Acta Univ. Agric. Silvic. Mendelianae Brun. 2012, 60(8), 191-198 | DOI: 10.11118/actaun201260080191

Evaluation of foliar resistance of grapevine genetic resources to downy mildew (Plasmopara viticola)

Pavel Pavloušek
Ústav vinohradnictví a vinařství, Mendelova univerzita v Brně, Valtická 337, 691 44, Lednice, Czech Republic

In this study altogether the resistance of 44 grapevine cultivars to downy mildew was evaluated in a long-term field experiment (1996-2003). The resistance was evaluated under conditions of a natural infection pressure using the OIV 452 scale as well as on the base of the evaluation of disease incidence of sporulation and disease incidence of necrosis. The highest degree of resistance to downy mildew was found out in resistance donors Seyve Villard 12 375, Seibel 13 666 and Bianca cultivar. High correlations existing among individual methods of evaluation indicated a good applicability of all tested methods. The evaluation of disease incidence of sporulation and disease incidence of necrosis demonstrated the dependence of traits associated with the resistance to downy mildew on the colour of berries. When evaluating the correlation existing between the resistance and pedigree, it was demonstrated that the highest average resistance existed in cultivars that had the cultivar Seyve Villard 12 375 as the donor of resistance in their pedigree. Basing on obtained results it can be concluded that the OIV 452 scale can be considered as the basic method of resistance evaluation to downy mildew, above all in cases of a long-term evaluation under field conditions.

Keywords: grapevine, genetic resource, resistance, downy mildew, OIV scale
Grants and funding:

My sincere thanks for support belong to the Ministry of Agriculture of the Czech Republic. This publication was written as a part of the National Programme on Conservation and Utilization of Plant Genetic Resources and Agro-biodiversity.

Received: September 13, 2012; Published: March 30, 2013  Show citation

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Pavloušek, P. (2012). Evaluation of foliar resistance of grapevine genetic resources to downy mildew (Plasmopara viticola). Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis60(8), 191-198. doi: 10.11118/actaun201260080191
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References

  1. ALLEWELDT, G., 1996: Genetics of grapevine breeding. Progress of Botany, 58, 441-454. Go to original source...
  2. ALONSO-VILLAVERDE, V., VIRET, O., GINDRO, K., 2011: Downy mildew: is resistance linked to inoculums concentration? Vitis, 50, 127-129.
  3. BARLASS, M., MILLER, R. M., ANTCLIFF, A. J., 1986: Development of methods for screening grapevines for resistance to infection by downy mildew. I. Dual culture in vitro. American Journal of Enology and Viticulture, 37, 61-66. Go to original source...
  4. BOSO, S., MARTÍNEZ, M. C., UNGER, S., KASSEMEYER, H. H., 2006: Evaluation of foliar resistance to downy mildew in different cv. Albarino clones. Vitis, 45, 23-27.
  5. BOSO, S., KASSEMEYER, H. H., 2008: Different susceptibility of European grapevine cultivars for downy mildew. Vitis, 47, 39-49.
  6. BOSO, S., ALONSO-VILLAVERDE, V., GAGO, P., SANTIAGO, J. L., MARTÍNEZ, M. C., 2011: Susceptibility of 44 grapevine (Vitis vinifera L.) varieties to downy mildew in the field. Australian Journal of Grape and Wine Research, 17, 394-400. DOI: 10.1111/j.1755-0238.2011.00157.x Go to original source...
  7. BROWN, M. V., MOORE, J. N., FENN, P., MC NEW, R. W., 1999a: Evaluation of grape germplasm for downy mildew resistance. Fruit Variety Journal, 53, 22-29.
  8. BROWN, M. V., MOORE, J. N., FENN, P., MC NEW, R. W., 1999b: Comparison of leaf disc, greenhouse and field screening procedures for evaluation of grape seedlings for downy mildew resistance. HortScience, 34, 331-333. Go to original source...
  9. BROWN, M. V., MOORE, J. N., MC NEW, R. W., FENN, P., 1999c: Inheritance of downy mildew resistance in table grapes. Journal of American Society of Horticultural Science, 124, 262-267. Go to original source...
  10. CADLE-DAVIDSON, L., 2008: Variation Within and Between Vitis spp. For Foliar resistance to the Downy Mildew Pathogen Plasmopara viticola. Plant Disease 92, 1577-1584. DOI: 10.1094/PDIS-92-11-1577 Go to original source...
  11. EIBACH, R., DIEHL, H., ALLEWELDT, G., 1989: Investigations on the heritability of resistance to Oidium tuckeri, Plasmopara viticola and Botrytis cinerea in grapes. Vitis, 28, 209-228.
  12. DAI, G. H., ANDARY, C., MONDOLOT-COSSON, L., BOUBALS, D., 1995: Histochemical studies on the interaction between three species of grapevine, Vitis vinifera, V. rupestris and V. rotundifolia abd downy mildew fungus, Plasmopara viticola. Physiol. Mol. Plant. Pathol., 46, 177-188. DOI: 10.1006/pmpp.1995.1014 Go to original source...
  13. EIBACH, R., 1994: Investigations about the genetic resources of grapes with regard to resistance characteristics to powdery mildew, Oidium tuckeri. Vitis, 33, 143-150.
  14. GESSLER, C., PERTOT, I., PERAZZOLLI, M., 2011: Plasmopara viticola: a review of knowledge on downy mildew of grapevine and effective disease management. Phytopathologia Meditterranea, 50, 3-44.
  15. GINDRO, K., SPRING, J. L., PEZET, R., RICHTER, H., VIRET, O., 2006: Histological and biochemical criteria for objective and early selection of grapevine cultivars resistant to Plasmopara viticola. Vitis, 45, 191-196.
  16. KIEFER, B., RIEMANN, M., BÜCHE, C., KASSEMEYER, H.-H., NICK, P., 2002: The host guides morphogenesis and stomatal targeting in the grapevine pathogen Plasmopara viticola. Planta, 215, 387-393. DOI: 10.1007/s00425-002-0760-2 Go to original source...
  17. KOZMA, P., 2000: Winegrape breeding for fungus disease resistance. Acta Horticulturae 528, 505-510. Go to original source...
  18. LIU, S., SYKES, S. R., CLINGELEFFER, P. R., 2003: A method using leafed single-node cuttings to evaluate downy mildew resistance in grapevine. Vitis, 42, 173-180.
  19. LIU, S. M., SYKES, S. T., CLINGELEFFER, P. R., 2008: Variation between and within grapevine families in reaction to leaf inoculation with downy mildew sporangia under controlled conditions. Vitis, 47, 55-63.
  20. MOREIRA, F. M., MADINI, A., MARINO, R., ZULINI, L., STEFANINI, M., VELASCO, R., KOZMA, P., GRANDO, M. S., 2011: Genetic linkage maps of two interspecific grape crosses (Vitis spp.) used to localize quantitative trait loci for downy mildew resistance. Tree Genetics and Genomes, 5, 153-167. DOI: 10.1007/s11295-010-0322-x Go to original source...
  21. OIV, 1983: Descriptor list for grapevine varieties and Vitis species. OIV Paris, 135.
  22. PATIL, S. G., HONRAO, B., RAO, V. G., PATILl, V. P., 1989: Evaluation of grape germplasm for downy mildew resistance and its significance in breeding. Indian Journal of Horticulture, 46, 476-479.
  23. PERESSOTTI, E., WIEDEMANN-MERDINOGLU, S., DELMOTTE, F., BELLIN, D., DI GASPERO, G., TESTOLIN, R., MERDINOGLU, D., MESTRE, P., 2010: Breakdown of resistance to grapevine downy mildew upon limited deployment of a resistant variety. BMC Plant Biol. 10: 147. DOI: 10.1186/1471-2229-10-147. DOI: 10.1186/1471-2229-10-147 Go to original source...
  24. STAUDT, G., KASSEMEYER, H. H., 1995: Evaluation of downy mildew (Plasmopara viticola) resistance in various accessions of wild Vitis species. Vitis, 34, 225-228.
  25. TÖPFER, R., HAUSMANN, L., HARST, M., MAUL, E., ZYPRIAN, E., EIBACH, R., 2011: New Horizons for Grapevine Breeding. Fruit, Vegetable and Cereal Science and Biotechnology, 5, Special Issue, 79-100.

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