Acta Univ. Agric. Silvic. Mendelianae Brun. 2016, 64(4), 1173-1179 | DOI: 10.11118/actaun201664041173

Nitrogen and Phosphorus Availability Effect on Activity of Cellulolytic Microorganisms in Meadows

Antonín Kintl1, Adam Nawrath2, Jakub Elbl3, Ivan Tůma4, Marcela Muchová3, Martin Brtnický3, Jindřich Kynický3
1 Agricultural research Ltd. Zahradní 1, 664 41 Troubsko, Czech Republic
2 Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1, 613 00 Brno Czech Republic
3 Department of Geology and Pedology, Faculty of Forestry and Wood Technology, Mendel University in Brno, Czech Republic, Zemědělská 1, 613 00 Brno 13, Czech Republic
4 Department of Agrochemistry, Soil Science, Microbiology and Plant Nutrition, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1, 613 00 Brno Czech Republic

Meadows occupy more than 23 % of agricultural land in the Czech Republic and also represent the largest pool of carbon in soil organic matter. The organic material is an essential component of healthy soil. Decomposition of organic matter is a biological process, affected by high amount of N and P fertilizer applied in the second half of the twentieth century. This work presents the analysis of the effect of available nutrients nitrogen and phosphorus on the activity of cellulolytic microorganisms in permanent grassland of Sanguisorba-Festucetum comutatae association in the soil surface in the Protected Landscape Area of Žďárské Hills. Contents of available nutrients established by the Mehlich III method were measured ex situ. Data referring cellulose decomposition rates were obtained in situ using the standard mesh-bag method. In the field experiment, the highest loss of cellulose was recorded in variants with the addition of nutrients (

PK, 90N + PK and

180N + PK) with result 90.38 % on average. The difference was statistically significant, compared with the control variant without added nutrients 27.87 % (P < 0.05). The added amount of nitrogen in the variant 90 kg of N + PK per ha had no significant effect on loss of cellulose compared to PK variant, as well as variant 180 kg of N + PK per ha. From the results obtained, it is evident that the highest decomposition of cellulose was observed at variant with added phosphorus, compared to variant with nitrogen. Moreover, the effect of different intensity of grassland utilization was found: the amount of decomposed cellulose was higher in two variants (control and 90N + PK) of two cut system in comparison with the same variant in three cut system.

Keywords: available nutrients, meadows, cellulose decomposition, mesh-bag
Grants and funding:

This work was supported by National Agency for Agricultural Research (NAZV), project: The possibilities for retention of reactive nitrogen from agriculture in the most vulnerable infiltration area of water resources, registration no.: QJ 1220007. The work was supported by the IGA - Internal Agency Faculty of Agronomy MENDELU No. IP 28/2015.

Prepublished online: August 30, 2016; Published: September 1, 2016  Show citation

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Kintl, A., Nawrath, A., Elbl, J., Tůma, I., Muchová, M., Brtnický, M., & Kynický, J. (2016). Nitrogen and Phosphorus Availability Effect on Activity of Cellulolytic Microorganisms in Meadows. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 64(4), 1173-1179. doi: 10.11118/actaun201664041173
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