Acta Univ. Agric. Silvic. Mendelianae Brun. 2012, 60(8), 141-144 | DOI: 10.11118/actaun201260080141

The effect of media composition on multiplication of grape rootstocks in vitro

Břetislav Křižan, Eva Ondrušiková, Jana Moudrá
Mendeleum - Ústav genetiky, Mendelova univerzita v Brně, Valtická 334, 691 44 Lednice, Czech Republic

The current demand for in vitro cultures of grape rootstocks, not only for mass production of plants, but also for genetic engineering is evident. The study on micropropagation of grape rootstock genotypes namely Kober 5BB, Kober 125AA and Teleki 5C was performed. The aim of the study was to develop an optimized protocol to obtain large quantity of plant material. Protocol is based on regeneration via organogenesis, considering that grape embryogenic calluses are laborious to establish and the genotype of the regenerated plants can be altered.
Using of Driver and Kuniyuki Walnut media for the establishing of proliferating cultures gave better results than Murashige Skoog media in case of all used rootstocks. Subsequent cultivation on modified Murashige Skoog media with 1-naphtalene acetic acid and increased concentration of cytokynin was characterized by multiplication of cultures and formation of clusters with high multiplication capability. The clusters obtained from rootstock genotypes were suitable for mass propagation as well as for genetic transformation due to their high ability of regeneration.

Keywords: Vitis, in vitro propagation, plant growth regulators, rootstocks, regeneration
Grants and funding:

The financial support of the project NAZV QH91214 of the Czech Ministry of Agriculture is gratefully acknowledged.

Received: September 13, 2012; Published: March 30, 2013  Show citation

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Křižan, B., Ondrušiková, E., & Moudrá, J. (2012). The effect of media composition on multiplication of grape rootstocks in vitro. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 60(8), 141-144. doi: 10.11118/actaun201260080141
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    References

    1. ALIZADEH, M., SINGH, S. K., PATEL, V. B., 2010: Comparative performance of in vitro multiplication in four grape (Vitis spp.) rootstock genotypes. International Journal of Plant Production 4 (1), pp. 41-50.
    2. BHOR, R. P., AHIRE, D. B., BAN, Y. G., BORSE, S. N. 2009: Study on micropropagation in grape (Vitis champini). Ecology, Environment and Conservation 15 (1), pp. 41-44.
    3. CELIK, H., SÖYLEMEZOGLU, G., ERTUNC, F., CAKIR, A., DURSUNOGLU, S., AKBAS, B., 2009: Clonal micropropagation of main grape and rootstock varieties of Turkish viticulture for obtaining virus-free basic nursery stocks. Acta Horticulturae 827, pp. 421-424. DOI: 10.17660/ActaHortic.2009.827.72 Go to original source...
    4. DRIVER, J. A., KUNIYUKI, A. H., 1984: In vitro propagation of paradox walnut rootstock. Hort. Sci. 19: 507-709. Go to original source...
    5. EL-AGAMY, S. Z., EL-MAHDY, T. K., MOHAMED, A. A., 2009: In vitro propagation of some grape rootstocks. Acta Horticulturae 839, pp. 125-131. DOI: 10.17660/ActaHortic.2009.839.14 Go to original source...
    6. GAMBORG, O. L., MILLER, A., OJIMA, K., 1968: Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151-158. DOI: 10.1016/0014-4827(68)90403-5 Go to original source...
    7. HELOIR, M. C., FOURNIOUX, J. C., OZIOL, L., BESSIS, R., 1997: An improved procedure for the propagation in vitro of grapevine (Vitis vinifera) using axillary bud microcuttings. Plant Cell Tissue Org. Cult. 49, 223-225. DOI: 10.1023/A:1005867908942 Go to original source...
    8. LLOYD G., MCCOWN B., 1981: Commercially-feasible micro-propagation of Mountain laurel, Kalmia latifolia, by use of shoot tip culture. Int. Plant Prop. Soc. Proc. 30: 421-427.
    9. LU, M. C., 2005: Micropropagation of Vitis thunbergii Sieb. et Zucc., a medicinal herb, through high-frequency shoot tip culture. Scientia Horticulturae 107: 64-69. DOI: 10.1016/j.scienta.2005.05.014 Go to original source...
    10. MARTINEZ, E. A., TIZIO, R., 1989: Grapevine micropropagation through shoot tips and minicuttings from in vitro cultured one-node cuttings. HortScience 24(3): 513. Go to original source...
    11. MURASHIGE T., SKOOG F., 1962: A revised medium for rapid growth and bio-assays with tobaco tissue cultures. Physiol. Plant. 15: 473-497. DOI: 10.1111/j.1399-3054.1962.tb08052.x Go to original source...
    12. NITSCH, J. P., NITSCH, C., 1969: Haploid plants from pollen grains. Science 163, 85-87. DOI: 10.1126/science.163.3862.85 Go to original source...
    13. SAJID, G. M., AHMED, Z., 2008: Evaluation of various levels of mineral nutrients and plant growth regulators for In vitro culture of grape. Pakistan Journal of Botany 40 (1), pp. 329-336.

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