Acta Univ. Agric. Silvic. Mendelianae Brun. 2016, 64(2), 395-399 | DOI: 10.11118/actaun201664020395

Effect of LDL Addition Into Selected Bull Sperm Diluters on Resistance of Spermatozoa Against Cold Shock

Jan Beran1, Ondřej Šimoník2, Radko Rajmon2, Luděk Stádník1, Martina Doležalová1, Adéla Krejcárková2, Jaromír Ducháček1, Jiří Šichtař2
1 Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 21 Praha 6-Suchdol, Czech Republic
2 Department of Veterinary Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 21 Praha 6-Suchdol, Czech Republic

The aim of work was to determine the effect of LDL cholesterol addition into selected diluters on the resistance of spermatozoa against cold shock and on their short-term survivability during cold test. The hypothesis was that the addition of LDL cholesterol will positively affects sperm resistance to cold shock and ensures a higher survivability of spermatozoa during short-term cold survival test. Four bulls of different breeds and ages, from the same sire insemination center were used. A total of eight semen collections were processed. Each ejaculate was divided into 6 portions (3 controls and 3 samples). Three commercially produced diluters, AndroMed®, Bioxcell®, and Triladyl® were used, each in standard and LDL enriched variants. In the case of AndroMed® or Bioxcell®, 6% of LDL was simply added. In Triladyl®, 10% of LDL replaced the standard egg yolk component. Spermatozoa resistance to cold shock was evaluated by the percentage of live sperm using Eosin-Nigrosine staining. The results showed the influence of bull individuality as an important factor. It is possible to recommend Bioxcell® with addition of LDL cholesterol in 6% concentration, which survivability was 69.17% at the beginning of the test, and 52.94% after 2 hours of incubation.

Keywords: AndroMed, Bioxcell, bull semen, LDL, Triladyl, sperm survivability
Grants and funding:

The paper was supported by "S" grant of MŠMT ČR and NAZV [No. QJ1210109].

Prepublished online: May 4, 2016; Published: May 1, 2016  Show citation

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Beran, J., Šimoník, O., Rajmon, R., Stádník, L., Doležalová, M., Krejcárková, A., Ducháček, J., & Šichtař, J. (2016). Effect of LDL Addition Into Selected Bull Sperm Diluters on Resistance of Spermatozoa Against Cold Shock. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 64(2), 395-399. doi: 10.11118/actaun201664020395
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