Acta Univ. Agric. Silvic. Mendelianae Brun. 2015, 63(4), 1111-1119 | DOI: 10.11118/actaun201563041111

Sensitivity of Colletotrichum acutatum Isolates to Selected Fungicides

Barbora Jílková1, Jana Víchová1, Radovan Pokorný1, Karel Vejražka2
1 Department of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic
2 Agricultural Research, Ltd. Troubsko, Zahradní 1, 664 41 Troubsko, Czech Republic

Laboratory tests of six isolates of the pathogen Colletotrichum acutatum from different host plants demonstrated the varying sensitivity of pathogen with regard to mycelium growth and conidial germination after treatment with seven fungicides containing various active ingredients. None of the evaluated isolates was tolerant to the selected active ingredients in the fungicides. In tests of mycelium growth sensitivity, isolates from lupin and strawberry were most frequently identified as the most sensitive of all evaluated fungicides. The safflower isolate, on the other hand, most frequently exhibited the lowest reaction to fungicides. Differences in conidial germination of individual isolates were not detected in fungicides with the active ingredients dithianon, folpet and mancozeb, for which inhibition reached 100% in almost all isolates. The most significant differences in sensitivity among individual isolates were recorded in fungicides with the active ingredients azoxystrobin and metiram. In the case of the fungicide with active ingredient azoxystrobin, the highest inhibitory effect was achieved in the safflower isolate and the lowest in the white lupin isolate. After treatment with the fungicide with active ingredient metiram, the lowest germination rate was recorded in isolates from safflower and strawberry and the highest in isolates from hypericum and lupin.

Keywords: azoxystrobin, captan, dithianon, folpet, mancozeb, metiram, thiram, fungal pathogen
Grants and funding:

This study was supported by NAZV MZe No. QH81029: "Innovation protection of safflower (Carthamus tinctorius L.) against important fungal pathogens".

Prepublished online: September 2, 2015; Published: September 1, 2015  Show citation

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Jílková, B., Víchová, J., Pokorný, R., & Vejražka, K. (2015). Sensitivity of Colletotrichum acutatum Isolates to Selected Fungicides. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 63(4), 1111-1119. doi: 10.11118/actaun201563041111
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    References

    1. AGOSTINI, J. P., TIMMER, L. W., MITCHELL, D. J. 1992. Morphological and pathological characteristics of strains of Colletotrichum gloeosporioides from citrus. Phytopathology, 82(11): 1377-1382. DOI: 10.1094/Phyto-82-1377 Go to original source...
    2. FREEMAN, S., HOROWITZ, S., SHARON, A. 2001. Pathogenic and nonpathogenic lifestyles in Colletotrichum acutatum from strawberry and other plants. Phytopathology, 91(10): 986-992. DOI: 10.1094/PHYTO.2001.91.10.986 Go to original source...
    3. GOES, A., KIMATI, H. 1998. Colletotrichum acutatum, the causal agent of postbloom fruit drop: resistant or insensitive to benomyl? Summa Phytopathologica, 24(3/4): 246-253.
    4. GOES, A., GARRIDO, R. B. O., REIS, R. F., BALDASSARI, R. B., SOARES, M. A. 2008. Evaluation of fungicide applications to sweet orange at different flowering stages for control of postbloom fruit drop caused by Colletotrichum acutatum. Crop Protection, 27 (1): 71-76. DOI: 10.1016/j.cropro.2007.04.007 Go to original source...
    5. HOWARD, C. M., MAAS, J. L., CHANDLER, C. K., ALBREGTS, E. E. 1992. Anthracnose of strawberry caused by the Colletotrichum complex in Florida. Plant Disease, 76(10): 976-981. DOI: 10.1094/PD-76-0976 Go to original source...
    6. HWANG, S.-Y., KIM, H.-R., KIM, J.-H., PARK, J.-H., LEE, S.-B., CHEONG, S-R., KIM, H.-T. 2010. Sensitivity of Colletotrichum spp. isolated from Grapes in Korea to carbendazim and the mixture of carbendazim plus diethofencarb. Plant Pathology Journal, 26(1): 49-56. DOI: 10.5423/PPJ.2010.26.1.049 Go to original source...
    7. KIM, S. G., KIM, Y.-H., KIM, H.-T., KIM, Y. H. 2008. Effect of delayed inoculation after wounding on the development of anthracnose disease caused by Colletotrichum acutatum on chili pepper fruit. Plant Pathology Journal, 24(4): 392-399. DOI: 10.5423/PPJ.2008.24.4.392 Go to original source...
    8. KIM, Y.-S., MIN, J. Y., KANG, B. K., BACH, N. V., CHOI, W. B., PARK, E. W., KIM, H. T. 2007. Analyses of the less benzimidazole-sensitivity of the isolates of Colletotrichum spp. causing the anthracnose in pepper and strawberry. Plant Pathology Journal, 23(3): 187-192. DOI: 10.5423/PPJ.2007.23.3.187 Go to original source...
    9. KLOUTVOROVÁ, J., KUPKOVÁ, J. 2009. Laboratorní testy citlivosti houby Colletotrichum acutatum k fungicidům. Vědecké práce ovocnářské, 21: 47-52.
    10. MACHOWICZ-STEFANIAK, Z., ZALEWSKA, E. 2011. Occurrence of Colletotrichum dematium on selected herbs species and preparations inhibiting pathogen's growth and development in vitro. Ecological Chemistry and Engineering S, 18(4): 465-478.
    11. MACKENZIE, S. J., PERES, N. A. 2012. Use of leaf wetness and temperature to time fungicide applications to control anthracnose fruit rot of strawberry in Florida. Plant Disease, 96(4): 522-528. DOI: 10.1094/PDIS-03-11-0181 Go to original source...
    12. MARI, M., GUIDARELLI, M., MARTINI, C., SPADONI, A. 2012. First report of Colletotrichum acutatum causing bitter rot on apple in Italy. Plant Disease, 96(1): 144. DOI: 10.1094/PDIS-06-11-0483 Go to original source...
    13. NOVOTNÁ, K., STAŇKOVÁ, B., VÍCHOVÁ, J.,VEJRAŽKA, K., POKORNÝ, R. 2011. Účinnost dvousložkových fungicidů na vybrané izoláty Colletotrichum acutatum. Úroda, 59(12): 243-246.
    14. NOVOTNÝ, D., KŘÍŽKOVÁ-KUDLÍKOVÁ, I., KRÁTKÁ, J., SALAVA, J. 2006. The presence of Colletotrichum acutatum in the Czech Republic. In: Sborník abstraktů "XVII. česká a slovenská konference o ochraně rostlin", Česká zemědělská univerzita v Praze, 12.-14. září. Praha: Česká zemědělská univerzita, 252.
    15. PERES, N. A., MACKENZIE, S. J., PEEVER, T. L., TIMMER, L. W. 2008. Postbloom fruit drop of citrus and Key lime anthracnose are caused by distinct phylogenetic lineages of Colleotrichum acutatum. Phytopathology, 98(3): 345-352. DOI: 10.1094/PHYTO-98-3-0345 Go to original source...
    16. PERES, N. A., SEIJO, T. E., TURECHEK, W. W. 2010. Pre- and post-inoculation activity of a protectant and a systemic fungicide for control of anthracnose fruit rot of strawberry under different wetness durations. Crop Protection, 29: 1105-1110. DOI: 10.1016/j.cropro.2010.05.010 Go to original source...
    17. PERES, N. A., SOUZA, N. L., PEEVERT, T. L., TIMMER, L. W. 2004. Benomyl sensitivity of isolates of Colletotrichum acutatum and C. gloeosporioides from citrus. Plant Disease, 88(2): 125-130. DOI: 10.1094/PDIS.2004.88.2.125 Go to original source...
    18. SHUKLA, R. S., KHALIQ, A., ALAM, M. 2010. Chemical control of blossom blight disease of sarpagandha caused by Colletotrichum capsici. African Journal of Biotechnology, 9(38): 6397-6400.
    19. SREENIVASAPRASAD, S., TALHINHAS, P. 2005. Genotypic and phenotypic diversity in Colletotrichum acutatum, a cosmopolitan pathogen causing anthracnose on a wide range of hosts. Molecular Plant Pathology, 6(4): 361-378. DOI: 10.1111/j.1364-3703.2005.00291.x Go to original source...
    20. STAŇKOVÁ, B., VÍCHOVÁ, J., POKORNÝ, R. 2011. Virulence of Colletotrichum acutatum isolates to several host plants. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 59(3): 161-169. DOI: 10.11118/actaun201159030161 Go to original source...
    21. VÍCHOVÁ, J., VEJRAŽKA, K., CHOLASTOVÁ, T., POKORNÝ, R., HRUDOVÁ, E. 2011. Colletotrichum simmondsii causing anthracnose on safflower in the Czech Republic. Plant Disease, 95(1): 79. DOI: 10.1094/PDIS-08-10-0614 Go to original source...
    22. VÍCHOVÁ, J., STAŇKOVÁ, B., POKORNÝ, R. 2012. First report of Colletotrichum acutatum on tomato and apple fruits in the Czech Republic. Plant Disease, 96(5): 769-770. DOI: 10.1094/PDIS-10-11-0849-PDN Go to original source...
    23. VÍCHOVÁ, J., STAŇKOVÁ, B., POKORNÝ, R. 2013. First report of Colletotrichum acutatum sensu lato causing anthracnose on gooseberry fruits in the Czech Republic. Plant Disease, 97(9): 1249. DOI: 10.1094/PDIS-01-13-0080-PDN Go to original source...
    24. WHARTON, P. S., DIÉGUEZ-URIBEONDO, J. 2004. The biology of Colletotrichum acutatum. Anales del Jardín Botánico de Madrid, 61(1): 3-22. DOI: 10.3989/ajbm.2004.v61.i1.61 Go to original source...
    25. WONG, F. P., CERDA, K. A., DE LA HERNANDEZ-MARTINEZ, R. MIDLAND, S. L. 2008. Detection and characterization of benzimidazole resistance in California populations of Colletotrichum cereale. Plant Disease, 92(2): 239-246. DOI: 10.1094/PDIS-92-2-0239 Go to original source...
    26. XU, X. F., LIN, T., YUAN, S. K., DAI, D. J., SHI, H. J., ZHANG, C. Q., WANG, H. D. 2014. Characterization of baseline sensitivity and resistance risk of Colletotrichum gloeosporioides complex isolates from strawberry and grape to two demethylation-inhibitor fungicides, prochloraz and tebuconazole. Australasian Plant Pathology, 43(6): 605-613. DOI: 10.1007/s13313-014-0321-8 Go to original source...
    27. YOUNG, J. R. TOMASO-PETERSON, M. CERDA, K., DE LA WONG, F. P. 2010. Two mutations in beta -tubulin 2 gene associated with thiophanate-methyl resistance in Colletotrichum cereale isolates from creeping bentgrass in Mississippi and Alabama. Plant Disease, 94(2): 207-212. DOI: 10.1094/PDIS-94-2-0207 Go to original source...
    28. ZHANG, L. H., LI, M., GAO, Z. Y., ZHANG, Z. K., YANG, F. Z., XIE, Y. X., HU, M. J., YANG, Y. 2013. Screening and cross-resistance analysis of alternative fungicides against carbendazim-resistant Colletotrichum gloeosporioides Penz. from mango (Mangifera indica L.). Acta Horticulturae, 992: 415-421. DOI: 10.17660/ActaHortic.2013.992.52 Go to original source...

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