Acta Univ. Agric. Silvic. Mendelianae Brun. 2012, 60(8), 229-238 | DOI: 10.11118/actaun201260080229

Response of grapevine leaves to Plasmopara viticola infection by means of measurement of reflectance and fluorescence signals

David ©ebela1, Julie Olejníčková2, Anita ®upčanová3, Radek Sotolář4
1 Laboratoř ekologické fyziologie rostlin, Centrum výzkumu globální změny AV ČR, v.v.i., Bělidla 986/4a, 603 00 Brno, Ústav fyziky a biofyziky, Přírodovědecká fakulta, Jihočeská univerzita v Českých Budějovicích, Braniąovská 31, 370 05 České Budějovice, Czech Republic
2 Laboratoř ekologické fyziologie rostlin, Centrum výzkumu globální změny AV ČR, v.v.i., Bělidla 986/4a, 603 00 Brno, Czech Republic
3 Ústav fyziky a biofyziky, Přírodovědecká fakulta, Jihočeská univerzita v Českých Budějovicích, Braniąovská 31, 370 05 České Budějovice, Czech Republic
4 Ústav vinohradnictví a vinařství, Mendelova univerzita v Brně, Valtická 337, 691 44 Lednice, Czech Republic

Response of grapevine leaf tissue naturally infected by Plasmopara viticola in field was measured by means of chlorophyll fluorescence and reflectance signals. Three susceptible grapevine varieties (Cabernet Sauvignon, Pinot Blanc and Pinot Gris) were used in this study. Since the infection impairs photosynthetic activity, distribution of FV/FM parameter (maximum quantum yield of Photosystem II) over the leaf was effective to discriminate healthy and naturally infected leaf tissue. FV/FM was reduced ~ 25% in all infected leaf parts. Infected leaf spots expressed significantly altered chlorophyll fluorescence induction kinetics expressing much slower electron transport rate both on donor and acceptor site of PSII. Vegetation reflectance indices followed the variations in pigment content after the fungal infection. R750/R700 (R2 = 0.877) and CRI (carotenoid reflectance index; R2 = 0.735) were the most potent to follow changes in chlorophylls and carotenoids contents, respectively. Infected leaf tissue exhibited decrease in chlorophyll a (~50 %) as well as carotenoids (~70%). We conclude that combination of chlorophyll fluorescence and reflectance measurements can be used as an effective non-invasive tool for an early detection of Plasmopara viticola in field as well as for estimation of the level of infection.

Keywords: Plasmopara viticola, downy mildew, grapevine, leaf tissue, susceptible varieties, chlorophyll fluorescence imaging, reflectance
Grants and funding:

This study was funded by the Grant Agency of the Czech Republic, grant No. 525/09/0365, and by CzechGlobe - Centre for Global Climate Change Impacts Studies, Reg. No. CZ.1.05/1.1.00/02.0073.

Received: September 13, 2012; Published: March 30, 2013  Show citation

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©ebela, D., Olejníčková, J., ®upčanová, A., & Sotolář, R. (2012). Response of grapevine leaves to Plasmopara viticola infection by means of measurement of reflectance and fluorescence signals. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 60(8), 229-238. doi: 10.11118/actaun201260080229
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    References

    1. BARBAGALLO, R. P., OXBOROUGH, K., PALLET, K. E., BAKER, N. R., 2003: Rapid, noninvasive screening for pertubations of metabolism and plant growth using chlorophyll fluorescence imaging. Plant Physiol., 132: 485-493. DOI: 10.1104/pp.102.018093 Go to original source...
    2. BELLASIO, CH., OLEJNÍČKOVÁ, J., TESAŘ, R., ©EBELA, D., NEDBAL, L., 2012: Computer reconstruction of plant growth and chlorophyll fluorescence emission in 3 spatial dimensions. Sensors, 12(1): 1052-1071. DOI: 10.3390/s120101052 Go to original source...
    3. BJORN, L. O., PAPAGEORGIOU, G. C., BLANKENSHIP, R. E., GOVINDJEE, 2009: A viewpoint: why chlorophyll a? Photosynth. Res., 99: 89-98. DOI: 10.1007/s11120-008-9395-x Go to original source...
    4. BLACKBURN, G. A., 2007: Hyperspectral remote sensing of plant pigments. J. Exp. Bot., 58(4): 855-867. DOI: 10.1093/jxb/erl123 Go to original source...
    5. BLACKBURN, G. A., FERWERDA, J. G, 2008: Retrieval of chlorophyll concentration from leaf reflectance spectra using wavelet analysis. Remote Sens.Env., 112: 1614-1632. DOI: 10.1016/j.rse.2007.08.005 Go to original source...
    6. CSÉFALVAY, L., DI GASPERO, G., MATOU©, K., BELLIN, D., RUPERTI, B., OLEJNÍČKOVÁ, J., 2009: Pre-symptotic detection of Plasmopara viticola infection in grapevine leaves using chlorophyll fluorescence imaging. Eur. J. Plant Pathol., 125: 291-302. DOI: 10.1007/s10658-009-9482-7 Go to original source...
    7. DATT, B., 1999: Visible/near infrared reflectance and chlorophyll content in Eucalyptus leaves. Int. J. Remote Sens., 20(14): 2741-2759. DOI: 10.1080/014311699211778 Go to original source...
    8. DAVIES, M. K., 2004: Plant pigments and their manipulation. Annual plant reviews. Blackwell Publishing, vol. 14, Oxford-Boca Raton, 2004.
    9. DIEZ-NAVAJAS, A. M, WIEDEMANN-MERDINOGLU, S., GREIF, C., MERDINOGLU, D., 2008: Nonhost versus Host resistance to the grapevine Downy Mildew, Plasmopara viticola, studied at tissue level. Phytopatology, 98(7): 776-780. DOI: 10.1094/PHYTO-98-7-0776 Go to original source...
    10. FAROUK, S., OSMAN, M. A., 2011: The effect of plant defense elicitors on common bean (Phaseolus vulgaris L.) growth and yield in absence or prsence of spider mite (Tetranychus urticae Koch) infestation. J. Stress Physiol. Biochem., 7(3): 5-22.
    11. FILELLA, I., PORCAR-CASTELL, A., MUNNE-BOSCH, S., BACK, J., GARBULSKY, M. F., PENUELAS, J., 2009: PRI assessment of long-term changes in carotenoid/chlorophyll ratio and short-term changes in de-epoxidation state of the xanthophyll cycle. Int. J. Remote Sens., 30: 4443-4455. DOI: 10.1080/01431160802575661 Go to original source...
    12. FUNAYAMA, S., SONOIKE, K., TERASHIMA, I., 1997: Photosynthetic properties of leaves of Eupatorium makinoi infected by a geminivirus. Photosynth. Res., 53: 253-261. DOI: 10.1023/A:1005884007183 Go to original source...
    13. GITELSON, A., 1992: The peak near 700nm on radiance spectra of algae and water: relationship of its magnitude and position with chlorophyll concentration. Int. J. Remote Sens., 17 (13): 3367-3373. DOI: 10.1080/01431169208904125 Go to original source...
    14. GITELSON, A., MERZLYAK, M. N., 1994: Spectral reflectance changes associated with autumn senescence of Aesculus hippocastanum L. and Acer platanoides L. leaves. Spectral features and relation to chlorophyll estimation. J.Plant Physiol., 143: 286-292. DOI: 10.1016/S0176-1617(11)81633-0 Go to original source...
    15. GITELSON, A. A., MERZLYAK, M. N., YURI, G., 1996: Novel algorithms for remote sensing of chlorophyll content in higher plant leaves. Geoscience and Remote Sens. Symposium, 4: 2355-2357. Go to original source...
    16. GITELSON, A. A., MERZLYAK, M. N., CHIVKUNOVA, O. B., 2001: Optical properties and nondestructive estimation of anthocyanin content in plant leaves. Photochem. Photobiol., 74(1): 38-45. DOI: 10.1562/0031-8655(2001)0742.0.CO;2 Go to original source...
    17. GITELSON, A. A., ZUR, Y., CHIVKUNOVA, O. B., MERZLYAK M. N., 2002: Assesing Carotenoid content in plant leaves with reflectance spectroscopy. Photochem. Photobiol., 75(3): 272-281. DOI: 10.1562/0031-8655(2002)0752.0.CO;2 Go to original source...
    18. JERMINI, M., BLAISE, P., GESSLER, C., 2010: Influence of Plasmopara viticola on gas exchange parameters on field-grown Vitis vinifera Merlot. Vitis, 49(2): 87-93.
    19. JIANG, Y., CARROW, R. N., 2005: Assesment of narrow-band canopy spectral reflectance and turfgrass performance under drought stress. Hort Science, 40(1): 242-245. Go to original source...
    20. KITAJIMA, M., BUTLER, W. L., 1975: Quenching of Chlorophyll fluorescence and photochemistry in chloroplasts by dibromothymoquinone. Biochem. et Biophys. Acta (BBA)-Bioenergetics, 376(1): 105-115. DOI: 10.1016/0005-2728(75)90209-1 Go to original source...
    21. KORTEKAMP, A., ZYPRIAN, E., 2003: Characterization of Plasmopara- resistance in grapevine using in vitro plants. J. Plant. Physiol., 160: 1393-1400. DOI: 10.1078/0176-1617-01021 Go to original source...
    22. LAFON, R., CLERJEAU, M. (ED.), 1988: Downy mildew. Compedium of grape diseases. APS Press, Minesota, pp. 11-13.
    23. LEBEDA, A., SEDLÁŘOVÁ, M., PETŘIVALSKÝ, M., PROKOPOVÁ, J., 2008: Diversity of defence mechanisms in plant-oomycete interactions: a case study of Lactuca spp. and Bremia lactucae. Eur. J. Plant Pathol., 122: 71-89. DOI: 10.1007/s10658-008-9292-3 Go to original source...
    24. LEBEDA, A., SPENCER-PHILLIPS, P. T. N., COOKE, B. M., 2008: The downy mildew-Genetics, molecular biology and control. Eur. J. Plant Pathol., 122(1): 3-18. Go to original source...
    25. LE MAIRE, G., FRANCOIS, C., DUFRENE, E., 2004: Towards universal broad leaf chlorophyll indices using prospect simulated database and hyperspectral reflectance measurements. Remote Sens Environ, 89: 1-28. DOI: 10.1016/j.rse.2003.09.004 Go to original source...
    26. LICHTENTHALER, H. K., 1987: Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods enzymol., 148: 350-382. DOI: 10.1016/0076-6879(87)48036-1 Go to original source...
    27. LICHTENTHALER, H. K., BABANI, F., LANGSDORF, G., 2007: Chlorophyll fluorescence imaging of photosynthetic activity in the sun and shade leaves of trees. Photosynth. Res., 93: 235-244. DOI: 10.1007/s11120-007-9174-0 Go to original source...
    28. LODISH, H., BERK, A., LAWRENCE ZIPURSKY, S., MATSUDAIRA, P., BALTIMORE, D., DARNELL, J., 2000: Molecular cell biology, 4th edition. W.H. Freeman, New York.
    29. MACCIONI, A., AGATI, G., MAZZINGHI, P., 2011: New vegetation indices for remote measurement of chlorophylls based on leaf directional reflectance spectra. J. Photochem. Photobiol., 61(1-2): 52-61. DOI: 10.1016/S1011-1344(01)00145-2 Go to original source...
    30. MAHATHAMA, M. K., BHATNAGAR, R., DHANDHUKIA, P., THAKKAR, V. R., 2009: Variation in metabolites constituent in leaves of downy mildew resistant and susceptible genotypes of pearl millet. Physiol. Mol. Biol. Plants, 15(3): 249-255. DOI: 10.1007/s12298-009-0028-4 Go to original source...
    31. MANDAL, K., SARAVANAN, R., MAITI, S., KOTHARI, I. L., 2009: Effect of downy mildew disease on photosynthesis and chlorophyll fluorescence in Plantago Ovata Forsk. J. of Plant diseases and Protection, 116(4): 164-168. Go to original source...
    32. MAXWELL, K., JOHNSON, G. N., 2000: Chlorophyll fluorescence-a practical quide. J. Exp. Bot., 345(51): 659-668. DOI: 10.1093/jexbot/51.345.659 Go to original source...
    33. MERZLYAK, M. N., CHIVKUNOVA, O. B., 2000: Light-stress-induced pigment changes and evidence for anthocyanin photoprotection in apples. J. Photochem. Photobiol. B. Biol., 55: 155-163. DOI: 10.1016/S1011-1344(00)00042-7 Go to original source...
    34. MERZLYAK, M. N., GITELSON, A. A., CHIVKUNOVA, O. B., SOLOVCHENKO, A. E., POGOSYAN S. I., 2003: Application of reflectance spectroscopy for analysis of higher plant pigments. Russ. J. Plant Physiol., 50(5): 704-710. DOI: 10.1023/A:1025608728405 Go to original source...
    35. MERZLYAK, M. N., GITELSON, A. A., POGOSYAN, S. I., CHIVKUNOVA, O. B., LEHIMENA, L., GARSON, M., BUZULUKOVA, N. P., SHEVYREVA, V. V., RUMYANSTEVA, V. B., 1997: Reflectance spectra of leaves and fruits during their development and senescence under stress. Russ. J. Plant Physiol., 4(5): 614-622.
    36. MORINODO, M., ORLANDINI, S., GIUNTOLI, A., BINDI, M., 2005: The effect of Downy and Powdery Mildew on grapevine (Vitis vinifera L.) leaf gas exchange. J. Phytopathology, 153: 350-357. DOI: 10.1111/j.1439-0434.2005.00984.x Go to original source...
    37. MYNENI, R. B., HALL, F. G., SELLERS, P. J., MARSHAK, A. L., 1995: The interpretation of spectral vegetation indexes. IEEE Trans Geosci Remote Sensing, 33: 481-486. DOI: 10.1109/36.377948 Go to original source...
    38. NANDA, R. M., BISWAL, B., 2008: Biotic stress induced demolition of thylakoid structure and loss in photoelectron transport of chloroplasts in papaya leaves. Plant Physiol. Biochem., 46: 461-468. DOI: 10.1016/j.plaphy.2007.12.003 Go to original source...
    39. NEDBAL, L., WHITMARSH, J., 2004: Chlorophyll fluorescence imaging of leaves and fruits. In: Papageorgiu, G. C., Govinjee. (ed.): Chlorophyll a fluorescence" A signature photosynthesis. Pp. 389-407. Springer, Dodrecht - Netherlands. Go to original source...
    40. OXBOROUGH, K., 2004: Imaging of chlorophyll a fluorescence: theoretical and practical aspects of an emerging technique for the monitoring of photosynthetic performance. J. Exp. Bot., 400(55): 1195-1205. DOI: 10.1093/jxb/erh145 Go to original source...
    41. PAPAGEORGIOU, G. C., GOVINJEE (ED.), 2004: Chlorophyll a Fluorescence: A signature of Photosynthesis.- Springer, Dodrecht - Netherlands. Go to original source...
    42. PAPAGEORGIU, G. C., GOVINJEE, 2011: Photosystem II Fluorescence: Slow changes -scaling from the past. J. Photochem. Photobiol. B: Biol., 1-13. Go to original source...
    43. PROKOPOVÁ, J., ©PUNDOVÁ, M., SEDLÁŘOVÁ, M., HUSIČKOVÁ, A., NOVOTNÝ, R., DOLE®AL, K., NAU©, J., LEBEDA, A., 2010: Photosynthetic response of lettuce to downy mildew infection and cytokinin treatment. Plant Physiol. Biochem., 48: 716-723. DOI: 10.1016/j.plaphy.2010.04.003 Go to original source...
    44. ROHÁČEK, K., 2002: Chlorophyll fluorescence parameters: the definitions, photosynthetic meaning, and mutual relationship. Photosynthetica, 40(1): 13-29. DOI: 10.1023/A:1020125719386 Go to original source...
    45. ROHÁČEK, K., SOUKUPOVÁ, J., BARTÁK, M., 2008: Chlorophyll fluorescence: A wonderfull tool to study plant physiology and plant stress. Plant Cell Compartment- Selected topics, 41-104.
    46. ROLFE, S. A., SCHOLES, J.D., 2010: Chlorophyll fluorescence imaging of plant-pathogen interactions. Protoplasma, 247: 163-175. DOI: 10.1007/s00709-010-0203-z Go to original source...
    47. ROMMER, CH., BURLING, K., HUNSCHE, M., RUMPF, T., NOGA, G., PLUMER, L., 2011: Robust fitting of fluorescence spectra for pre-symptomatic wheat leaf rust detection with Support Vector Machines. Comp. and El. in Agr., 79: 180-188. DOI: 10.1016/j.compag.2011.09.011 Go to original source...
    48. SOUKUPOVÁ, J., SMATANOVÁ, S., NEDBAL, L., JEGOROV, A., 2003: Plant response to destruxins visualized by imaging of chlorophyll fluorescence. Physiologia Plantarum, 118: 399-405. DOI: 10.1034/j.1399-3054.2003.00119.x Go to original source...
    49. USTIN, S. L, ROBERTS, D. A, GAMON, J. A, ASNER, G. P, GREEN, R. O., 2004: Using imaging spectroscopy to study ecosystem processes and properties. Bioscience 54: 523-534. DOI: 10.1641/0006-3568(2004)054[0523:UISTSE]2.0.CO;2 Go to original source...
    50. WU, CH., NIU, Z., TANG, Q., HUANG, W., 2008: Estimating chlorophyll content from hyperspectral vegetation indices: Modelling and validation. Agric. Forest Met., 148: 1230-1241. DOI: 10.1016/j.agrformet.2008.03.005 Go to original source...

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