Acta Univ. Agric. Silvic. Mendelianae Brun. 2016, 64(4), 1167-1172 | DOI: 10.11118/actaun201664041167

Effect of Selenium, Zinc, Vitamin C and E on Boar Ejaculate Quality at Heat Stress

Pavel Horký1, Ladislav Zeman1, Jiří Skládanka1, Pavel Nevrkla2, Petr Sláma3
1 Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic
2 Department of Animal Breeding, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1, Brno, Czech Republic
3 Department of Animal Morphology, Physiology and Genetics, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1, Brno, Czech Republic

The aim of experiment was to test effect of selected antioxidants (selenium, zinc, vitamin C and E) to reduce the impact of heat stress at boars. In the experiment, boars of Duroc breed were tested. The first control group (n = 10) was not supplemented with antioxidants. The second experimental group (n = 10) was supplemented with antioxidants in the following quantities of 0.5 mg of selenium (seleno-methionine), 100 mg of zinc (zinc-methionine), 70 mg of vitamin E (alpha-tocopherol) and 350 mg of vitamin C (ascorbic acid) per kilogram of their feed. The experiment was carried out for 120 days and took place in summer (June to September). During the experiment, average and maximum daily temperatures, where boars were stabled, were monitored. Average daily temperature ranged from 12 to 28 °C. Maximum temperature during the day was from 13 to 32 °C. The evaluation of the semen quality has revealed increased number of abnormal spermatozoa in the control group of boars by 39 % (P < 0.05). There were observed no significant changes at other monitored parameters (ejaculate volume, total count of produced sperm, motility and sperm concentration). The results show that the addition of selenium, zinc, vitamin C and E may reduce the effect of heat stress to some extent at breeding boars.

Keywords: antioxidant, boars, ejaculate, heat stress
Grants and funding:

This project was funded from grants and IGA TP 2/2015: Effect of selenium on the quality of plant and animal production from the perspective of safety.

Prepublished online: August 30, 2016; Published: September 1, 2016  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Horký, P., Zeman, L., Skládanka, J., Nevrkla, P., & Sláma, P. (2016). Effect of Selenium, Zinc, Vitamin C and E on Boar Ejaculate Quality at Heat Stress. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis64(4), 1167-1172. doi: 10.11118/actaun201664041167
Download citation

References

  1. ECHEVERRÍA-ALONZO, S., SANTOS-RICALDE, R., CENTURIÓN-CASTRO, F., AKE-LÓPEZ, R., ALFARO-GAMBOA, M., RODRÍGUEZ- -BUENFIL, J. 2009. Effects of dietary selenium and vitamin e on semen quality and sperm morphology of young boars during warm and fresh season. Journal of Animal and Veterinary Advances, 8: 2311 - 2317.
  2. HANSEN, P. J. 2009. Effects of heat stress on mammalian reproduction. Philosophical Transactions of the Royal Society B: Biological Sciences, 364: 3341 - 3350. DOI: 10.1098/rstb.2009.0131 Go to original source...
  3. HORKY, P., JANCIKOVA, P., SOCHOR, J., HYNEK, D., CHAVIS, G. J., RUTTKAY-NEDECKY, B., CERNEI, N., ZITKA, O., ZEMAN L., ADAM, V., KIZEK R. 2012. Effect of Organic and Inorganic Form of Selenium on Antioxidant Status of Breeding Boars Ejaculate Revealed by Electrochemistry. Int. J. Electrochem. Sci., 7: 9643 - 9657. Go to original source...
  4. HORKY, P., RUTTKAY-NEDECKY, B., KREMPLOVA, M., KRYSTOFOVA, O., KENSOVA, R., HYNEK D., BABULA, P., ZITKA, O., ZEMAN, L., ADAM, V., KIZEK, R. 2013. Effect of Different Doses of Organically Bound Selenium on Antioxidant Status and Levels of Metal Ions in Postpartum Sows. Int. J. Electrochem. Sci., 8: 6162 - 6179. Go to original source...
  5. HORKY, P., RUTTKAY-NEDECKÝ, B., NEJDL, L., RICHTERA, L., CERNEI, N., POHANKA, M., KOPEL, P., SKLADANKA, J., HLOUCALOVA, P., SLAMA, P., NEVRKLA, P., MLEJNKOVA, V., KLUSONOVA, I., KIZEK, R., ADAM, V. 2016a. Electrochemical Methods for Study of Influence of Selenium Nanoparticles on Antioxidant Status of Rats. Int. J. Electrochem Sci., 4: 2799 - 2824. DOI: 10.20964/110402799 Go to original source...
  6. HORKY, P., SOCHOR, J., SKLADANKA, J., KLUSONOVA, I., NEVRKLA, P. 2016c. Effect of Selenium, Vitamin E and C on Antioxidant Potential and Quality of Boar Ejaculate. Journal of Animal and Feed Sciences, 1: 29-36. DOI: 10.22358/jafs/65584/2016 Go to original source...
  7. HORKY, P. 2014. Influence of increased dietary selenium on glutathione peroxidase activity and glutathione concentration in erythrocytes of lactating sows. Ann. Anim. Sci., 4: 869 - 882. DOI: 10.2478/aoas-2014-0056 Go to original source...
  8. HORKY, P., TMEJOVA, K., KENOVA, R., CERNEI, N., KUDR, J., SAPAKOVA, E., RUTTKAY-NEDECKY, B., ADAM, V., KIZEK R. 2015. Effect of heat stress on the antioxidant activity of Boar ejaculate revealed by spectroscopic and electrochemical methods. Int. J. Electrochem. Sci., 10: 6610 - 6626. Go to original source...
  9. HORKY, P., SKLADANKA, J., NEVRKLA, P., SLAMA, P. 2016b. Effect of Diet Supplemented with Antioxidants (Selenium, Copper, vitamins E and C) on Antioxidant Status and Ejaculate Quality of Breeding Boars. Ann. Anim. Sci., (in press). Go to original source...
  10. HORKY, P. 2015. Effect of Selenium on Its Content in Milk and Performance of Dairy Cows in Ecological Farming. Potravinarstvo, 9: 324 - 329. DOI: 10.5219/492 Go to original source...
  11. HOSMANOVA, R. and DOUSA, M. 2007. HPLC determination of vitamin E in feed materials, compounded feeds and foods. Chemicke Listy, 101: 578 - 583.
  12. KLUSONOVA, I., HORKY, P., SKLADANKA, J., KOMINKOVA, M., HYNEK, D., ZITKA, O., SKARPA, P., KIZEK, R., ADAM, V. 2015. An Effect of Various Selenium Forms and Doses on Antioxidant Pathways at Clover (Trifolium pratense L.). Int. J. Electrochem. Sci., 10: 9975 - 9987. Go to original source...
  13. KOMINKOVA, M., HORKY, P., CERNEI, N., TMEJOVA, K., RUTTKAY-NEDECKY, B., GURAN, R., POHANKA, M., ZITKA, O., ADAM, V., KIZEK, R. 2015. Optimization of the Glutathione Detection by High Performance Liquid Chromatography with Electrochemical Detection in the Brain and Liver of Rats Fed with Taurine. Int. J. Electrochem. Sci., 10: 1716 - 1727. Go to original source...
  14. LEI, T., A. and MARSHALL W. D. 1995. Selenium speciation by high-performence liquid-chromatography with online decetion by atomic-absorption spectrometry. Applied Organometallic Chem., 9: 149 - 158. DOI: 10.1002/aoc.590090209 Go to original source...
  15. LIPENSKY, J., LUSTYKOVA, A., ČEŘOVSKY, J. 2010. Effect of season on boar sperm morphology. Journal of Central European Agriculture, 11: 465 - 468. DOI: 10.5513/JCEA01/11.4.866 Go to original source...
  16. LOVERCAM, K. W., STEVARD, K. R., LIN, X., FLOWERS, W. L. 2013. Effect of dietary selenium on boar sperm quality. Anim. Reprod. Sci., 138: 268 - 275. DOI: 10.1016/j.anireprosci.2013.02.016 Go to original source...
  17. MORAE, E. A., TORRES, C. A. A, GUIMARAES, J. D., DE CARVALHO, G. R., MURGAS, L. D. S., DA COSTA, E. P. 2010. Oil sources and levels of vitamin E supplementation in the diet on the quality of boar semen cooled at 17 and 5 degrees C. Revista Brasileira de Zootechnica - Brazilian Journal of Animal Science, 36: 1450 - 1456. DOI: 10.1590/S1516-35982010000700008 Go to original source...
  18. NEVRKLA, P., CECHOVA, M., HADAS, Z. 2014. Use of repopulation for optimizing sow reproductive performance and piglet loss. Acta Vet. Brno, 83: 321 - 325. DOI: 10.2754/avb201483040321 Go to original source...
  19. NEVRKLA, P., CECHOVA, M., HADAS, Z. 2012. Analysis of piglet losses in farrowing houses with different technologies. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 60: 267 - 274. DOI: 10.11118/actaun201260060267 Go to original source...
  20. RUDENKO, A. O. and KARTSOVA, L. A. 2010. Determination of Water-Soluble Vitamin B and Vitamin C in Combined Feed, Premixes, and Biologically Active Supplements by Reversed-Phase HPLC. J. Analytical Chem., 65: 71 - 76. DOI: 10.1134/S1061934810010132 Go to original source...

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY NC ND 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.