Acta Univ. Agric. Silvic. Mendelianae Brun. 2006, 54(2), 131-138 | DOI: 10.11118/actaun200654020131

Analýza ovčího mléka FT NIR spektrometrem; měření vzorků na Petriho misce reflektanční kyvetou

Květoslava Šustová1, Jan Kuchtík2, Stanislav Kráčmar3
1 Ústav technologie potravin, Mendelova zemědělská a lesnická univerzita v Brně, Zemědělská 1, 613 00 Brno, Česká republika
2 Ústav chovu a šlechtění zvířat, Mendelova zemědělská a lesnická univerzita v Brně, Zemědělská 1, 613 00 Brno, Česká republika
3 Ústav výživy zvířat a pícninářství, Mendelova zemědělská a lesnická univerzita v Brně, Zemědělská 1, 613 00 Brno, Česká republika

Práce se zabývá možností stanovení základních složek ovčího mléka (sušiny, tuku, čistých bílkovin, kaseinu, laktózy a močoviny) FT NIR spektrometrem. Vzorky mléka byly před vlastním měřením zahřáty na teplotu 40 °C, protřepány a ochlazeny na 20 °C. Poté byly umístěny na Petriho misku a proměřeny kovovým zrcátkem na integrační sféře. Výsledky kalibračních korelačních koeficientů: sušina 0,983; tuk 0,989; čisté bílkoviny 0,997; kasein 0,977; laktóza 0,980 a močovina 0,973. Hotové kalibrační modely byly ověřeny křížovou validací. Z-testem nebyly zjištěny rozdíly mezi referenčními hodnotami a hodnotami naměřenými NIR spektrometrem (p=0,05). Na základě uvedených výsledků vyplývá, že NIR spektrometr je možno použít k rychlé analýze základních složek ovčího mléka.

blízká infračervená spektroskopie, ovčí mléko, sušina, tuk, bílkoviny, kasein, laktóza, chemické složení

Analysis of ewe's milk by FT Near Infrared spectroscopy: measurement of samples on Petri dishes in reflectance mode

Our work deals with a possibility of determination of basic composition (dry matter, fat, protein, casein, lactose and urea nitrogen) of ewe's milk and colostrum by FT NIR spectroscopy. Samples of milk were warmed to 40 °C, agitated, cooled to 20 °C, transferred into Petri dishes and analysed by reference methods and by FT NIR in reflectance mode. The measured area was spaced by a metallic mirror. Statistically significant differences between the reference values and the calculated values of NIR were not found (p=0.05). Results of calibration for ewe's milk determined the highest correlation coefficients: dry matter 0.983, fat 0.989, true protein 0.997, casein 0.977, lactose 0.980 and urea nitrogen 0.973. The study showed that NIRS method, when samples of milk are measured on Petri dishes, is a useful technique for the prediction of dry matter, fat, protein and casein in ewe's milk.

Keywords: near-infrared spectroscopy, ewe's milk, dry matter, fat, protein, lactose, chemical composition

Received: October 18, 2005; Published: December 12, 2014  Show citation

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Šustová, K., Kuchtík, J., & Kráčmar, S. (2006). Analysis of ewe's milk by FT Near Infrared spectroscopy: measurement of samples on Petri dishes in reflectance mode. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 54(2), 131-138. doi: 10.11118/actaun200654020131
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