Acta Univ. Agric. Silvic. Mendelianae Brun. 2012, 60(8), 67-72 | DOI: 10.11118/actaun201260080067

Effect of sample dilution on estimated values of antioxidant capacity by photochemiluminiscence method

Pavel Híc, Josef Balík
Ústav posklizňové technologie zahradnických produktů, Mendelova univerzita v Brně, Valtická 337, 691 44 Lednice, Czech Republic

This paper describes principles of a proper dilution of samples used for the determination of antioxidant capacity by means of a photochemiluminiscence method using the instrument Photochem (Analytik Jena AG). The authors used the method ACL (Lipid-soluble Antioxidant Capacity), which is one of two methods enabling to measure in this instrument. It was demonstrated that values measured by the photochemiluminiscence method ACL were influenced by the degree of sample dilution. When studying effects of dilution of samples of wine and rutin, it was demonstrated that there is a non-linear correlation between the degree of dilution and measured values of antioxidant capacity. At low molar amount of substance (i.e. at 0.5 and 1.0 nmol), the measured value of rutin inhibition was higher than the same that molar amount of trolox. At higher molar amounts (i.e. 3; 4 and 5 nmol), the inhibition value of trolox was higher. This dependent change can be explained by means of a different effectiveness of antioxidants and a different stability of their products with radicals.

Keywords: photochem, photochemiluminiscence, sample dilution, antioxidant capacity
Grants and funding:

This research was supported by Agency of the Ministry of Agriculture of the Czech Republic (Grant No. QI91B094).

Received: September 13, 2012; Prepublished online: March 29, 2013; Published: March 30, 2013  Show citation

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Híc, P., & Balík, J. (2012). Effect of sample dilution on estimated values of antioxidant capacity by photochemiluminiscence method. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 60(8), 67-72. doi: 10.11118/actaun201260080067
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