Acta Univ. Agric. Silvic. Mendelianae Brun. 2016, 64(2), 411-416 | DOI: 10.11118/actaun201664020411

Superoxide Dismutase in Spring Barley Caryopses

Natálie Březinová Belcredi1, Kateřina Vaculová2
1 Department of Crop Science, Breeding and Plant Medicine, Faculty of AgriSciences, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic
2 Agrotest fyto, Ltd, Havlíčkova 2787/121, 767 01 Kroměříž, Czech Republic

Superoxide dismutase (SOD) activity was determined in caryopses of spring barley grown in field trials in 2004-2006. A total set under study included five malting varieties with hulled grain, three waxy hull-less and hulled varieties (of US origin), seven lines formed by crossing of the above given varieties and four hull-less lines of Czech origin. SOD activity was determined by a modified method using a Ransod diagnostic kit (RANDOX). The method employs xanthine and xanthine oxidase to generate superoxide radicals which react with 2-(4-iodophenyl)-3-(4-nitrophenol)-5-phenyltetrazolium chloride (INT) to form a red formazan dye. Statistically significantly higher activity was measured in the variety Nordus (131 U.g-1 d.m.) and line ME1 (128 U.g-1 d.m.) compared to the other varieties/lines (66-111 U.g-1 d.m.). The line ME1 had significantly higher SOD activity in grain versus its parental varieties Kompakt (83 U.g-1 d.m.) and Krona (78 U.g-1 d.m.). The results of this study proved the availability of varieties/lines with a higher SOD content, the antioxidant effect of SOD can improve quality of beer and food made from barley.

Keywords: antioxidant, enzyme, superoxide dismutase, SOD, hull-less, spring barley, Ransod
Grants and funding:

The study was supported by The Ministry of Agriculture of the Czech Republic, Project No. QI 91B095 and Project No. MSM2532885901.

Prepublished online: May 4, 2016; Published: May 1, 2016  Show citation

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Březinová Belcredi, N., & Vaculová, K. (2016). Superoxide Dismutase in Spring Barley Caryopses. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 64(2), 411-416. doi: 10.11118/actaun201664020411
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