Acta Univ. Agric. Silvic. Mendelianae Brun. 2012, 60(5), 151-160 | DOI: 10.11118/actaun201260050151

Effects of creatine and vitamin E on muscle energetic metabolism, antioxidant stability and meat quality of pigs

Rudolf Lahučký1, Ondřej Bučko1, Peter Haščík2, Tibor Liptaj3
1 Katedra špeciálnej zootechniky, Slovenská poľnohospodárska univerzita v Nitre, Tr. A. Hlinku 2, 949 76 Nitra
2 Katedra hodnotenia a spracovania živočíšnych produktov, Slovenská poľnohospodárska univerzita v Nitre, Tr. A. Hlinku 2, 949 76 Nitra
3 Ústav analytickej chémie, Slovenská technická univerzita Bratislava, Radlinského 9, 812 37 Bratislava, Slovenská republika

The effects of supplementing the diet of pigs with creatine monohydrate (CMH) and vitamin E on blood plasma creatine concentration, vitamin E level in muscle, muscle energy metabolism, antioxidant capacity and meat (musculus longissimus dorsi) quality of pigs (DNA tested and negative on malignant hyperthermia) were investigated. Two treatments were used: supplementation with CMH alone (2 g.kg-1 of feed, 10 days before slaughter); and supplementation with both vitamin E (500 mg α-tocopherol.kg-1 of feed for minimum of 30 days) and CMH (2 g.kg-1 of feed, 10 days before slaughter). Pigs supplemented with CMH alone showed elevated plasma creatine concentration (P ≤ 0.05). Phosphorus nuclear magnetic resonance (31P NMR) measurements on post mortem (15 min.) muscle samples showed the highest phosphocreatine levels and ratio PCr/Pi (P ≤ 0.05) in CMH supplemented pigs alone and in combination with vitamin E. Dietary supplementation with vitamin E significantly (P ≤ 0.05) increased the concentration of α-tocopherol in meat. Supplementation with CMH alone or in combination with vitamin E resulted in higher (P = 0.07) a values of loin chops at 5 days of storage. Antioxidative capacity (measured as MDA production after incubation of longissimus muscle homogenates with Fe2+/ascorbate) was substantially improved by vitamin E and somewhat by CMH supplementation.

Keywords: meat quality, pork, creatine monohydrate, vitamin E
Grants and funding:

This research was partially supported by the Ministry of Agriculture in Bonn (Germany) and the Ministry of Agriculture in Bratislava (Slovak Republic) and DAAD (Deutscher Akademischer Austauschdienst) in Bonn.

Received: June 1, 2012; Prepublished online: July 24, 2013; Published: July 30, 2013  Show citation

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Lahučký, R., Bučko, O., Haščík, P., & Liptaj, T. (2012). Effects of creatine and vitamin E on muscle energetic metabolism, antioxidant stability and meat quality of pigs. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 60(5), 151-160. doi: 10.11118/actaun201260050151
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