Acta Univ. Agric. Silvic. Mendelianae Brun. 2007, 55(5), 223-228 | DOI: 10.11118/actaun200755050223

Vhodnost termínu odběru vzorku révy vine pro testování GFLV a ArMV využitím ELISA metody

Pavel Pavloušek
Ústav vinohradnictví a vinařství, Mendelova zemědělská a lesnická universita v Brně, Valtická 337, 691 44 Lednice na Moravě, Česká republika

Při hodnocení výskytu virových chorob metodou ELISA je u nepovirů velmi významný termín odběrů vzorků. Cíle této práce bylo vyhodnotit výskyt GFLV a ArMV v různých fenofázích a různých částech rostliny.
Z pohledu obou virových chorob se zdá jako nejvhodnější termín odběru stadium B (intenzivní růst - růstový vrchol).

réva vinná, virové choroby révy vine, GFLV, ArMV, metoda ELISA, vizuální příznaky, termín odběru

Suitability of the date of grapevine tissue sampling for tests of GFLV and ArMV presence using ELISA method

Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV) are two important agents from the Nepoviruses genus. At present, serodiagnostic method is one of routine methods of virus pathogens testing also in grapevines. The aim of this study was to determine a suitable date of grapevine tissue sampling using the ELISA method and to follow visible symptoms of GFVL and ArMV infestation of vines. Positive results of tests for the occurrence of GFLV were obtained in the following growth stages: B (intensive growth - growth apex), C (beginning of flowering - young leaves) and D (beginning of flowering - inflorescence). Samples evaluated by ELISA method as positive for ArMV were found out in growth stages B (intensive growth - growth apex) and C (beginning of flowering - inflorescence). In all other stages the results of performed tests were negative. It is concluded that the stage B (intensive growth - growth apex) seems to be the most suitable for sampling of grapevine tissue.

Keywords: grapevine, grapevine virus disease, GFLV, ArMV, ELISA method, visual symptoms, term of sampling
Grants and funding:

This research was performed within the framework of QC0048 grant and was supported by the Czech Ministry of Agriculture. The authors wish to express their thanks for this support.

Received: December 10, 2006; Published: November 26, 2014  Show citation

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Pavloušek, P. (2007). Suitability of the date of grapevine tissue sampling for tests of GFLV and ArMV presence using ELISA method. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 55(5), 223-228. doi: 10.11118/actaun200755050223
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