Acta Univ. Agric. Silvic. Mendelianae Brun. 2016, 64(1), 165-168 | DOI: 10.11118/actaun201664010165
Mycoplasma bovis Was not Detected in Milk from Dairy Cattle in the Czech Republic
- Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic
The aim of this study was to screen selected Czech dairy cattle farms for presence of Mycoplasma bovis. Between 2012 and 2014, 160 individual samples of cow milk, 60 colostrum samples, and 105 bulk-tank milk samples collected on 21 dairy farms were analyzed in our laboratory. DNA was extracted from samples and tested by quantitative PCR assay for M. bovis. All analyzed samples were negative. Results support long-term trend of very low prevalence of M. bovis in the Czech Republic.
Keywords: Mycoplasma bovis, respiratory disease, mastitis, cattle, qPCR
Grants and funding:
This survey was funded by Internal Grant Agency of Mendel University in Brno [IP 13/2014] and Ministry of Agriculture of Czech Republic [QJ1210301]. Work on survey was realized in CEITEC - Central European Institute of Technology - using research infrastructure supported by the project CZ.1.05/1.1.00/02.0068 financed from European Regional Development Fund.
Prepublished online: February 29, 2016; Published: April 1, 2016 Show citation
ACS | AIP | APA | ASA | Harvard | Chicago | IEEE | ISO690 | MLA | NLM | Turabian | Vancouver |
References
- BLOWEY, R. W., EDMONDSON, P. 2010. Mastitis control in dairy herds. 2nd edition. London, UK: CAB International.
Go to original source...
- BYRNE, W., MARKEY, B., MCCORMACK, R., EGAN, J., BALL, H. and SACHSE, K. 2005. Persistence of Mycoplasma bovis infection in the mammary glands of lactating cows inoculated experimentally. Vet. Rec., 156: 767-771. DOI: 10.1136/vr.156.24.767
Go to original source...
- CHAZEL, M., TARDY, F., LE GRAND, D., CALAVAS, D. and POUMARAT, F. 2010. Mycoplasmoses of ruminants in France: recent data from the national surveillance network. BMC Vet. Res., 6: 32-39. DOI: 10.1186/1746-6148-6-32
Go to original source...
- GHADERSOHI, A, COELEN, R. J. and HIRST, R. G. 1997. Development of a specific DNA probe and PCR for the detection of Mycoplasma bovis. Vet. Microbiol., 56: 87-98. DOI: 10.1016/S0378-1135(96)01343-0
Go to original source...
- GONZALEZ, R. N. and WILSON, D. J. 2003. Mycoplasmal mastitis in dairy herds. Vet. Clin. North. Am. Food. Anim. Pract., 19: 199-221. DOI: 10.1016/S0749-0720(02)00076-2
Go to original source...
- JOZEFOVÁ, J., AYLING, R. D. and ZENDULKOVÁ, D. 2014. Report on investigation into the Mycoplasma species present in some clinically sick cattle in the Czech Republic. Med. Weter., 70: 413-416.
- JURMANOVÁ, K., HÁJKOVÁ, M., KREJČÍ, J. and ČERNÁ, J. 1975. The participation of mycoplasmas in the respiratory diseases of calves in large-capacity calf houses in the Czechoslovak Socialist Republic. Vet. Med. Praha, 20: 583-593.
- MCAULIFFE, L., KOKOTOVIC, B., AYLING, R. D. and NICHOLAS, R. A. J. 2004. Molecular epidemiological analysis of Mycoplasma bovis isolates from the United Kingdom shows two genetically distinct clusters. J. Clin. Microbiol., 42: 4556-4565. DOI: 10.1128/JCM.42.10.4556-4565.2004
Go to original source...
- MAUNSELL, F. P., WOOLUMS, A. R., FRANCOZ, D., ROSENBUSCH, R. F., STEP, D. L., WILSON, D. J. and JANZEN, E. D. 2011. Mycoplasma bovis infections in cattle. J. Vet. Intern. Med., 25: 772-783. DOI: 10.1111/j.1939-1676.2011.0750.x
Go to original source...
- PFUTZNER, H., SCHERW, A. B. and TRUBNER, S. 1983. Sensitivity of Mycoplasma bovis to disinfection agents applied to the udder area. Arch. Exp. Veterinarmed., 37: 485-489.
- PFUTZNER, H. and SACHSE, K. 1996. Mycoplasma bovis as an agent of mastitis, pneumonia, arthritis and genital disorders in cattle. Rev. Sci. Tech., 15: 1477-1494. DOI: 10.20506/rst.15.4.987
Go to original source...
- PUNYAPORNWITHAYA, V., FOX, L. K., HANCOCK, D. D., GAY, J. M. and ALLDREDGE, J. R. 2010. Association between an outbreak strain causing Mycoplasma bovis mastitis and its asymptomatic carriage in the herd: a case study from Idaho, USA. Prev. Vet. Med., 93: 66-70. DOI: 10.1016/j.prevetmed.2009.08.008
Go to original source...
- SACHSE, K., PFUTZNER, H., HOTZEL, H., DEMUTH, B., HELLER, M. and BERTHOLD, E. 1993. Comparison of various diagnostic methods for the detection of Mycoplasma bovis. Rev. Sci. Tech. Off. Int. Epiz., 12: 571-580. DOI: 10.20506/rst.12.2.701
Go to original source...
- SACHSE, K., SALAM, H. S. H., DILLER, R., SCHUBERT, E., HOFFMANN, B. and HOTZEL, H. 2010. Use of a novel real-time PCR technique to monitor and quantitate Mycoplasma bovis infection in cattle herds with mastitis and respiratory disease. Vet. J., 186: 299-303. DOI: 10.1016/j.tvjl.2009.10.008
Go to original source...
- STIPKOVITS, L., ROSENGARTEN, R. and FREY, J. 1999. Mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiology and molecular genetics. Brussels: European Commission.
- ZENDULKOVÁ, D., HAAS, D., ROSENBERGOVÁ, K., POSPÍŠIL, Z., LANY, P. and BURDOVÁ, E. 2007. Bacterial profile of bovine respiratory disease in young calves. Suppl. Rev. Rom. Med. Vet., 2(17): 64.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY NC ND 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.