Acta Univ. Agric. Silvic. Mendelianae Brun. 2012, 60(5), 103-108 | DOI: 10.11118/actaun201260050103

Staphylococcus aureus growth and enterotoxin production in different types of milk

Bohdana Janštová, Jr.1, L. Necidová, B. Janštová, L. Vorlová
1 Ústav hygieny a technologie mléka, Veterinární a farmaceutická univerzita Brno, Palackého tř. 1, 612 42 Brno, Česká republika

The aim of our study was to assess Staphylococcus aureus growth and the time of first detection of staphylococcal enterotoxins type A, B and C (SEA, SEB, SEC) in different type of milk, depending on the strain and storage conditions. Raw, pasteurized, and UHT milk were inoculated with three strains of S. aureus, and growth patterns were determined by the plate method in accordance with EN ISO 6888-1. Baird-Parker agar medium was used for the detection of S. aureus and the Enzyme Linked Fluorescent Assay (ELFA) used with a miniVIDAS analyzer tested the production of staphylococcal enterotoxins. The results of model experiments showed the dependence of the growth rate and subsequent production of staphylococcal enterotoxins on incubation (storage) temperature, S. aureus strain, and type of milk. A significant finding was that the growth of S. aureus and production of enterotoxins in raw milk was inhibited by natural microflora, and production of enterotoxins was therefore not detected in raw milk within 102 hours of storage either at 15 °C or 22 °C. The highest risk of SEs production is associated with secondary contamination of pasteurized and UHT milk when stored at room temperature, where production was first detected after 12 hours of incubation.

Keywords: Staphylococcus aureus, staphylococcal enterotoxins, milk, food safety
Grants and funding:

The study was supported by research grant MSM 6215712402 Veterinary aspects of food safety and quality and IGA 18/2012/FVHE.

Received: April 16, 2012; Prepublished online: July 24, 2013; Published: July 30, 2013  Show citation

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Janštová, B., Necidová, L., Janštová, B., & Vorlová, L. (2012). Staphylococcus aureus growth and enterotoxin production in different types of milk. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 60(5), 103-108. doi: 10.11118/actaun201260050103
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