Acta Univ. Agric. Silvic. Mendelianae Brun. 2008, 56(1), 173-178 | DOI: 10.11118/actaun200856010173

Vliv teploty na apoptózu eozinofilních granulocytů krve skotu in vitro

Petr Sláma1, Zbyšek Sládek1, Dušan Ryšánek2, Ivana Burešová3
1 Ústav morfologie, fyziologie a genetiky zvířat, Mendelova zemědělská a lesnická univerzita v Brně, Zemědělská 1, 613 00 Brno, Česká republika
2 Výzkumný ústav veterinárního lékařství v Brně, Hudcova 70, 621 00 Brno, Česká republika
3 Lékařská fakulta, Masarykova univerzita, Univerzitní kampus Bohunice, Kamenice 5, 625 00 Brno, Česká republika

Cílem studie bylo zhodnotit vliv teploty na apoptózu eozinofilních granulocytů krve skotu in vitro. Heparinizovaná bovinní krev byla inkubována 1, 4 a 24 hodin při následujících teplotách: 4, 23 a 37 °C. UV záření bylo použito jako pozitivní kontrola apoptózy. Apoptóza eozinofilních granulocytů byla detekována průtokovým cytometrem po simultánním barvení Annexinem-V a propidium jodidem. Z vybraných teplot indukují 4 °C apoptózu eozinofilních granulocytů nejméně. Procento apoptotických eozinofilních granulocytů bylo následující: 1,65 ± 0,46 %; 1,76 ± 0,36 %; 4,78 ± 1,70 % po 1, 4 a 24 hodinách inkubace.

eozinofil, apoptóza, teplota, krev

The effect of temperature on apoptosis of bovine blood eosinophil granulocytes in vitro

The aim of the study was to evaluate the effect of temperature on apoptosis of bovine blood eosinophil granulocytes in vitro. Heparinised bovine blood was incubated for 1, 4 and 24 h under following temperatures: 4, 23 and 37 °C. UV irradiation was used as positive control of apoptosis. Eosinophil granulocytes apoptosis was detected by flow cytometry after simultaneous staining with Annexin-V and propidium iodide. From selected temperatures, 4 °C induced the eosinophil granulocytes apoptosis least. The proportion of apoptotic eosinophil granulocytes amounted to (mean ± SD) 1.65 ± 0.46%; 1.76 ± 0.36%; 4.78 ± 1.70% after 1, 4 and 24 h incubation, respectively.

Keywords: eosinophil, apoptosis, temperature, blood
Grants and funding:

This study was supported by Ministry of Agriculture of the Czech Republic (MZE 0002716201).

Received: September 20, 2007; Published: November 17, 2014  Show citation

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Sláma, P., Sládek, Z., Ryšánek, D., & Burešová, I. (2008). The effect of temperature on apoptosis of bovine blood eosinophil granulocytes in vitro. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 56(1), 173-178. doi: 10.11118/actaun200856010173
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