, M. T., MYERS, K. L., RISTAINO, J. B., ROBERTS, P. D., SECOR, G. and SMART, C. D. 2015. Five reasons to consider Phytophthora infestans a reemerging pathogen. Phytopathology, 105(7): 966-981. DOI: 10.1094/PHYTO-01-15-0005-FI Go to original source...
    10. FRY, W. E. and GOODWIN, S. B. 1997a. Re-emergence of potato and tomato late blight in the United States. Plant Disease, 81(12): 1349-1357. DOI: 10.1094/PDIS.1997.81.12.1349 Go to original source...
    11. FRY, W. E. and GOODWIN, S. B. 1997b. Resurgence of the Irish potato famine fungus. BioScience, 47(6): 363-371. DOI: 10.2307/1313151 Go to original source...
    12. FRY, W. E., MCGRATH, M. T., SEAMAN, A., ZITTER, T. A., MCLEOD, A., DANIES, G., SMALL, I. M., MYERS, K., EVERTS, K., GEVENS, A. J., GUGINO, B. K., JOHNSON, S. B., JUDELSON, H., RISTAINO, J., ROBERTS, P., SECOR, G., SEEBOLD, K., SNOVER-CLIFT, K., WYENANDT, A., GRÜNWALD, N. J. and SMART, C. D. 2013. The 2009 late blight pandemic in the eastern United States - causes and results. Plant Disease, 97(3): 296-306. DOI: 10.1094/PDIS-08-12-0791-FE Go to original source...
    13. GAVINO, P. D., SMART, C. D., SANDROCK, R. W., MILLER, J. S., HAMM, P. B., T., Y. L., DAVIS, R. M. and FRY, W. E. 2000. Implications of sexual reproduction for Phytophthora infestans in the United States: generation of an aggressive lineage. Plant Disease, 84(7): 731-735. DOI: 10.1094/PDIS.2000.84.7.731 Go to original source...
    14. GOODWIN, S. B. 1997. The population genetics of Phytophthora. Phytopathology, 87(4): 462-473. DOI: 10.1094/PHYTO.1997.87.4.462 Go to original source...
    15. GOODWIN, S. B., COHEN, B. A. and FRY, W. E. 1994. Panglobal distribution of a single clonal lineage of the Irish potato famine fungus. Proceedings of the National Academy of Sciences of the United States of America, 91(24): 11591-11595. DOI: 10.1073/pnas.91.24.11591 Go to original source...
    16. GÖTZ, E. 1991. Untersuchungen zum Auftreten des A2-Paarungstyps bei Phytophthora infestans (Mont.) de Bary in Ostdeutschland. Potato Research, 34(3): 233-237. DOI: 10.1007/BF02360494 Go to original source...
    17. HOHL, H. R. and ISELIN, K. 1984. Strains of Phytophthora infestans from Switzerland with A2 mating type behaviour. Transactions of the British Mycological Society, 83(3): 529-530. DOI: 10.1016/S0007-1536(84)80057-1 Go to original source...
    18. JUDELSON, H. S. 2007. Sexual reproduction in plant pathogenic oomycetes: biology and impact on disease. In: HEITMAN, J., KRONSTAD, J., TAYLOR, J. and CASSELTON, L. (Eds.). Sex in Fungi. Washington, DC., USA: ASM Press.
    19. KAMOUN, S., FURZER, O., JONES, J. D. G., JUDELSON, H. S., ALI, G. S., DALIO, R. J. D., ROY, S. G., SCHENA, L., ZAMBOUNIS, A., PANABIČRES, F., CAHILL, D., RUOCCO, M., FIGUEIREDO, A., CHEN, X.-R., HULVEY, J., STAM, R., LAMOUR, K., GIJZEN, M., TYLER, B. M., GRÜNWALD, N. J., MUKHTAR, M. S., TOMÉ, D. F. A., TÖR, M., VAN DEN ACKERVEKEN, G., MCDOWELL, J., DAAYF, F., FRY, W. E., LINDQVIST-KREUZE, H., MEIJER, H. J. G., PETRE, B., RISTAINO, J., YOSHIDA, K., BIRCH, P. R. J. and GOVERS, F. 2015. The Top 10 oomycete pathogens in molecular plant pathology. Molecular Plant Pathology, 16(4): 413-434. DOI: 10.1111/mpp.12190 Go to original source...
    20. KNAPOVA, G. and GISI, U. 2002. Phenotypic and genotypic structure of Phytophthora infestans populations on potato and tomato in France and Switzerland. Plant Pathology, 51(5): 641-653. DOI: 10.1046/j.1365-3059.2002.00750.x Go to original source...
    21. LI, Y., COOKE, D. E. L., JACOBSEN, E. and VAN DER LEE, T. 2013. Efficient multiplex simple sequence repeat genotyping of the oomycete plant pathogen Phytophthora infestans. Journal of Microbiological Methods, 92(3): 316-322. DOI: 10.1016/j.mimet.2012.11.021 Go to original source...
    22. MAYTON, H., SMART, C. D., MORAVEC, B. C., MIZUBUTI, E. S. G., MULDOON, A. E. and FRY, W. E. 2000. Oospore survival and pathogenicity of single oospore recombinant progeny from a cross involving US-17 and US-8 genotypes of Phytophthora infestans. Plant Disease, 84(11): 1190-1196. DOI: 10.1094/PDIS.2000.84.11.1190 Go to original source...
    23. MAZÁKOVÁ, J., TÁBORSKÝ, V., ZOUHAR, M., RY©ÁNEK, P., HAUSVATER, E. and DOLE®AL, P. 2006. Occurrence and distribution of mating types A1 and A2 of Phytophthora infestans (Mont.) de Bary in the Czech Republic. Plant Protection Science, 42(2): 41-48. DOI: 10.17221/2697-PPS Go to original source...
    24. MAZÁKOVÁ, J., ZOUHAR, M., RY©ÁNEK, P., TÁBORSKY, V., HAUSVATER, E. and DOLE®AL, P. 2010. Mating type distribution of Phytophthora infestans (Mont.) de Bary in the Czech Republic in 2007 and 2008. Plant Protection Science, 46(3): 89-97. DOI: 10.17221/49/2009-PPS Go to original source...
    25. MEDINA, M. V. and PLATT, H. W. 1999. Viability of oospores of Phytophthora infestans under field conditions in northeastern North America. Canadian Journal of Plant Pathology, 21(2): 137-143. DOI: 10.1080/07060669909501204 Go to original source...
    26. NIEDERHAUSER, J. S. 1991. Phytophthora infestans: the Mexican connection. In: LUCAS, J. A., SHATTOCK, R. C., SHAW, D. S. and COOKE, L. R. (Eds.). Phytophthora. Cambridge, GB: British Mycological Society, Cambridge University Press.
    27. OLIVA, R. F., ERSELIUS, L. J., ADLER, N. E. and FORBES, G. A. 2002. Potential of sexual reproduction among host-adapted populations of Phytophthora infestans sensu lato in Ecuador. Plant Pathology, 51(6): 710-719. DOI: 10.1046/j.1365-3059.2002.00784.x Go to original source...
    28. PIPE, N. D., AZCOITIA, V. and SHAW, D. S. 2000. Self-fertility in Phytophthora infestans: heterokaryons segregate several phenotypes. Mycological Research, 104(6): 676-680. DOI: 10.1017/S0953756299001847 Go to original source...
    29. PITTIS, J. E. and SHATTOCK, R. C. 1994. Viability, germination and infection potential of oospores of Phytophthora infestans. Plant Pathology, 43(2): 387-396. DOI: 10.1111/j.1365-3059.1994.tb02700.x Go to original source...
    30. SEDLÁK, P., MAZÁKOVÁ, J., SEDLÁKOVÁ, V., RY©ÁNEK, P., VEJL, P. and DOLE®AL, P. 2017. Virulence and mating type of Phytophthora infestans isolates in the Czech Republic. Scientia Agriculturae Bohemica, 48(4): 185-192. DOI: 10.1515/sab-2017-0025 Go to original source...
    31. SUJKOWSKI, L. S., GOODWIN, S. B., DYER, A. T. and FRY, W. E. 1994. Increased genotypic diversity via migration and possible occurrence of sexual reproduction of Phytophthora infestans in Poland. Phytopathology, 84(2): 201-207. DOI: 10.1094/Phyto-84-201 Go to original source...
    32. SUTHERLAND, E. D. and COHEN, S. D. 1983. Evaluation of tetrazolium bromide as a vital stain for fungal oospores. Phytopathology, 73(11): 1532-1535. DOI: 10.1094/Phyto-73-1532 Go to original source...
    33. TURKENSTEEN, L. J., FLIER, W. G., WANNINGEN, R. and MULDER, A. 2000. Production, survival and infectivity of oospores of Phytophthora infestans. Plant Pathology, 49(6): 688-696. DOI: 10.1046/j.1365-3059.2000.00515.x Go to original source...
    34. YOSHIDA, K., SCHUENEMANN, V. J., CANO, L. M., PAIS, M., MISHRA, B., SHARMA, R., LANZ, C., MARTIN, F. N., KAMOUN, S., KRAUSE, J., THINES, M., WEIGEL, D. and BURBANO, H. A. 2013. The rise and fall of the Phytophthora infestans lineage that triggered the Irish potato famine. eLife, 2: e00731. DOI: 10.7554/eLife.00731 Go to original source...
    35. YUEN, J. E. and ANDERSSON, B. 2013. What is the evidence for sexual reproduction of Phytophthora infestans in Europe? Plant Pathology, 62(3): 485-491. DOI: 10.1111/j.1365-3059.2012.02685.x Go to original source...

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