Acta Univ. Agric. Silvic. Mendelianae Brun. 2016, 64(2), 527-534 | DOI: 10.11118/actaun201664020527

Microbial Diversity of Betula Trees: Pollen, Catkins, Leaves Relatively of Flowering

Tetiana V. Shevtsova1, Jana Petrová2, Ján Brindza3, Kateryna G. Garkava1, Miroslava Kačániová2
1 Department of Biotechnology, Educational and Research Institute of Environmental Security, National Aviation University, Kosmonavta Komarova ave., 1, 03 058, Kyiv, Ukraine
2 Department of Microbiology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic
3 Institute of Biodiversity Conservation and Biosafety, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic

Quantitative microbiological analysis by dilution plating method of pollen and additional male and female catkins, leaves of Betula verrucosa Ehrh. and its two cultivars: 'Purpurea' and 'Youngii' relatively of flowering period of Betula has been realized with the aim to provide new knowledge of the microbiological quality of anemophilous pollen for processing and its further application. Qualitative microbiological analysis with MALDI-TOF MS Biotyper was used in the identification of aerobic, anaerobic mesophilic bacteria and coliforms. Mixed microbiota was determined, consisting of aerobic (4.68-4.89 log cfu/g) and anaerobic (3.30-3.48 log cfu/g) mesophilic bacteria, lactobacilli (0-3.48 log cfu/g), coliform bacteria (0-4.57 log cfu/g), fungi and yeast (3.78-3.95 log cfu/g) on the pollen grains, that indicates acceptable quality in comparison with the microbiological quality parameters for bee pollen. Pantoea agglomerans was found associated with pollen of Betula verrucosa Ehrh. Recommendations on the collection of anemophilous pollen were established.

Keywords: Betula verrucosa Ehrh., microbiota, pollen contamination, microbiological quality, flowering
Grants and funding:

This work has been supported by the Excellence Center for Agrobiodiversity Conservation and Benefit (ECACB) project implemented under the Operational Programme Research and Development financed by European Fund for Regional Development, ITMS 26220120015.

Prepublished online: May 4, 2016; Published: May 1, 2016  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Shevtsova, T.V., Petrová, J., Brindza, J., Garkava, K.G., & Kačániová, M. (2016). Microbial Diversity of Betula Trees: Pollen, Catkins, Leaves Relatively of Flowering. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 64(2), 527-534. doi: 10.11118/actaun201664020527
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. C4P S. R. O. ©2005. ALERGIA.SK. [Online]. Available at: www.alergia.sk.
    2. BOGDANOV, S. 2004. Quality and Standards of Pollen and Beeswax. Apiacta, 38: 334-341.
    3. BOGDANOV, S. 2005. Contaminants of bee products. Apidologie, 37: 1-18. DOI: 10.1051/apido:2005043 Go to original source...
    4. BRINDZA, J., GRÓF, J., BACIGÁLOVÁ, K., FERIANC, P., TÓTH, D. 2010. Pollen microbial colonization and food safety. Acta Chimica Slovaca, 3(1): 95-102.
    5. BROVARSKIJ, V., BRINDZA, J. 2010. Včelí obnôľkový peµ. Kyjev-Nitra: FOP I. S. Maidachenko.
    6. CAMPOS, M. G. R., BOGDANOV, S., BICUDO de ALMEIDA-MURADIAN, L., SZCZESNA, T., MANCEBO, Y., FRIGERIO, C., FERREIRA, F. 2008. Pollen composition and standardisation of analytical methods. Journal of Apicultural Research and Bee World, 47(2): 156-163. DOI: 10.3896/IBRA.1.47.2.12 Go to original source...
    7. DE LA FUENTE, L., BURDMAN, S. 2011. Pathogenic and beneficial plant-associated bacteria. Encyclopedia of Life Support Systems (EOLSS), edited by Lal, R. Oxford, UK: Eolss Publishers.
    8. DESPRÉS, V. R., HUFFMAN, J. A., BURROWS, S. M., HOOSE, C., SAFATOV, A. S., BURYAK, G., FRÖHLICH-NOWOISKY, J., ELBERT, W., ANDREAE, M. O., PÖSCHL, U., JAENICKE, R. 2012. Primary biological aerosol particles in the atmosphere: a review. Tellus, B, 64, 15598. [Online]. Available at: http://journals.co-action.net/index.php/tellusb/article/view/15598. [Accessed: 2012, February 22]. DOI: 10.3402/tellusb.v64i0.15598 Go to original source...
    9. DZIADZIO, L., BUSH, R. K. 2001. Assessment and control of fungal allergens. Current allergy and asthma reports, 1: 455-460. DOI: 10.1007/s11882-001-0033-3 Go to original source...
    10. ESTEVINHO, L. M., RODRIGUES, S., PEREIRA, A. P., FEÁS X. 2012. Portuguese bee pollen: palynological study, nutritional and microbiological evaluation. International Journal of Food Science and Technology, 47: 429-435. DOI: 10.1111/j.1365-2621.2011.02859.x Go to original source...
    11. FEÁS, X., PILAR VÁZQUEZ-TATO, M., ESTEVINHO, L., SEIJAS, J. A., IGLESIAS, A. 2012. Organic Bee Pollen: Botanical Origin, Nutritional Value, Bioactive Compounds, Antioxidant Activity and Microbiological Quality. Molecules, 17: 8359-8377. DOI: 10.3390/molecules17078359 Go to original source...
    12. GONZÁLEZ, G., HINOJO, M. J., MATEO, R., MEDINA, A., JIMÉNEZ, M. 2005. Occurrence of mycotoxin producing fungi in bee pollen. International Journal of Food Microbiology, 105(1): 1-9. DOI: 10.1016/j.ijfoodmicro.2005.05.001 Go to original source...
    13. HANI, B., DALILA, B., SALIHA, D., DAOUD, H., MOULOUD, G., SEDDIK, K. 2012. Microbiological sanitary aspects of pollen. Advances in Environmental Biology, 6(4): 1415-1420.
    14. HEYDENREICH, B., BELLINGHAUSEN, I., KÖNIG, B., BECKER, W.-M., GRABBE, S., PETERSEN, A., SALOGA, J. 2011. Gram-positive bacteria on grass pollen exhibit adjuvant activity inducing inflammatory T cell responses. Clinical & Experimental Allergy, 42: 76-84. DOI: 10.1111/j.1365-2222.2011.03888.x Go to original source...
    15. KAČÁNIOVÁ, M., FIKSELOVÁ, M. 2007. Mycological flora on tree fruits, crust, leaves and pollen Sorbus domestica L. Annals of Agricultural and Environmental Medicine, 14: 229-232.
    16. KAČÁNIOVÁ, M., JURÁČEK, M., CHLEBO, R., KŇAZOVICKÁ, V., KADASI-HORÁKOVÁ, M., KUNOVÁ, S., LEJKOVÁ, J., HA©ČÍK, P., MAREČEK, J., ©IMKO, M. 2011. Mycobiota and mycotoxins in bee pollen collected from different areas of Slovakia. Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants and Agricultural Wastes, 46(7): 623-629. DOI: 10.1080/03601234.2011.589322 Go to original source...
    17. KAČÁNIOVÁ, M., PETROVÁ, J., HLEBA, L., SHEVTSOVA, T., KLUZ, M., RO®EK, P., VUKOVIČ, N. 2014. Microscopic filamentous fungi occurrence in plant pollen from nontraditional plant species. Journal of Microbiology, Biotechnology and Food Sciences, 3(6): 522-524. Available at: http://www.jmbfs.org/issue/june-july-2014-vol-3-no-6/jmbfs_xxk4_kacaniova/?issue_id=3355&article_id=19. [Accessed: 2014, June 1]
    18. KINI, G. D., PATEL, K., PARRIS, A. R., TANG J. S. 2010. An unusual presentation of endocarditis caused by Staphylococcus warneri. Open Microbiol. J., 4: 103-105. DOI: 10.2174/1874285801004010103 Go to original source...
    19. KIRK, P. M., CANNON, P. F., MINTER, D. W. et al. 2008. Dictionary of the Fungi. 10th edition. Wallingford, UK: CABI Publishing.
    20. LARKIN, M., KULAKOV, L., ALLEN, C. 2010. Genomes and plasmids in Rhodococcus. Biology of Rhodococcus, 16: 73-90. DOI: 10.1007/978-3-642-12937-7_3 Go to original source...
    21. MADMONY, A., CHERNIN, L., PLEBAN, S., PELEG, E., RIOV, J. 2005. Enterobacter cloacae, an obligatory endophyte of pollen grains of Mediterranean pines. Folia Microbiol., 50(3): 209-216. DOI: 10.1007/BF02931568 Go to original source...
    22. MITTAG, D., VARESE, N., SCHOLZEN, A., MANSELL, A., BARKER, G., RICE, G., ROLLAND, J. M., O'HEHIR, R. E. 2013. TLR ligands of ryegrass pollen microbial contaminants enhance Th1 and Th2 responses and decrease induction of Foxp3(hi) regulatory T cells. Eur. J. Immunol., 43(3): 723-733. DOI: 10.1002/eji.201242747 Go to original source...
    23. MUKAMOLOVA, G. V., TURAPOV, O. A., KAZARIAN, K., TELKOV, M., KAPRELYANTS, A. S., KELL, D. B., YOUNG, M. 2002. The rpf gene of Micrococcus luteus encodes an essential secreted growth factor. Molecular Microbiology, 46(3): 611-621. DOI: 10.1046/j.1365-2958.2002.03183.x Go to original source...
    24. NÔ®KOVÁ, J., KAČÁNIOVÁ, M. and FATRCOVÁ-©RAMKOVÁ, K. 2014. Evaluation of bee pollen quality [in Slovak: Hodnotenie kvality obnôľkového peµu]. Nitra: Vydavateµstvo SPU v Nitre.
    25. RODRÍGUEZ-CARRASCO, Y., FONT, G., MAŇES, J., BERRADA, H. 2013. Determination of mycotoxins in bee pollen by gas chromatography - tandem mass spectrometry. J. Agric. Food Chem., 61(8): 1999-2005. DOI: 10.1021/jf400256f Go to original source...
    26. SHEVTSOVA, T., GARKAVA, E., BRINDZA, J., BRJUZGINA, T., GROZA, V. 2013. Protein-lipid composition of silver birch (Betula verrucosa) pollen and its antioxidant activity depending on habitat. Visnyk of Dnipropetrovsk University. Biology, ecology, 21(2): 105-112. DOI: 10.15421/011318 Go to original source...
    27. SHINHA, S., CHATTOPADHYAY, P., PAN, I., CHATTERJEE, S., CHANDA, P., BANDYOPADHYAY, D., DAS, K., SEN, S. K. 2009. Microbial transformation of xenobiotics for environmental bioremediation. African Journal of Biotechnology, 8(22): 6016-6027. Go to original source...
    28. ¦PIEWAK, R., KRYSIŃSKA-TRACZYK, E., SITKOWSKA, J., DUTKIEWICZ, J. 1996. Microflora of allergenic pollens - a preliminary study. Annals of Agricultural and Environmental Medicine, 3: 127-130.
    29. ¦PIEWAK, R., SKÓRSKA, C., PRAŻMO, Z., SITKOWSKA, J., DUTKIEWICZ, J. 1996. Bacterial endotoxin associated with pollen as a potential factor aggravating pollinosis. Ann. Agric. Environ. Med., 3(1): 57-59.
    30. ©USTROVÁ, M. 2009. Seasonal allergies [in Slovak: Sezónne alergie]. In: ZZZ.sk. [Online]. Available at: http://www.zzz.sk/?clanok=6237. [Accessed: 2009, May 1].
    31. TÓTH, E. M., BORSODI, A. K., FELFÖLDI, T. et al. 2013. Practical Microbiology: based on the Hungarian practical notes entitled "Mikrobiológiai Laboratóriumi Gyakorlatok". Budapest: Eötvös Loránd University.
    32. WARHURST, A. M., FEWSON, C. A. 1994. Biotransformations catalyzed by the genus Rhodococcus. Critical reviews in biotechnology, 14(1): 29-73. DOI: 10.3109/07388559409079833 Go to original source...
    33. YOON, J.-H., KANG, S. S., CHO, Y. G., LEE, S. T., KHO, Y. H., KIM, C. J., PARK, Y. H. 2000. Rhodococcus pyridinivorans sp. nov., a pyridine-degrading bacterium. Int. J. Sys. Evol. Microbiol., 50: 2173-2180. DOI: 10.1099/00207713-50-6-2173 Go to original source...
    34. ZHAO, J. S., FOURNIER, D., THIBOUTOT, S., AMPLEMAN, G. and HAWARI, J. 2004. Biodegradation and bioremediation of explosives. Applied Bioremediation and Phytoremediation, Soil Biology, 1: 55-80. DOI: 10.1007/978-3-662-05794-0_4 Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY NC ND 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content