Acta Univ. Agric. Silvic. Mendelianae Brun. 2017, 65(1), 67-72 | DOI: 10.11118/actaun201765010067

The Issues of Apricot (Prunus Armeniaca L.) Micropropagation

Martina Kudělková, Radka Pavelková, Eva Ondrušiková, Miroslav Vachůn
Mendeleum-Institute of Genetics, Faculty of Horticulture, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic

The effect of four modified mediums for apricot multiplication was observed in this study. A total number of 1864 single nodes of 20 Prunus armeniaca L.varieties were established. Explants surface was disinfected with 0.2 % mercuric chloride for 5 minutes. MS (1962) medium with 0.5 mg.l-1 BA, 0.01 mg.l-1 NAA and 0.5 mg.l-1 GA3 was used as a medium for primary culture. 'Velkopavlovická', 'Bergeron', genotype 1128 and genotype LE 2927 Š9 were successfully transferred to aseptic conditions and multiplied. Modified MS medium (1962), DKW/Juglans medium, Quoirin, Lepoivre (1977) medium and Marino et al. (1991) medium were used for multiplication. Modified MS medium and modified DKW/Juglans medium were not suitable for apricot multiplication at all and explants did not grow. The best results were observed in the case of Quoirin, Lepoivre (1977) medium with 0.4 mg.l-1 BA and 0.01 mg.l-1 NAA. Young plants multiplied well, were fresh and vital and no damage was observed. The highest number of new shoots was observed in the case of Marino et al. (1991) medium. The average growth of new shoots after the last passaging was 600 %, rate 7.33 (Velkopavlovická); 566 %, rate 7.0 (Bergeron); 475 %, rate 6.25 (1128) and 483 %, rate 6.33 (LE 2927 Š9)). However, new shoots in clusters were too dense and stunted and this medium is not recommended for apricot multiplication.

Keywords: Prunus armeniaca, in vitro propagation, culture medium, plant grow regulators, single node culture, shoot regeneration, multiplication
Grants and funding:

This paper was supported by the NAZV project QJ1210175: Research and development of standard methodic procedures of recovering of fruit trees and grapevines by chemotherapy of in vitro cultures for certification system of healthy status of planting material is gratefully acknowledged.

Prepublished online: February 28, 2017; Published: March 1, 2017  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Kudělková, M., Pavelková, R., Ondrušiková, E., & Vachůn, M. (2017). The Issues of Apricot (Prunus Armeniaca L.) Micropropagation. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 65(1), 67-72. doi: 10.11118/actaun201765010067
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. BRISON, M., DE BOUCAUD, M. T., PIERRONNET, A. and DOSBA, F. 1997. Effect of cryopreservation on the sanitary state of a cv Prunus rootstock experimentally contaminated with Plum Pox Potyvirus. Plant Science, 123(1): 189 - 196. DOI: 10.1016/S0168-9452(97)04581-0 Go to original source...
    2. ERREA, P., GARAY, L. and MARÍN, J. A. 2001. Early detection of graft incompatibility in apricot (Prunus armeniaca) using in vitro techniques. Physiologia Plantarum, 112(1): 135 - 141. DOI: 10.1034/j.1399-3054.2001.1120118.x Go to original source...
    3. ESCALETTES, V. and DOSBA, F. 1993. In vitro adventitious shoot regeneration from leaves of Prunus spp. Plant Science, 90(2): 201 - 209. DOI: 10.1016/0168-9452(93)90241-Q Go to original source...
    4. GAGO, J., PÉREZ-TORNERO, O., LANDÍN, M., BURGOS, L. and GALLEGO, P. P. 2011. Improving knowledge of plant tissue culture and media formulation by neurofuzzy logic: a practical case of data mining using apricot databases. Journal of plant physiology, 168(15): 1858 - 1865. DOI: 10.1016/j.jplph.2011.04.008 Go to original source...
    5. HAUPTMANOVÁ, A. and POLÁK, J. 2011. The elimination of Plum pox virus in plum cv. Bluefree and apricot cv. Hanita by chemotherapy of in vitro cultures. Hort. Sci.(Prague), 38: 49 - 53. DOI: 10.17221/10/2010-HORTSCI Go to original source...
    6. JAIN, S. M. and HÄGGMAN, H. (eds.). 2007. Protocols for micropropagation of woody trees and fruits. Springer Science & Business Media.
    7. KOUBOURIS, G. and VASILAKAKIS, M. 2006. Improvement of in vitro propagation of apricot cultivar 'Bebecou'. Plant cell, tissue and organ culture, 85(2): 173 - 180. DOI: 10.1007/s11240-005-9066-y Go to original source...
    8. KRAMARENKO, L. A. 1997. Micropropagation of Apricot and Field Performance of In Vitro Propagated Plants. In: XI International Symposium on Apricot Culture, 488: 417 - 420. Go to original source...
    9. KŘIŽAN, B., ONDRUŠIKOVÁ, E., MOUDRÁ, J., EICHMEIER, A. and HOLLEINOVÁ, V. 2011. Elimination of apple chlorotic leafspot virus on peaches and apricots by applying chemotherapy. Zahradnictví, 10(12): 18 - 19.
    10. MARINO, G., BERTAZZA, G., MAGNANINI, E., and ALTAN, E. D. 1993. Comparative effects of sorbitol and sucrose as main carbon energy sources in micropropagation of apricot. Plant Cell Tissue and Organ Culture, 34: 235 - 244. DOI: 10.1007/BF00029712 Go to original source...
    11. MARINO, G., MAGNANINI, E., BATTISTINI, S. and RIGHETTI, B. 1991. Effect of Hormones and Main Carbon Energy Sources on In Vitro Propagation of Apricot (Prunus armeniaca L.) cvs. 'San Castrese' and 'Portici'. Acta Hort., 79(2): 335 - 362. Go to original source...
    12. MOUDRÁ J., ONDRUŠIKOVÁ E. and KŘIŽAN B. 2012. Mikropropagation of apricot cultivars. Úroda, 60 (9): 53 - 56.
    13. MURASHIGE, T. and SKOOG, F. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant, 15(3): 473 - 497. DOI: 10.1111/j.1399-3054.1962.tb08052.x Go to original source...
    14. PEIXE, A., BARROSO, J., POTES, A. and PAIS, M. S. 2004. Induction of haploid morphogenic calluses from in vitro cultured anthers of Prunus armeniaca cv. 'Harcot'. Plant cell, tissue and organ culture, 77(1): 35 - 41. DOI: 10.1023/B:TICU.0000016498.95516.e6 Go to original source...
    15. PÉREZ-TORNERO, O., BURGOS, L. and EGEA, J. 1999. Introduction and establishment of apricot in vitro through regeneration of shoots from meristem tips. In Vitro Cellular & Developmental Biology-Plant, 35(3): 249 - 253. DOI: 10.1007/s11627-999-0087-9 Go to original source...
    16. PÉREZ-TORNERO, O., EGEA, J., OLMOS, E. and BURGOS, L. 2001. Control of hyperhydricity in micropropagated apricot cultivars. In Vitro Cellular & Developmental Biology-Plant, 37(2): 250 - 254. DOI: 10.1007/s11627-001-0044-8 Go to original source...
    17. PÉREZ-TORNERO, O., EGEA, J., VANOOSTENDE, A. and BURGOS, L. 2000. Assessment of factors affecting adventitious shoot regeneration from in vitro cultured leaves of apricot. Plant Science, 158(1): 61 - 70. DOI: 10.1016/S0168-9452(00)00303-4 Go to original source...
    18. QUOIRIN M. and LEPOIVRE P. 1977. Improved media for in vitro culture of Prunus sp. Acta Hort., 7: 437 - 442. DOI: 10.17660/ActaHortic.1977.78.54 Go to original source...
    19. YILDIRIM, H., AHMET, O. N. A. Y., TILKAT, E. and AKTÜRK, Z. 2011. Micropropagation of the apricot (Prunus armeniaca L.) cv. Hacihaliloğlu by means of single node culture. Turkish Journal of Agriculture and Forestry, 35(1): 55 - 64. Go to original source...
    20. YILDIRIM, H., TILKAT, E., ONAY, A. and OZEN, H. Ç. 2007. In Vitro Embryo Culture of Apricot, Prunus armeniaca L. cv. Hacihaliloğlu. International Journal of Science & Technology, 2(2): 99 - 104.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY NC ND 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content