Acta Univ. Agric. Silvic. Mendelianae Brun. 2008, 56(4), 177-182 | DOI: 10.11118/actaun200856040177
Efekt polymorfismu genu růstového hormonu (GHRH/HaeIII) na mléčnou užitkovost holštýnsko-fríských krav v Polsku
- 1 Laboratory of Ruminant Improvement, Department of Ruminant Science, Agricultural University of Szczecin, Judyma 10, 71-460 Szczecin, Poland
- 2 Department of Ruminant Science, Agricultural University of Szczecin, Judyma 10, 71-460 Szczecin, Poland
- 3 Plant Breeding "Strzelce", Branch of Borowo, Borowo 35, 64-020 Czempin, Poland
Cílem publikace bylo vyhodnocení vztahů mezi polymorfismem GHRH genu (GHRH/HaeIII) a znaky mléčné užitkovosti u holštýnsko-fríských krav v Polsku s využitím PCR-RFLP metod pro genotypování. Molekulární základ sledované mutace byl definován jako A→C transverze na intronu 2. Do sledování bylo zahrnuto celkem 220 dojnic. Získány byly následující frekvence: 0,0227 pro genotyp AA, 0,3227 pro AB, a 0,6546 pro BB; 0,1841 pro alelu GHRHA a 0,8159 pro alelu GHRHB. Vysoce signifikantní vztah byl potvrzen mezi GHRH/HaeIII a produkcí mléka. Pro krávy genotypu BB byla charakteristická vyšší produkce i obsah tuku (P ≤ 0,01).
dojnice, holštýn, mléčná užitkovost, somatoliberin
Effect of growth hormone-releasing hormone gene polymorphism (GHRH/HaeIII) on milk performance in polish holstein-friesian cows
The objective of this study was to evaluate the relationship between the polymorphism within GHRH gene (GHRH/HaeIII) and milk production traits for Polish Holstein-Friesian using PCR-RFLP technique for genotyping. The molecular background of this mutation was defined as the A→C transversion at the intron 2. A total of 220 cows were examined. The following frequencies were established: 0.0227 for genotype AA, 0.3227 for AB, and 0.6546 for BB; 0.1841 for the allele GHRHA and 0.8159 for GHRHB. A highly significant association was found between the GHRH/HaeIII polymorphism and milk performance. The BB-genotype cows were characterised by a higher fat yield and percentage (P ≤ 0.01).
Keywords: dairy cattle, milk production traits, PCR-RFLP, somatoliberin
Published: November 8, 2014 Show citation
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